Construction method of hyperuricemia model

Abstract

The invention belongs to the technical field of experimental model construction, and relates to a construction method capable of being used for quickly constructing a long-term and stable hyperuricemia model. A technological process of the method comprises the four steps of selecting samples, adaptively feeding, constructing the model, and applying the model, and concretely comprises the steps of adaptively feeding selected rats in an SPF animal feeding room for 3 to 7 days, observing body surface characteristics and behavioral activities of the rats, and reserving qualified sample rats; feeding the qualified sample rats with a yeast feed with the mass percentage being 20 percent and fructose water with the mass percentage being 10 percent or correspondingly proportional fresh fruit juice, and enabling the rats to take food and drink freely, thus obtaining the sample rat hyperuricemia model; applying the constructed rat hyperuricemia model, thus obtaining a model with representative illness representations. By adopting the method combining feed and drinking water for modelling, the operation is simple and easy to grasp, animal injuries caused by long-time gavage or injection are avoided, and the method is safe and reliable, and has a favorable application prospect.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of experimental model construction, and relates to a method capable of quickly constructing a long-term stable hyperuricemia model, in particular to a method for constructing a hyperuricemia model using rats as samples. [0003] Background technique: [0004] In recent years, with the improvement of people's living standards and changes in dietary patterns, the incidence of hyperuricemia has increased year by year; a number of epidemiological surveys have shown that hyperuricemia is the cause of cardiovascular disease, kidney disease and diabetes, etc. The independent risk factor of metabolic syndrome has become a health problem that threatens the quality of human life all over the world. Hyperuricemia is usually used as a sign of gout clinically. Among the patients with hyperuricemia, 5% to 12% of them will eventually develop gout. Benzbromarone, etc., but these drugs all have certain toxic and side...

AI Technical Summary

Problems solved by technology

Hyperuricemia is usually used as a sign of gout clinically. Among the patients with hyperuricemia, 5% to 12% of them will eventually develop gout. Benzbromarone, etc., but these drugs all have certain toxic and side effects, such as causing lipid peroxidation damage in the body, and causing greater damage to the skin and mucous membranes, hematopoietic system, kidneys and liver; therefore, new drugs for the treatment of gout Research has received high attention, and establishing a stable and continuous hyperuricemia model is an important prerequisite for research on hyperuricemia and gout

[0005] At present, the mechanism of the commonly used hyperuricemia model construction method generally involves supplementing exogenous uric acid, supplementing uric acid precursors, inhibiting uricase activity, and inhibiting renal uric acid excretion, etc., such as using yeast and purines such as adenine and hypoxanthine Substances, or direct administration of exogenous uric acid to build models; however, because rats, mice and other mammals that are often used as experimental subjects have uricase that humans lack, in medium and long-term experiments, the activity of uricase is activated, making the blood The uric acid level cannot be maintained at a high level; in recent years, the most commonly used hyperuricemia model drug at home and abroad, oxonate potassium, can competitively combine with uricase, inhibit the activity of uricase, and reduce the blood uric acid level in the body. It increases in a short period of time and can be maintained for a long time; however, due to its competitive inhibition mechanism, long-term administration is required, which can cause strong inflammatory reactions and other adverse effects on the kidneys of experimental animals; currently the most commonly used drugs to inhibit uric acid excretion are mainly For the anti-tuberculosis drugs ethambutol and niacin, the disadvantage of this model construction method is that substances such as ethambutol and niacin can cause liver damage

[0006] Epidemiological surveys have shown that the intake of fructose is increasing year by year, and there is a certain correlation between the increase in fructose consumption and the increase in the average level of blood uric acid in the population. In addition, high fructose diets are associated with hypertension, diabetes, atherosclerosis There is a certain relationship between the occurrence of metabolic diseases such as cirrhosis and abdominal obesity; some studies have shown that 10% fructose water can increase the blood uric acid level of rats, but the high uric acid level formed is extremely unstable, so it is difficult to serve as an ideal Animal models of hyperuricemia are widely used; and the method of using a single drug to build a model often cannot achieve good results due to its single mechanism of action. The hyperuricemia model lasts longer, the blood uric acid rises more obviously, and it is closer to the mechanism and actual situation of human hyperuricemia; therefore, the method of high purine-containing yeast combined with a high fructose diet is used to induce hyperuricemia Blood disease, forming a stable animal model, can provide good technical support for the research on uric acid metabolism, intervention effect and its mechanism, and it is also the subject task of current academic circles

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