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Sheep Zinc Semen Dilute Preservation Solution and Its Preparation Method
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A technology for preserving liquid and semen, applied in the field of animal reproduction, can solve problems such as prolonging the preservation time of semen, and achieve the effects of slowing sperm death, improving conception rate and low cost
Inactive Publication Date: 2016-04-13
赤峰市农牧科学研究院
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However, when the number of high-quality breeding sheep is limited and the semen demand area is relatively large, high-quality fresh semen needs to be transported for a long distance, which requires prolonged semen storage time. Sometimes it needs to be transported for more than 12 hours, and even storage is required in special cases. three or four days
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Embodiment 1
[0025] Embodiment 1: trisodium citrate dihydrate (C 6 h 5 Na 3 o 7 2H 2 O) 2.2g, fructose (C 6 h 12 o 6 ) 1.8g, zinc L-lysine (Zn(Lys) 2 2H 2 O) 1.0g, oxytocin (C 43 h 66 N 12 o 12 S 2 ) 20IU, fresh egg yolk centrifugation supernatant 10ml, fresh milk centrifugation 100.0ml.
[0026] The preparation method of the above-mentioned semen dilution preservation solution is as follows:
[0027] Depend on figure 1 It can be seen that take fresh milk, filter it through 8 layers of sterilized gauze, then autoclave at 110°C for 10 minutes, let it stand at room temperature, remove the milk skin with sterilized tweezers, centrifuge at 3000g at 4°C for 10 minutes, discard the upper layer and float The substance and the lower layer were precipitated, and the middle layer was absorbed to obtain the fresh milk centrate A. Accurately weigh 2.2g of trisodium citrate dihydrate, 1.8g of fructose, and 0.8g of zinc L-lysine, add it to 100.0ml of fresh milk centrate A, heat in a wate...
Embodiment 2
[0028] Embodiment 2: trisodium citrate dihydrate (C 6 h 5 Na 3 o 7 2H 2 O) 2.0g, fructose (C 6 h 12 o 6 ) 1.5g, zinc L-lysine (Zn(Lys) 2 2H 2 O) 0.8g, oxytocin (C 43 h 66 N 12 o 12 S 2 ) 15IU, fresh egg yolk centrifugation supernatant 12ml, fresh milk centrifugation 100.0ml.
Embodiment 3
[0029] Implementation 3: Trisodium citrate dihydrate (C 6 h 5 Na 3 o 7 2H 2 O) 2.8g, fructose (C 6 h 12 o 6 ) 2.5g, zinc L-lysine (Zn(Lys) 2 2H 2 O) 0.5g, oxytocin (C 43 h 66 N 12 o 12 S 2 ) 25IU, fresh egg yolk centrifugation supernatant 15ml, fresh milk centrifugation 100.0ml.
[0030] The manufacturers of the medicines used in the above-mentioned three embodiments are as follows:
[0031] Table 1 Drug manufacturers
[0032]
[0033] Dilute the preservation solution with the sheep semen in Example 1, dilute the fresh semen of the healthy Dorper ram by 3 times, store it at 4°C for 72 hours, and test its semen quality by conventional methods. The result is that the sperm motility rate is 0.72, top The body integrity rate was 0.88, and the plasma membrane integrity rate was 0.69. See Table 2.
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Abstract
The invention discloses zinc-containing sheep seminal fluid diluting storage liquid and a preparation method of the zinc-containing sheep seminal fluid diluting storage liquid. The zinc-containing sheep seminal fluid diluting storage liquid is prepared from the following raw materials: 1.8-2.9 grams of di-hydration trisodium citrate, 1.5-2.5 grams of fructose, 0.5-1.0 gram of L-lysine zinc, 15-25IU of oxytocin, 5-15ml of fresh yolk centrifugal supernate and 100.0ml of fresh milk centrifugate. The milk centrifugate adopted by the zinc-containing sheep seminal fluid diluting storage liquid contains various nutritional substances and trace elements which are required by sperm motion; the various types of sugars and the added fructose can well supply energy to sperms together so as to complement the energy consumption and prolong the survival time of the sperms; by the adding of the L-lysine zinc, the content of the L-lysine zinc and the content of zinc in the seminal fluid are increased, and diluted biochemical seminal plasma components are improved; the normal metabolism of seminal plasma is facilitated, the death of the sperms is delayed, the moving capacity of the sperms is improved, and the sperm activity is improved; meanwhile, the proper amount of zinc can protect acrosin from being hydrolyzed.
Description
technical field [0001] The invention belongs to the technical field of animal reproduction, and in particular relates to a semen dilution preservation solution and a preparation method thereof for improving the semen quality and fertilization rate of fresh goat semen after long-distance transportation. Background technique [0002] There are two main types of semen used in artificial insemination, namely fresh semen and frozen semen. The former is often referred to as fresh semen, and the latter is referred to as frozen semen. In order to improve the utilization rate of breeding rams and facilitate the storage and transportation of semen, some breeding farms and livestock improvement workstations often produce frozen semen for sheep breeding work. However, the equipment for producing frozen semen is expensive, liquid nitrogen needs to be replenished in time during storage and transportation, the technical requirements for semen thawing are relatively high, and the fertilizat...
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