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A kind of screening method of agarwood and fragrant bacteria

A screening method and technology for bacterial strains, which are applied in the field of screening of agarwood-bearing incense strains, can solve problems such as effective screening of non-agarwood strains, and achieve the effects of good incense quality, strong infectivity, and high leaching content.

Active Publication Date: 2016-04-20
广州道和堂中医药发展有限公司
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Problems solved by technology

Scientific research has initially found that some fungi can induce Agarwood to produce agarwood, but so far, there is no effective screening method for Agarwood strains, and no efficient induction of active fungi has been used in actual production.

Method used

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Embodiment Construction

[0014] The present invention will be further described in detail below in conjunction with the examples, but the embodiments of the present invention are not limited thereto.

[0015] A screening method for the Agarwood Agarwood strains, which is divided into: fungal inoculation, sampling, three indicators of the content of extracts in the Agarwood Agarwood region, the stained area of ​​the hole, and the colonization rate of the strains to promote the Agarwood Agarwood Agarwood. Dominant bacteria.

[0016] The concrete operation step of the inoculation of described fungus is, selects the white wood fragrant tree of trunk diameter more than 15cm of tree age more than 8 years, uses measuring tape to measure the distance and marks before punching, so that accurate drilling position, uses drill bit to 0.6mm percussion drill, drill 0.6cm~1.5cm in diameter and 2~6cm deep inoculation holes on the trunk, and drill 8 holes in a row at a vertical interval of 6~10cm (numbered from bottom...

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Abstract

The invention aims at providing a method for screening an agilawood edgeworthia chrysantha strain, and belongs to the technical field of strain screening. The method is characterized by orderly comprising the following steps: inoculating and sampling fungi, screening dominant bacteria of facilitating aquilaria sinensis edgeworthia chrysantha by three indexes such as the extract content of an edgeworthia chrysantha region, the hole dyeing area and the bacteria colonization rate according to the flow. The agilawood edgeworthia chrysantha fungi are screened by adopting the method, and the strain which is strong in infection ability, high in extract content of the edgeworthia chrysantha region and good in edgeworthia chrysantha quality can be selected. Thus, an effective bacterium screening method is provided for research of facilitation of aroma production by fungi-mediated microbiotic edgeworthia chrysantha, and is popularized and used in production.

Description

technical field [0001] The invention relates to the technical field of strain screening, in particular to a screening method for strains of agarwood and agarwood. Background technique [0002] Agarwood is the resinous wood of Aquilaria sinensis (Lour.) Gilg, a plant of the family Daphneaceae. It is a traditional and precious traditional Chinese medicine in my country, India and other Southeast Asian countries. It is also often used to make high-end perfumes, high-end essences, etc. Regarding the production and acquisition of agarwood, there are many methods, but the problems encountered are basically the same, that is, the period of agarwood fragrance is very long, the quantity is small, and the quality is different, which is far from meeting the market demand. People have never stopped forming agarwood. Exploration of the mechanism. Many scholars at home and abroad have explored that the formation of natural agarwood is closely related to the induction of fungi. It is gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12Q1/04C12R1/645
Inventor 李海燕杨洋陈丽张玉宜刘瑞驹
Owner 广州道和堂中医药发展有限公司
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