Sterile feed for sterile rats and mice and preparation method thereof

Abstract

The invention belongs to the technical field of experimental animals, and particularly relates to a preparation method of a sterile feed for sterile rats and mice. The preparation method comprises thesteps of accurately weighing a basic feed, 3.5% by mass of a mineral premix and 0.1% by mass of a composite vitamin, mixing, adding 6.99% by mass of polyunsaturated fatty acid, stirring, adding water, stirring for mixing, granulating by a granulator, and drying to remove water at a low temperature, and carrying out irradiation sterilization and high-temperature high-pressure sterilization to obtain the sterile feed. According to the preparation method, in a preparation process of a sterile feed for sterile rats and mice, minerals and vitamins are additionally added to the basic feed to ensuresupplement of lost nutrients after radiation sterilization and high-pressure steam sterilization, thereby ensuring stable growth of sterile rats and mice. The invention also provides a sterile feed for rats and mice prepared by the above method, which can completely meet requirements of sterile feed for rats and mice in sterile experiments through dual sterilization of irradiation sterilization and high-temperature high-pressure sterilization.

Description

technical field [0001] The invention belongs to the technical field of experimental animals, and in particular relates to a sterile feed for sterile mice and a preparation method thereof. Background technique [0002] Germ-free animals are animals that do not harbor any foreign life forms including bacteria, viruses, fungi and parasites. There are a large number of microorganisms in the ordinary environment, and germ-free animals must be kept in a space that does not contain microorganisms at all. Such a space does not exist in nature and must be constructed artificially. This artificially created device that can guarantee sterility is named an isolator. With the development of sterile animal research, the feed and feeding devices of sterile animals have also been continuously improved. Sterile animals do not carry any organisms inside and outside the body, and are raised in a device isolated from the outside world, which eliminates the interference of organisms on the ex...

AI Technical Summary

Problems solved by technology

[0003] For above-mentioned deficiencies in the prior art, the present invention provides the preparation method of the aseptic feed of aseptic rats and mice, purpose is to solve the structure and nutrient composition of the feed tissue that the high pressure steam sterilization method in the prior art damages greatly, can not Meet the requirements of the laboratory for sterile and nutritious feed; there are technical problems such as incomplete sterilization and difficult dosage control in irradiation sterilization

Images