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A method for hatching diapause Artemia eggs under ultraviolet light

An artemia egg and ultraviolet light technology, which is applied in animal husbandry and other directions, can solve the problems of low hatching rate of Artemia eggs, and achieve the effect of solving the low hatching rate and improving the hatching rate.

Active Publication Date: 2016-08-24
TIANJIN OCEAN PAL CAROL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The problem to be solved by the present invention is a method for hatching diapause Artemia eggs under ultraviolet light. This method not only realizes the hatching problem of diapause Artemia eggs under ultraviolet light environment, but also solves the hatching problem of Artemia eggs under weak light. The problem of low hatching rate, and at the same time ensure the stability of hatching rate, the present invention is also suitable for ensuring stable hatching rate in large hatching places
The hatching solution is prepared by mixing artificial sea crystals with tap water

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water, its salinity is 20g dissolved salt / L, pH is 8.1, the concentration of dissolved oxygen is greater than 6mg / L, and the water temperature is 28°C;

[0020] The liquid surface of the hatching solution is irradiated with ultraviolet light, and the light intensity of the ultraviolet light is 800Lux.

[0021] The density of the diapause Artemia eggs in the hatching solution is 3.7 mg / L; and the Artemia eggs of Kazakhstan are used.

[0022] After 24 hours of incubation, the hatching rate of Artemia eggs reached 85%.

Embodiment 2

[0024] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water, its salinity is 20g dissolved salt / L, pH is 8.1, the concentration of dissolved oxygen is greater than 6mg / L, and the water temperature is 28°C;

[0025] The liquid surface of the hatching solution is irradiated with ultraviolet light, and the light intensity of the ultraviolet light is 800Lux.

[0026] The density of the diapause Artemia eggs in the hatching solution is 3.7 mg / L; and the shelled Artemia eggs of Kazakhstan are used.

[0027] After 24 hours of incubation, the hatching rate of Artemia eggs reached 90%.

Embodiment 3

[0029] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water. The salinity is 20g dissolved salt / L, the pH is 8.1, the concentration of dissolved oxygen is greater than 6mg / L, the water temperature is 28°C, and 90mg / L is added to the hatching solution. tea polyphenols;

[0030] The liquid surface of the hatching solution is irradiated with ultraviolet light, and the light intensity of the ultraviolet light is 800Lux.

[0031] The density of the diapause Artemia eggs in the hatching solution is 3.7 mg / L; and the shelled Artemia eggs of Kazakhstan are used.

[0032] After 24 hours of incubation, the hatching rate of Artemia eggs reached 93%.

[0033] The Kazakhstan artemia ovum of shelling treatment used in embodiment two and embodiment three all obtains through following processing steps:

[0034] 1) Conf...

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Abstract

The invention provides a method for hatching under-diapause brine shrimp eggs in ultraviolet light. According to the method, the under-diapause brine shrimp eggs are hatched in hatching liquid under the density of 3.5-4mg / L, the hatching liquid contains 19-22g of dissolved salt per L in terms of salinity, has pH of 8-9 and is greater than 6mg / L in concentration of dissolved oxygen and 27.5-28.5 DEG C in temperature, and the ultraviolet light is 500-1000Lux in luminous intensity. The hatching liquid made from aerated tap water and artificial seawater crystal. The method has the advantages that hatching the under-diapause brine shrimp eggs in the ultraviolet light is achieved, the rate of hatching the under-diapause brine shrimp eggs in the ultraviolet light can be effectively increased through controlling of the factors such as luminous intensity, temperature, pH value and dissolved oxygen, 24h hatching rate reaches greater than 85%, and the problem that the rate of hatching the brine shrimp eggs in the ultraviolet light is low is solved.

Description

technical field [0001] The invention relates to the technical field of Artemia hatching, in particular to a method for hatching diapause Artemia eggs under ultraviolet light. Background technique [0002] Artemia nauplii is generally used as live feed for aquaculture, especially as live feed for marine fish and sea shrimp larvae in the early stage. Generally, there is no such live feed for Artemia nauplii in the market, only Artemia nauplii eggs, nauplii It is hatched from Artemia eggs. The hatching of Artemia eggs can be completed in the hatching solution, but at present, people have different opinions on the salinity, pH, light intensity, and incubation time required for Artemia hatching. Most of the hatching research focuses on the above aspects. Ultraviolet light is a kind of weak light, and it is also a kind of harmful light. No one has yet proposed the influence of different energy lights on the hatching of Artemia. By studying the hatching of Artemia eggs under ultrav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 孙朦朦柴慈民王永苓
Owner TIANJIN OCEAN PAL CAROL BIOTECH
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