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A method for the stable germination of conidia of tomato gray leaf spot and its use for artificial inoculation identification at the seedling stage

A technology of gray leaf spot fungus and conidia, applied in the field of tomato gray leaf spot fungus control, can solve the problems of low spore germination efficiency and unsuitable production, and achieve avoiding repeated propagation, stable germination rate, and strong operability Effect

Active Publication Date: 2022-01-28
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for the stable germination of conidia of tomato gray leaf spot S. lycopersici for the problem of low germination efficiency of spores of tomato gray leaf spot S. lycopersici and identification of artificial inoculation at seedling stage

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  • A method for the stable germination of conidia of tomato gray leaf spot and its use for artificial inoculation identification at the seedling stage
  • A method for the stable germination of conidia of tomato gray leaf spot and its use for artificial inoculation identification at the seedling stage
  • A method for the stable germination of conidia of tomato gray leaf spot and its use for artificial inoculation identification at the seedling stage

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specific Embodiment approach 1

[0029] A method for the stable germination of conidia of tomato gray leaf spot and for the identification of artificial inoculation at the seedling stage, comprising the following steps:

[0030] Step a, prepare gray leaf spot bacteria spore suspension;

[0031] Step b, artificial inoculation: Spray the spore suspension prepared in step 1 directly on the front and back of the leaves at the tomato seedling stage, grow in the dark, and after 3 to 5 days, screen tomato materials resistant to gray leaf spot.

[0032] A kind of screening method of gray leaf spot of tomato described in the present embodiment, step a prepares the method for the spore suspension of gray leaf spot bacteria comprising the following steps:

[0033] Step a1. Treatment of tomato leaves: select fresh tomato leaves without disease spots, rinse them with tap water first, then soak them in alcohol for 5-10 seconds, put them in mercuric chloride solution for 1 minute, and then rinse them with sterile water for ...

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Abstract

The invention relates to a method for the stable germination of conidia of tomato gray leaf spot and its use for artificial inoculation identification at the seedling stage, which belongs to the field of tomato gray leaf spot control. The invention aims to solve the problems that the spore germination efficiency of the tomato gray leaf spot fungus is low and the spores are not suitable for production. The present invention prepares gray leaf spot fungus spore suspension and then artificially inoculates it, sprays the prepared spore suspension directly on the front and back of the leaves at the tomato seedling stage, grows in the dark, and after 3 to 5 days, carries out the tomato gray leaf spot resistance Material screening. The present invention inoculates the purified tomato gray leaf spot bacteria strain S. lycopersici on the PDA medium and cultivates it for 8-12 days, then picks the pathogenic bacteria on the medium and puts it into sterile tomato leaves to continue culturing for 1-2 weeks. The method can significantly increase the germination rate of the conidia of the tomato ash leaf spot, and establishes a simple and stable technical system for increasing the germination rate of the tomato pathogen conidia.

Description

technical field [0001] The invention belongs to the field of tomato gray leaf spot prevention and control, and in particular relates to a method for the stable germination of tomato gray leaf spot conidia and its use for artificial inoculation identification at the seedling stage. Background technique [0002] Tomato Gray Leaf Spot (Tomato Gray Leaf Spot) is mainly caused by the infection of S. lycopersici and S. solani S. solani. The leaves with gray leaf spot disease were collected from Chifeng in Inner Mongolia, Shouguang in Shandong, and Harbin in Heilongjiang for separation and purification. It indicated that the main species of local pathogen in Harbin was Stemphylium lycopersici, not Stemphylium solani. The disease occurs worldwide, but is severe in warm and humid regions. In recent years, tomato gray leaf spot has become a new disease that endangers tomato production in many provinces of my country, and has caused serious harm to tomato production. At present, the...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00C12Q1/18G01N2333/37
Inventor 杨欢欢姜景彬许向阳李景富赵婷婷张贺
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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