A kind of preparation method and application of antrodia mycelium culture medium

A technology of Antrodia camphorata mycelium and culture medium formulation is applied in the field of biotechnology to achieve the effects of increasing effective content and increasing biomass

Active Publication Date: 2016-06-29
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After cooling the culture medium, add Antrodia camphorata strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select Juncao Ganoderma lucidum lees that are free from diseases and insect pests and rich in Ganoderma lucidum mycelium. After breaking the bag, crushing, and drying, weigh 20g and dissolve it in 1000mL of water. Concentrate to 30 mL, freeze at -80°C and put it into a vacuum drying oven to obtain a tan powdery solid, which is the Juncao Ganoderma lucidum extract.

[0023] Preparation of Antrodia antrodia mycelium medium in this example: 2.5% glucose, 0.5% peptone, 0.3% maltose, 0.3% yeast extract, 0.1% KH 2 PO 4 , 0.1%MgSO 4 ·7H 2 O, VB 1 Dissolve 0.1% and 0.1% Juncao Ganoderma lucidum extract in 1000mL of water, adjust the pH value to 5.5, dispense each 100mL into 250mL volumetric flasks, sterilize at 121°C for 20min, and cool down for later use.

[0024] Culture method: culture at 28°C, 120rpm, and dark for 16 days.

[0025] After measurement, the growth of mycelium was 8.67g / L (DW), the content of polysaccharide was 0.97%, and the content of triterpene was 0.28...

Embodiment 2

[0028] Select Juncao Ganoderma lucidum lees that are free from diseases and insect pests and rich in Ganoderma lucidum mycelium. After breaking the bag, crushing, and drying, weigh 20g and dissolve it in 1000mL water. Concentrate to 40 mL, freeze at -80°C and put it into a vacuum drying oven to obtain a tan powdery solid, which is the Juncao Ganoderma lucidum extract.

[0029] Preparation of Antrodia antrodia mycelium medium in this example: 3.0% glucose, 1.0% peptone, 1.0% maltose, 1.0% yeast extract, 0.3% KH 2 PO 4 , 0.3%MgSO 4 ·7H 2 O, VB 1 0.1% and 0.5% Juncao Ganoderma lucidum extracts were dissolved in 1000mL water, the pH value was adjusted to 5, each 100mL was dispensed into 250mL volumetric flasks, and sterilized at 121°C for 20min. After the culture medium was cooled, the strains of Antrodia camphorata were added.

[0030] Culture method: culture at 28°C, 120rpm, and dark for 16 days.

[0031] After measurement, the mycelium growth amount was 9.2g / L (DW), the p...

Embodiment 3

[0034] Select Juncao Ganoderma lucidum lees that are free from diseases and insect pests and rich in Ganoderma lucidum mycelium. After breaking the bag, crushing and drying, weigh 20g and dissolve it in 1000mL water. Concentrate to 20 mL, freeze at -80°C and put it into a vacuum drying oven to obtain a tan powdery solid, which is the Juncao Ganoderma lucidum extract.

[0035] Preparation of Antrodia antrodia mycelium medium in this example: 1.0% glucose, 0.5% peptone, 0.5% maltose, 0.5% yeast extract, 0.2% KH 2 PO 4 , 0.2%MgSO 4 ·7H 2 O, VB 1 0.1% and 0.1% Juncao Ganoderma lucidum extracts were dissolved in 1000mL water, the pH value was adjusted to 5.5, each 100mL was dispensed into 250mL volumetric flasks, and sterilized at 121°C for 20min. After the culture medium was cooled, the strains of Antrodia camphorata were added.

[0036] Culture method: culture at 28°C, 120rpm, and dark for 16 days.

[0037] After measurement, the growth of mycelium is 9.0g / L (DW), the conte...

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Abstract

The invention discloses a preparation method and application of antrodia antrodia mycelium medium, which is a liquid culture obtained by mixing Juncao Ganoderma lucidum extract, glucose, maltose, yeast extract, KH2PO4, MgSO4·7H2O and VB1 according to a certain ratio base. The invention has the characteristics of rapid germination, large amount and rapid growth of the mycelium of Antrodia camphorata, and the polysaccharide content of the mycelia can reach 0.43-0.97%, and the content of triterpenes is 0.2%-0.41%, which are respectively 2 times higher than the traditional culture medium. The method can provide a large amount of mycelium of Antrodia camphorata in actual production, and the preparation method is simple, highly repeatable, and easy to operate.

Description

technical field [0001] The invention relates to a preparation method and application of antrodia mycelium culture medium, belonging to the field of biotechnology. Background technique [0002] Antrodia Cinnamomea ( Antrodiacinnamomea ) is a unique medicinal fungus in Taiwan, my country. It has many functions such as liver protection, detoxification, anti-inflammation, and anti-oxidation. Cinnamomumkanehirai ) is the only host of Antrodia cintrodia. Due to the very small amount of its host in nature and the slow growth rate of Antrodia cintrodia, Antrodia cintrodia is known as "the ruby ​​in the forest". Studies have shown that the mycelium of Antrodia Cinnamomea has a very similar health care function to fruiting bodies of Antrodia Cinnamomea. In order to meet the growing demand of people for Antrodia Cinnamomea, the present invention screens Juncao Ganoderma lucidum extract, glucose, maltose, etc. Rapidly stimulating the growth of mycelia of Antrodia cinnamomea, increasin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 李晶林占熺谢长海罗红建林冬梅童金华王培丹邵恩斯
Owner FUJIAN AGRI & FORESTRY UNIV
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