Preparation method of isomaltooligosaccharide

A technology of isomaltooligosaccharide and glucoside, which is applied in the field of preparation of isomaltooligosaccharide, can solve the problems of low content of oligosaccharide and poor functionality of isomaltooligosaccharide, shorten reaction time, increase added value and save cost Effect

Active Publication Date: 2014-11-05
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above technical solutions all have the problem of low content of oligosaccharides above panose, isomaltotriose and tetrasaccharide, which leads to poor functionality of isomaltooligosaccharides

Method used

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  • Preparation method of isomaltooligosaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A preparation method of isomaltooligosaccharide, comprising the steps of:

[0043](1) Add water to adjust the concentration of the starch slurry to 10 Baume, adjust the pH value to 5.5, add high-temperature-resistant α-amylase, and the amount of high-temperature-resistant α-amylase is 0.6L per ton of starch raw material to prepare starch slurry;

[0044] (2) The starch slurry prepared in step (1) was kept in a laminar flow tank at 80°C for 40 minutes, and was liquefied by the first injection at 100°C and the second injection at 121°C to obtain a DE value of 30. % of liquefied liquid;

[0045] (3) Add α-glucosidyl transferase to the liquefied liquid prepared in step (2), the amount of α-glucosidyl transferase is 1 L per ton of starch raw material, react at 55°C for 20h, and inactivate the enzyme at 85°C, Prepare transglycoside sugar solution;

[0046] After testing, the mass percentages of various sugars in the total sugar in the transglycoside sugar liquid are: glucos...

Embodiment 2

[0053] A preparation method of isomaltooligosaccharide, comprising the steps of:

[0054] (1) Add water to adjust the concentration of starch slurry to 15 Baume, adjust the pH value to 6.2, add high temperature resistant α-amylase, the amount of high temperature resistant α-amylase is 1.0L per ton of starch raw material, and prepare starch slurry;

[0055] (2) The starch slurry prepared in step (1) was kept in a laminar flow tank at 90°C for 25 minutes, and was liquefied by the first injection at 110°C and the second injection at 140°C to obtain a DE value of 40. % of liquefied liquid;

[0056] (3) Add α-glucosidyl transferase to the liquefied liquid prepared in step (2), the amount of α-glucosidyl transferase is 1.5L per ton of starch raw material, react at 65°C for 30h, and inactivate the enzyme activity at 90°C , to obtain transglycoside sugar solution;

[0057] After testing, the mass percentages of various sugars in the total sugar in the transglycoside sugar liquid are...

Embodiment 3

[0064] A preparation method of isomaltooligosaccharide, comprising the steps of:

[0065] (1) Add water to adjust the concentration of the starch slurry to 13 Baume, adjust the pH value to 5.8, add high-temperature-resistant α-amylase, and the amount of high-temperature-resistant α-amylase is 0.8L per ton of starch raw material to prepare starch slurry;

[0066] (2) The starch slurry prepared in step (1) was kept in a laminar flow tank at 85°C for 35 minutes, and was liquefied by the first injection at 105°C and the second injection at 130°C to obtain a DE value of 35. % of liquefied liquid;

[0067] (3) Add α-glucosidyl transferase to the liquefied liquid prepared in step (2), the amount of α-glucosidyl transferase is 1.5L per ton of starch raw material, react at 58°C for 25h, and inactivate the enzyme at 87°C , to obtain transglycoside sugar solution;

[0068] After testing, the mass percentages of various sugars in the total sugar in the transglycoside sugar liquid are: g...

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Abstract

The invention relates to a preparation method of isomaltooligosaccharide. The preparation method comprises the following steps: (1) adding water into a starch raw material so as to regulate slurry, regulating pH and then adding high-temperature-resistant alpha-amylase, to obtain starch slurry; (2) insulating the starch slurry, and conducting secondary spray liquefaction to obtain liquefied liquor; (3) adding alpha-transglucosidase, reacting and then deactivating enzymes to obtain trans-glycosylation liquor; and (4) conducting decoloring, filtering, hybridization, chromatographic separation, concentration and drying to obtain the isomaltooligosaccharide. According to the preparation method, polysaccharides with glucoamylase degradation polymerization degree of more than or equal to 3 can be reduced, the reacted liquor mainly comprises constituents: polysaccharides such as panose, isomaltotriose, isomaltotetrose and the like with polymerization degree of more than or equal to 3, furthermore, the saccharification process can be omitted, the reaction time can be shortened by 20-30h, the production efficiency can be greatly improved, and the cost can be comprehensively saved by 10%.

Description

technical field [0001] The invention relates to a preparation method of isomaltooligosaccharide, which belongs to the technical field of functional sugar production. Background technique [0002] Isomaltooligosaccharide, also known as branched oligosaccharides, refers to a type of oligosaccharides in which the number of monosaccharides ranging from 2 to 6 is formed by the combination of glucose groups with α-1,6 glycosidic bonds. Different, in order to distinguish it from maltose, it is called isomaltooligosaccharide. [0003] Isomaltooligosaccharide has good physical and chemical properties and physiological functions, such as low sweetness, which is 40% to 50% of sucrose, and the sweetness is soft and delicious, and the taste is refreshing; it is difficult to be digested by gastric enzymes, and the calorie is low, basically does not increase Blood sugar and blood lipids; promote the proliferation of bifidobacteria in the human intestinal tract, and can inhibit the formati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P19/14C12P19/12C12P19/04
Inventor 邵先豹窦光朋干昭波杨腾腾苏静
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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