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Red light environment based incubation method of diapause brine shrimp eggs

A kind of artemia egg, diapause technology, applied in the direction of animal husbandry, etc., to achieve the effect of improving hatching rate

Inactive Publication Date: 2015-01-21
TIANJIN OCEAN PAL CAROL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hatching solution is prepared by mixing artificial sea crystals with tap water

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water, and its salinity is 16g dissolved salt / liter; pH value is 8.1; the concentration of dissolved oxygen is >6mg / L; the water temperature is 28°C;

[0021] Red light is irradiated on the liquid surface of the hatching solution, and the light intensity of the red light is 2800 lux.

[0022] The density of the diapause Artemia eggs in the hatching solution is 1.2 mg / L; and the Artemia eggs of Kazakhstan are used.

[0023] After 24 hours of incubation, the hatching rate of Artemia eggs reached 80%.

Embodiment 2

[0025] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water, and its salinity is 16g dissolved salt / liter; pH value is 8.1; the concentration of dissolved oxygen is >6mg / L; the water temperature is 28°C;

[0026] Red light is irradiated on the liquid surface of the hatching solution, and the light intensity of the red light is 2800 lux.

[0027] The density of the diapause Artemia eggs in the hatching solution is 1.2 mg / L; and the shelled Artemia eggs of Kazakhstan are used.

[0028] After 24 hours of incubation, the hatching rate of Artemia eggs reached 85%.

Embodiment 3

[0030] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water. The salinity is 16g dissolved salt / liter; the pH value is 8.1; the concentration of dissolved oxygen is >6mg / L; the water temperature is 28°C; Tea polyphenols.

[0031] Red light is irradiated on the liquid surface of the hatching solution, and the light intensity of the red light is 2800 lux.

[0032] The density of the diapause Artemia eggs in the hatching solution is 1.2 mg / L; and the shelled Artemia eggs of Kazakhstan are used.

[0033] After 24 hours of incubation, the hatching rate of Artemia eggs reached 90%.

[0034] The Kazakhstan artemia ovum of shelling treatment used in embodiment two and embodiment three all obtains through following processing steps:

[0035] 1) Configuration of the shelling liquid: the main components of the she...

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Abstract

The invention provides a red light environment based incubation method of diapause brine shrimp eggs. The diapause brine shrimp eggs are incubated in incubation liquid with density 1-1.5mg / L. Dissolving salt in the incubation liquid reaches 15-17g / L. pH (potential of hydrogen) of the incubation liquid is 8-9. Oxygen concentration of the incubation liquid is larger than 6mg / L. Temperature of the incubation liquid is 27.5-28.5 degrees. Illumination intensity of the red light is 2500-3000Lux. According to the arrangement, the incubation of the diapause brine shrimp eggs is achieved under the red light environment, and meanwhile, the factors including the illumination intensity, the temperature, the pH value and the dissolved oxygen are controlled, and the incubation rate of the diapause brine shrimp eggs under the red light environment is increased to 80% or above within 24 hours.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a method for hatching diapause Artemia eggs under a red light environment. Background technique [0002] Artemia (Brine shrimp, Artemia), commonly known as Artemia, is a class of small crustaceans that live in high-salinity waters. The nauplii of Artemia that hatch for 1-2 days have a large amount of yolk, rich in protein, Fat is a good opening bait for larvae such as fish, shrimp and crab, which can greatly improve the survival rate of artificial breeding of fish and shrimp without polluting water quality. [0003] At present, people have different opinions on the salinity, pH, light intensity, and incubation time required for Artemia hatching. Therefore, most of the research on Artemia hatching focuses on the above aspects. No one has proposed the impact of different energy lights on Artemia hatching. For example, the effect of red light on hatching diapause Artemia eggs, and how to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 孙朦朦
Owner TIANJIN OCEAN PAL CAROL BIOTECH
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