Safety evaluation method of transgenic insect-resistant rice against predatory natural enemy Auranthus praecox
A technique for evaluating the safety of Aurantia pratense and its safety, which is applied in the field of safety evaluation of transgenic insect-resistant rice against predatory natural enemy Aurantia sativa
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[0024] The Bt gene insect-resistant rice of the present invention selects the Bt gene insect-resistant rice gene rice T2A-1, which has high resistance to lepidopteran pests, and the non-transgenic parent selects Guangminghui 63 rice, which is Bt gene insect-resistant The rice gene rice T2A-1 seeds were all provided by the State Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University. Rice Minghui 63 and transgenic Bt gene insect-resistant rice T2A-1 were cultivated in Yoshida nutrient solution to a height of about 15 cm in a cultivation tray of length × width × height = 25 cm × 20 cm × 3 cm, and then used for evaluation tests. The conditions for cultivating rice seedlings are temperature (26±2° C.) and relative humidity (80%).
[0025] The tested brown planthoppers of the present invention were collected from paddy fields in Wuhan, Hubei, and established populations on rice seedlings of T2A-1 and Minghui 63 respectively indoors, and reared for more than 10...
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