A kind of breeding method of nicotine-resistant Lucilia sericata strain

The invention relates to a technology of green fly and a cultivation method, which is applied in the field of insect cultivation, can solve the problems of lack of nicotine-resistant green fly species and the like, and achieves the effects of stable cultivation effect, appropriate nutritional components and smooth growth of larvae

Inactive Publication Date: 2016-12-07
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for cultivating nicotine-resistant Lucilia sericata strains in order to overcome the lack of nicotine-resistant Lucilia sericata species in the prior art. The method of the present invention is not affected by various factors that are not suitable for tobacco leaves Influence, can obtain stable cultivation effect

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  • A kind of breeding method of nicotine-resistant Lucilia sericata strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Screening of nicotine-resistant Lucilia sericata varieties from wild Lucilia sericata varieties

[0041] S01. When the weather is fine, prepare the trapping culture material, the trapping culture material is composed of the following components in weight percentage: bran 90%, imported fish meal 10%, and the above-mentioned The formulation is adjusted to a humidity of 60%.

[0042] S02. Put the culture material prepared above into a square plastic pot per 1 kg (length*width*height=45cm*30cm*12cm). At 8:00 in the morning on the same day, the plastic pots containing the culture material were placed next to a domestic garbage dump, and at 17:00 in the afternoon, the plastic pots were taken back and brought back to the indoor cultivation.

[0043] S03. Under indoor conditions (temperature 25°C, humidity 30%), cultivate the trapped wild fly eggs until the larvae are mature, and use the behavior of the food-free period of Lucilia sericata to separate the fly maggots...

Embodiment 2

[0046] Example 2 Screening out nicotine-resistant Lucilia sericata varieties from wild Lucilia sericata varieties

[0047] S01. When the weather is fine, prepare the trapping culture material, which is composed of the following components in weight percentage: 80% bran, 20% imported fish meal, and the above-mentioned The formulation is adjusted to a humidity of 60%.

[0048] S02. Put the culture material prepared above into a square plastic pot per 1 kg (length*width*height=45cm*30cm*12cm). At 8:00 in the morning on the same day, the plastic pots containing the culture material were placed next to a domestic garbage dump, and at 17:00 in the afternoon, the plastic pots were taken back and brought back to the indoor cultivation.

[0049] S03. Under indoor conditions (temperature 25°C, humidity 30%), cultivate the trapped wild fly eggs until the larvae are mature, and use the behavior of the food-free period of Lucilia sericata to separate the fly maggots from the formula (i.e....

Embodiment 3

[0052] Example 3 Screening out nicotine-resistant Lucilia sericata varieties from wild Lucilia sericata varieties

[0053] S01. When the weather is fine, prepare the trapping culture material, which is composed of the following components in weight percentage: 70% of bran, 30% of imported fish meal, and the above-mentioned The formulation is adjusted to a humidity of 60%.

[0054] S02. Put the culture material prepared above into a square plastic pot per 1 kg (length*width*height=45cm*30cm*12cm). At 8:00 in the morning on the same day, the plastic pots containing the culture material were placed next to a domestic garbage dump, and at 17:00 in the afternoon, the plastic pots were taken back and brought back to the indoor cultivation.

[0055] S03. Under indoor conditions (temperature 25°C, humidity 30%), cultivate the trapped wild fly eggs until the larvae are mature, and use the behavior of the food-free period of Lucilia sericata to separate the fly maggots from the formula...

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Abstract

The invention discloses a cultivation method of a nicotine-resistant lucilia sericata strain. According to the cultivation method, eggs of nicotine-resistant lucilia sericata are inoculated in a first formula material containing nicotine and cultivated till larvae mature and pupate; after pupae emerge, a second formula material is used for feeding the pupae into imagoes; the stable nicotine-resistant lucilia sericata strain can be obtained by means of continuous acclimation and cultivation over 20-30 generations. The first formula material is composed of, by weight, 70-90% of bran and 10-30% of imported fish meal, wherein the humidity of the bran and the imported fish meal is adjusted to be 60% in a nicotine water solution with the mass percent concentration of 0.3%. By means of the cultivation method, the nicotine water solution with the proper concentration is directly adopted for scientific compatibility with the bran and the imported fish meal, the first formula material successfully covers the bad smell and bitterness of the nicotine, nutritional ingredients are appropriate, smooth growth of the larvae of the lucilia sericata is guaranteed, and it is ensured that a stable cultivation effect is achieved.

Description

technical field [0001] The invention relates to the technical field of insect breeding, in particular to a method for cultivating a nicotine-resistant Lucilia sericata strain. Background technique [0002] Unsuitable fresh tobacco leaves refer to fresh tobacco leaves (moisture content 80-90%) that are not suitable for flue-cured tobacco production, and the waste or low-grade tobacco leaves (moisture content about 15%) produced during the production process of flue-cured tobacco mentioned in the literature. different. Unsuitable tobacco leaves usually refer to the lower and upper leaves that are not suitable for the cigarette industry and need to be removed in the field after the tobacco plants are normally capped and 2 to 3 milk foot leaves are removed, mainly including the bottom of the tobacco plants due to insufficient light and stunted growth , 2 lower leaves with insufficient inclusions, expected poor quality after roasting, and no roasting value, and 2 top leaves with...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/37A23K10/20A23K10/22A23K20/163A23K20/132
CPCA01K67/033A23K10/22A23K10/30A23K20/00A23K50/90Y02P60/87
Inventor 陈泽鹏胡新军
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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