Breeding method of single-grain beet high-resistance pairing sterile line

A paired and single-seed technology, applied in botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of poor disease resistance and low purity of sterile line seed fertility

CN103947546AInactive Publication Date: 2014-07-30石河子农业科学研究院
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2014-07-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a breeding method of a single-grain beet high-resistance pairing sterile line. The method comprises the steps of selecting high-resistance and single-grain beet base material, and carrying out summer seeding to cultivate a mother root; carrying out spatial isolation planting, inspecting the grain fertility of all single plants, separating single-grain sterile plants from single-grain fertile plants, and respectively listing to obtain seeds of the sterile plants and corresponding seeds of the fertile plants; carrying out centralized isolated hybridization on plant pairs, and harvesting to obtain first-filial generation F1 strain seeds; carrying out backcross selection by a backcross transformation method, and selecting the strain material with the single fertility rate higher than 95% in the sterile line; indentifying and selecting the cultivated single-grain paired male sterile material on a beet spring sowing disease garden land or a beet seed collecting disease garden land; carrying out disease resistance screening, selecting and remaining the plants with resistance, and harvesting the seeds in pairs. Compared with the prior art, the breeding method has the beneficial effects that the single-grain sterile line with high purity can be cultivated, the single grain rate reaches 99%, and the sterility rate reaches 95%. After being indentified, selected and cultivated on the disease garden land for a plurality of years, the single-grain beet pairing sterile line is high in resistance.
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Description

technical field

[0001] The invention relates to the technical field of plant breeding, in particular to a breeding method for a paired sterile line with high resistance to sweet beetroot. Background technique

[0002] Sugar beet high-resistance single-seed paired sterile lines are necessary resource materials for sugar beet breeding research at home and abroad. Foreign countries are rich in resources, but China is relatively in short supply, especially the sterile lines with stable seed fertility, outstanding yield and quality, and high resistance. Breeding resources are very scarce and seem particularly precious.

[0003] Existing overweight sugar beet breeding mainly uses backcrossing to screen the existing single-seed germplasm resources, and then selects paired single-seed sterile lines. The disadvantage of using this method of breeding is that the selected sterile line itself is not high in seed fertility purity and disease resistance, which cannot meet the needs of su...

Examples

Embodiment Construction

[0018] The present invention will be described in further detail below in conjunction with specific examples, but not as a limitation of the present invention.

[0019] In one embodiment of the present invention, the breeding method of the high-resistant paired sterile line of sugar beets comprises the following steps:

[0020] Step 1, in the first year, select the basic material of high-resistant single-grain beet, and summer sow to cultivate the mother root; the original code of the basic material: FC201, introduced in the USDA library of the United States Department of Agriculture through the Dutch expert Dr.Chris.Middleburg, material number: 20051021, which is referred to as FC201 in the embodiment.

[0021] Step 2, in the second year, plant in space isolation, investigate the seed fertility of each individual plant, use the separation selection method to separate the single sterile plant and the single fertile plant, and list separately to obtain the sterile plant seeds (...