A kind of preparation method of plant source targeting plant source lactic acid bacteria

Abstract

The invention specifically relates to a method for preparing plant-source-targeted plant-source lactic acid bacteria. Temperature control and adaptive domestication are carried out on it, and domestication adopts the method of combining time and temperature control, which greatly improves the high activity, safety, and adaptability of plant-source lactic acid bacteria preparation, and is easy to operate, reduces technical costs, and improves plant-source production. Adaptive capacity of lactic acid bacteria. A method for preparing plant-derived lactic acid bacteria targeting plant-derived lactic acid bacteria is as follows: (1) The plant-derived bacterial strain CCTCC No.M204051 that has passed the screening is subjected to conventional slant culture activation and seed culture to obtain a seed culture solution, and the seed culture solution is not higher than Temperature control at 45 degrees, adding citric acid with a volume percentage of 2%-5% of the seed culture solution for adaptive training; (2) then prepare a fermentation medium, and inoculate the Bacillus strain and the above-mentioned seed culture solution after sterilization , and then cut with a high-speed shearing machine to obtain bacterial colonies; (3) then carry out solar energy and external heat source fermentation on the bacterial colonies to obtain bacterial sludge; (4) perform film coating treatment and drying on the bacterial sludge.

Description

1. Technical field: [0001] The invention specifically relates to a method for preparing plant-source-targeted plant-source lactic acid bacteria. 2. Background technology: [0002] In the background technology, according to the traditional preparation method of plant-derived lactic acid bacteria in my country, it is difficult to meet the large demand for plant-derived lactic acid bacteria in the market. Plant-derived lactic acid bacteria can improve the micro-ecological balance of the human intestinal tract, prevent infection of intestinal pathogenic bacteria, and improve human immunity. .It can also be used for animal and plant nutrition and environmental protection. However, the vast majority of microbial preparations on the market are mostly bacillus and yeast. It is difficult to find traces of targeted plant-derived lactic acid bacteria. There is a pity that "the protagonist is missing and the supporting role is on stage". The origin is earlier than that in China, but it ...

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Problems solved by technology

[0002] In the background technology, according to the traditional preparation method of plant-derived lactic acid bacteria in my country, it is difficult to meet the large demand for plant-derived lactic acid bacteria in the market. Plant-derived lactic acid bacteria can improve the micro-ecological balance of the human intestinal tract, prevent infection of intestinal pathogenic bacteria, and improve human immunity. .It can also be used for animal and plant nutrition and environmental protection

However, the vast majority of microbial preparations on the market are mostly bacillus and yeast. It is difficult to find traces of targeted plant-derived lactic acid bacteria. There is a pity that "the protagonist is missing and the supporting role is on stage". The origin is earlier than that in China, but it cannot adapt to the trend of green consumption

There are two reasons why plant-derived lactic acid bacteria are almost out of stock in the market: ① it is difficult to obtain plant-derived lactic acid bacteria on a large scale, production is difficult, and technical costs are high; ② the more important reason is that it is difficult to store and easy to inactivate

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