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Method for extracting catalpol from traditional Chinese medicine rehmannia root

A technology of rehmannia and catalpol, applied in the field of biomedicine, can solve the problems of difficult enrichment, many organic solvents, and high cost, and achieve the effects of wide range of pharmacological effects, environmental friendliness, and low cost

Active Publication Date: 2014-07-30
DAKANG JUNBISHA XIANYANG SHAANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Relevant literature reports that catalpol, the hypoglycemic component in rehmannia glutinosa, is chemically unstable, and it is difficult to enrich a certain amount, and the separation and purification process of catalpol is complicated, and the use of many organic solvents is high, so it is not suitable for mass production of catalpol.

Method used

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  • Method for extracting catalpol from traditional Chinese medicine rehmannia root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for extracting catalpol from Chinese medicine rehmannia glutinosa comprises the following steps:

[0026] 1) Wash 10Kg of fresh rehmannia glutinosa, put it into a homogenizer, add 5L of deionized water, homogenize, add 10L of methanol to the obtained paste, stir for 1 hour and then filter.

[0027] 2) The filter residue was extracted once more with 5L of 50% methanol, and the two filtrates were combined and concentrated until the density reached 1.2 to obtain 1.5L of viscous liquid.

[0028] 3) Add 5L of 95% ethanol to the liquid, stir for 30 minutes, and pour out the resulting clear liquid when no more precipitates are formed. Concentrate until there is no alcohol, and the pigment contained in Rehmannia glutinosa will be separated out of the solution.

[0029] 4) After filtering the insoluble matter, the aqueous phase was extracted twice with 2L n-butanol, the n-butanol phase was combined, and the solvent was evaporated under reduced pressure to obtain 30 g...

Embodiment 2

[0032] The method for extracting catalpol from Chinese medicine rehmannia glutinosa comprises the following steps:

[0033] 1) Wash 20Kg of fresh rehmannia glutinosa, put it into a homogenizer, add 10L of deionized water, homogenate, add 20L of methanol to the obtained paste, stir for 2 hours and then filter.

[0034] 2) Extract the filter residue once more with 10L of 50% methanol, combine the two filtrates, concentrate until the density reaches 1.2, and obtain 3L of viscous liquid.

[0035] 3) Add 10L of 95% ethanol to the liquid, stir for 1 hour, and pour out the resulting clear liquid when no more precipitates are formed. Concentrate until there is no alcohol, and the pigment contained in Rehmannia glutinosa will be separated out of the solution.

[0036] 4) After filtering the insoluble matter, the water phase was extracted twice with 4L n-butanol, the n-butanol phase was combined, and the solvent was evaporated under reduced pressure to obtain 60 g of off-white crystals...

Embodiment 3

[0039] The method for extracting catalpol from Chinese medicine rehmannia glutinosa comprises the following steps:

[0040] 1) Wash 30Kg of fresh rehmannia glutinosa, put it into a homogenizer, add 15L of deionized water, homogenate, add 30L of methanol to the obtained paste, stir for 3 hours and then filter.

[0041] 2) The filter residue was extracted once more with 15L of 50% methanol, and the two filtrates were combined and concentrated until the density reached 1.2 to obtain 4.5L of viscous liquid.

[0042] 3) Add 10L of 95% ethanol to the liquid, stir for 90 minutes, and pour out the resulting clear liquid when no more precipitates are formed. Concentrate until there is no alcohol, and the pigment contained in Rehmannia glutinosa will be separated out of the solution.

[0043] 4) After the insoluble matter was filtered, the aqueous phase was extracted twice with 6L n-butanol, the n-butanol phase was combined, and the solvent was evaporated under reduced pressure to obta...

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Abstract

The invention discloses a method for extracting catalpol from traditional Chinese medicine rehmannia root, belonging to the technical field of biomedicines. The method comprises the following steps: 1) taking homogenate of fresh rehmannia root and water, further adding methanol, stirring and then, filtering to obtain filtrate; 2) further extracting filter residue with methanol solution once, merging filtrate obtained by filtering twice and concentrating to obtain viscous liquid; 3) adding ethanol solution into the liquid, stirring and pouring out obtained clear liquid when no precipitate is generated any more; concentrating the obtained clear liquid till no alcohol exists so as to separate out pigment contained in rehmannia root from solution; 4) filtering insoluble, extracting water phase with n-butanol twice, merging n-butanol phase and removing solvent by reduced pressure evaporation to obtain grey white crystals; 5) recrystallizing the crystals with water once to obtain white crystals, namely catalpol. The extraction method disclosed by the invention has the advantages of simple process, extensive sources of raw material traditional Chinese medicine rehmannia root, low cost and environment-friendly extraction preparation process and opens a broad market for developing novel catalpol health care products and medicaments.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for extracting catalpol from traditional Chinese medicine rehmannia glutinosa. Background technique [0002] Rehmannia glutinosa is the root tuber of Rehmannia glutinosa Libsch, a plant of the family Scrophulariaceae. It is one of the commonly used tonic traditional Chinese medicines, and it is widely used in clinical practice. According to literature reports, the chemical components of Rehmannia glutinosa are mainly glycosides, among which catalpol and other iridoid glycosides are the main ones. [0003] Catalpol is the main active ingredient in the traditional Chinese medicine Rehmannia glutinosa, which has the effect of lowering blood sugar. However, it is extremely unstable under natural conditions and will decrease by 60%-90% in the process of processing. Therefore, the efficient extraction of catalpol in Rehmannia glutinosa is also A rational util...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 史萌张天
Owner DAKANG JUNBISHA XIANYANG SHAANXI PROV
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