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Lactic acid bacteria elemental selenium product and production method thereof

A technology of elemental selenium and products, which is applied in the field of food biology and can solve problems such as the application of bacterial strains

Inactive Publication Date: 2014-07-02
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the safety of the strains, the above strains cannot be used in food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Lactobacillus brevis JLD715 transforms the characteristic of selenite

[0035] Combined with the manual figure 1 Example 1 will be described.

[0036] Lactobacillus brevis JLD715 was inoculated into an MRS medium with a sodium selenite concentration of 10 mM, cultured at 30 degrees Celsius for 5 days, the sludge was collected by centrifugation, and freeze-dried in a vacuum to obtain an orange-red powder. Samples were sent for scanning electron microscope analysis, and EDS energy spectrum analysis was performed on the surface of the bacteria, the results are shown in figure 1 , figure 2 . It was found that there were C, O and Se (0 valence) elements on the surface of the bacteria, combined with literature reports, it can be concluded that Lactobacillus brevis JLD715 produced elemental selenium. Through scanning electron microscopy combined with EDS energy spectrum analysis, elemental selenium was enriched on the surface of the bacteria.

Embodiment 2

[0037] Embodiment 2 Lactobacillus brevis is to the transformation rate of 0.2mM sodium selenite

[0038] MRS medium was used as the fermentation medium, and the starting strain was Lactobacillus brevis JLD715. After 0.2 mM sodium selenite was added to the sterilized MRS medium, 10% Lactobacillus brevis JLD715 was immediately inoculated and cultured aerobically at 30°C for 200 hours. The remaining selenite content was as follows: image 3 shown.

Embodiment 3

[0039] Embodiment 3 Lactobacillus brevis is to the transformation rate of 0.5mM sodium selenite

[0040] MRS medium was used as the fermentation medium, and the starting strain was Lactobacillus brevis JLD715. After 0.5 mM sodium selenite was added to the sterilized MRS medium, 10% Lactobacillus brevis JLD715 was immediately inoculated and cultured aerobically at 30°C for 250 hours. The remaining selenite content was as follows: image 3 shown.

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PUM

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Abstract

The invention discloses a lactic acid bacteria elemental selenium product and a production method thereof, and belongs to the field of food biotechnology. The invention discloses a method for obtaining the elemental selenium product by adding selenite into a lactobacillus brevis-containing system. The lactic acid bacteria elemental selenium product and the production method thereof have the following advantages: firstly, the elemental selenium product having food safety characteristics is provided, and secondly, the method applying lactic acid bacteria fermentation to produce the elemental selenium is provided.

Description

technical field [0001] The invention relates to a lactic acid bacteria elemental selenium product and a production method thereof, belonging to the field of food biotechnology. Background technique [0002] Selenium is a non-metallic chemical element that can be used as a photosensitive material, a catalyst in the electrolytic manganese industry, an essential nutrient element for animals and a beneficial nutrient element for plants. For humans or animals, although selenium enjoys the reputation of "longevity element", "king of anti-cancer", "natural antidote", etc., and its effect on health is irreplaceable by other substances, but high-concentration selenium has strong toxicity at the same time . The forms of selenium in nature are divided into: inorganic selenium (4-valent and 6-valent), organic selenium and elemental selenium (nano-selenium). There are significant differences in its utilization and cytotoxicity in different forms. Specifically, organic selenium has low...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/24
Inventor 姜毓君邓宇满朝新
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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