Rat hyperuricemia model feed as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of experimental animals, and relates to a preparation method of a rat hyperuricemia model feed. The method comprises the following steps: accurately weighing basic feed and 0.15%-0.2% by mass of adenine, performing mixing, adding corn oil with stirring, performing cooling in a cooling barrel, adding water with stirring and mixing, and performing granulating by virtue of a granulator so as to obtain a soft granulated feed; and drying off moisture at low temperature to obtain the rat hyperuricemia model feed. The invention also provides the rat hyperuricemia model feed and application thereof. A rat hyperuricemia model provided by the invention does not cause serious organic damage to the kidney, successfully copies an animal model similar to thepathogenesis of human hyperuricemia, and provides a basis for subsequent experimental research of observing and treating animals with drugs. Rats are only fed with a pure feed without adopting a gavage means, the method accords with animal ethics, the rat hyperuricemia model is obtained, and a theoretical basis is provided for research of related diseases of human beings.

Description

technical field [0001] The invention belongs to the technical field of experimental animals, and in particular relates to a rat hyperuricemia model feed and a preparation method and application thereof. Background technique [0002] At present, people have a more comprehensive understanding of the metabolism of urate in the body, and some progress has been made in the genetics of gout. The blood uric acid level of organisms is mainly regulated by the renal transport system. Gout is a disorder of purine metabolism that leads to increased blood uric acid and / or decreased excretion of uric acid by the kidneys, resulting in the deposition of urate in tissues. The natural course of the disease is divided into asymptomatic hyperuricemia, acute gouty arthritis, intermittent Four stages of gout, chronic tophaceous gout, and occasionally gouty nephropathy. About 10% of patients with hyperuricemia will naturally develop gout. [0003] Among the animal models currently used for rese...

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Problems solved by technology

[0004] Aiming at the deficiencies of the above-mentioned prior art, the present invention provides a method for preparing a rat hyperuricemia model feed, the purpose is to solve the problem that in the prior art, when adenine is administered intragastrically at a dose of 300ppm, the rat kidney damage is very serious , the entire kidney is white in color and weighs about twice the size of a normal kidney. The structure of the kidney tissue is completely destroyed, and there is no possibility of reversal at all. It is impossible to observe the technical problems of drug therapy.

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