Needle biopsy tissue chip

A technology for puncture biopsy tissue and tissue chip, which is used in material inspection products, biological tests, etc., and can solve the problems of patents and literature reports without tissue chip technology and application.

Active Publication Date: 2014-06-18
泰州医药城博奥邦科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are no patents and literature reports on the technology and application of tissue chips prepared by biopsy tissue

Method used

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  • Needle biopsy tissue chip
  • Needle biopsy tissue chip
  • Needle biopsy tissue chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0010] Embodiment: The remaining paraffin tissue specimens of 42 breast cancer patients diagnosed as breast cancer by biopsy histopathology were collected, and the paraffin tissue chip of breast cancer biopsy tissue was prepared.

[0011] 1.1 Pathological diagnosis: breast biopsy tissue strips were fixed pathologically, dehydrated and embedded, and paraffin sections were stained for pathological diagnosis.

[0012] 1.2 Specimen selection: 42 paraffin tissue sections of breast cancer diagnosed by biopsy were selected, and the breast cancer lesion area (>1mm) on the HE-stained section was marked as the target tissue strip for tissue chip preparation.

[0013] 1.3 Paraffin core preparation: Guided by the mark of the breast cancer lesion area on the HE-stained section, use a paraffin knife to cut off the punctured tissue strip in the corresponding paraffin tissue block, and the length of the cut tissue strip is 1mm-4mm. Put the paraffin tissue block on a metal heating plate prehea...

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Abstract

The invention provides a technology and method for preparing high throughput needle biopsy tissue chip. The method comprises the following steps: collecting 42 residual paraffin-embedded tissue samples of needle biopsy of patients who have been diagnosed as the breast cancer patients through pathological examination on mammary gland needle biopsy tissues; taking the corresponding normal pathological HE stained sections as the guide, cutting the paraffin-embedded tissue sections (1mm to 4mm) corresponding to the cancer parts of the HE-stained sections, preparing paraffin-embedded tissue cores, preparing breast cancer needle biopsy tissue arrays by utilizing a tissue chip instrument, cutting paraffin into slices, and preparing paraffin-embedded tissue chip of breast cancer needle biopsy tissue. The prepared needle biopsy tissue chip can be applied to molecular medical research of related diseases such as immunocytochemistry, in-situ hybridization, and the like.

Description

technical field [0001] The invention belongs to the field of biochips, and relates to a biochip for preparing a microarray puncture biopsy tissue chip and a preparation method. Background technique [0002] Biochip refers to a high-throughput microarray of high-density tissues, cells, proteins, nucleic acids, sugars, and other biological components coated on solid supports such as silicon wafers and nylon membranes. Utilizing high-throughput biochips and specific molecular hybridization and staining techniques to detect the tested samples, a large amount of molecular detection information can be obtained accurately and quickly. At present, it has been widely used in the fields of life science research, molecular diagnosis of clinical medicine, and screening and verification of biological antibody-targeted drugs. [0003] Histopathological examination of needle biopsy has become one of the routine examination methods for clinical pathological diagnosis. The diameter of need...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 郜恒骏张可浩张新盛海辉颜虹张宇
Owner 泰州医药城博奥邦科生物科技有限公司
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