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Construction method for acute hyperuricemia tree shrew model

A hyperuricemia and construction method technology, which is applied in the field of animal model establishment, can solve problems such as acute hyperuricemia models that have not yet been seen, and achieve good application prospects, simple operation, and low dose effects

Active Publication Date: 2014-08-13
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Before this application was filed, there was no report on establishing an acute hyperuricemia model using tree shrews

Method used

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  • Construction method for acute hyperuricemia tree shrew model
  • Construction method for acute hyperuricemia tree shrew model
  • Construction method for acute hyperuricemia tree shrew model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: Determination of normal tree shrew serum uric acid value

[0042] Select 200 healthy tree shrews, Chinese and Burmese tree shrews of Dianxia species, SCXK (Dian) K2013-0001, SYXK (Dian) K2013-0001, body weight 122.69±14.4g, age 2-3 years old, including 114 females and 114 males. The average is 86. Fasting blood was collected, serum was separated within 2 hours (4000rpm, 4°C, 15min), and the uric acid (UA) detection kit of Nanjing Jiancheng Bioengineering Institute was used for detection. The detection method was carried out according to the kit instructions.

[0043] The results showed that the average normal value of blood uric acid in 200 adult tree shrews before administration was 175.76±53.14umol / L, wherein the average value of female animals was 168.98±53.31umol / L, and the average value of male animals was 185.81±50.44umol / L.

Embodiment 2

[0044] Example 2: Effectiveness experiment of OA-induced acute hyperuricemia in tree shrews

[0045] Select 18 healthy tree shrews, including 10 ♀, 8 ♂, body weight 120.6±11.2g, and randomly divide them into 3 groups with 6 animals in each group (n=6). The 3 groups are 1000mg / kg dose group, 100mg / kg dose group, 1% CMC-Na control group, intraperitoneal administration, venous blood collection at 0h, 2h, 4h, 12h, 24h, 36h after administration, blood samples were centrifuged at 4000rpm, 4°C, 15min within 2h after blood collection The serum was obtained, and then the serum uric acid value, serum urea nitrogen and serum creatinine value were detected according to the method of the kit instructions.

[0046] The results showed that the intraperitoneal injection of OA1000mg / kg and 100mg / kg could significantly increase the blood uric acid level of the tree shrew within 2 hours, and the blood uric acid level of the 1000mg / kg dose group increased from 133.54±26.39μmol / L to 453.01±96.94μ...

Embodiment 3

[0057] Example 3: OA-induced tree shrew acute hyperuricemia dose-effect relationship experiment

[0058] Group and dose

0h

1h

2h

4h

8h

24h

100mg / kg dose group

187.07±37.61

531.24±96.14*

601.45±77.80*

230.82±53.36

271.54±53.75

119.53±82.33

80mg / kg dose group

171.48±58.49

557.63±78.99*

553.74±106.08*

234.15±90.23

162.47±31.28

86.09±53.36

40mg / kg dose group

188.27±35.80

602.00±152.16*

482.72±123.07*

194.20±36.52

134.93±34.06

168.26±31.25

20mg / kg dose group

159.36±3.28

259.61±41.51*

185.87±27.53

179.58±81.88

188.33±82.1

104.64±33.98

5mg / kg dose group

179.44±5.57

175.96±28.57

161.71±34.28

183.66±38.31

154.33±39.25

105.86±23.00

1% CMC-Na control group

191.87±40.28

154.69±23.83

140.93±14.51

153.14±14.88

152.83±27.36

80.22±26.75

[0059] Select 36 healthy tree shre...

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Abstract

The invention relates to a construction method for an acute hyperuricemia tree shrew model. The method comprises the following steps: (1) selecting adult healthy tree shrew with body weight of 110 to 150 g and an age of 1 to 3 years; (2) respectively measuring fasting serum uric acid values of males and females of tree shrew selected in the step (1); (3) carrying out conventional breeding on the tree shrew selected in the step (1) and pretreating the tree shrew by grasping and releasing the tree shrew with hand; and (4) injecting the tree shrew treated in the step (3) with an oteracil potassium suspension so as to obtain the acute hyperuricemia tree shrew model, wherein the amount of the used oteracil potassium suspension is 40 mg / kg to 100 mg / kg on the basis of the body weight of the tree shrew. The model has typical disease symptoms, a quantity index has statistical significance, and serum urea nitrogen and serum creatinine values and histopathological results prove that such a model making dosage range is safe and does not result in influence on the liver and the kidney.

Description

technical field [0001] The invention belongs to a method for establishing an animal model, in particular to a method for establishing a tree shrew model of acute hyperuricemia. Background technique [0002] In recent years, with the improvement of people's living standards, the incidence of hyperuricemia has increased year by year, and it is often accompanied by hypertension, hyperlipidemia and diabetes. According to domestic and foreign literature reports, hyperuricemia is significantly related to coronary heart disease, hypertension, diabetes, stroke, etc., and it is an independent risk factor and an important prognostic predictor. hotspot. Whether it is the screening of drugs or the study of pathogenic mechanisms, there is an urgent need for a recognized scientific and reasonable animal model. [0003] There are mainly three types of animals currently used for animal models of hyperuricemia, primates, rodents, and birds. The best animals are undoubtedly primates, becaus...

Claims

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Application Information

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IPC IPC(8): A61K31/53
Inventor 唐东红叶尤松罕园园李桂珍杨光蕊贾学云李润平念昕
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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