Application of 2,4-dichlorphenoxyacetic acid to yield improvement of DHA in schizochytrium limacinum and promotion of oil accumulation of schizochytrium limacinum

A dichlorophenoxyacetic acid and oil accumulation technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of unreported application and achieve the effect of promoting oil accumulation

Inactive Publication Date: 2015-02-18
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although phytohormones are also found in microalgae, their functions and modes of...

Method used

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  • Application of 2,4-dichlorphenoxyacetic acid to yield improvement of DHA in schizochytrium limacinum and promotion of oil accumulation of schizochytrium limacinum
  • Application of 2,4-dichlorphenoxyacetic acid to yield improvement of DHA in schizochytrium limacinum and promotion of oil accumulation of schizochytrium limacinum
  • Application of 2,4-dichlorphenoxyacetic acid to yield improvement of DHA in schizochytrium limacinum and promotion of oil accumulation of schizochytrium limacinum

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Experimental program
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Effect test

Embodiment 1

[0037] In order to investigate the effect of 2,4-D on the polyunsaturated fatty acid components of Schizochytrium sp. 680 When the value is 0.5, add 2,4-D at concentrations of 0mg / L, 0.5mg / L, 1.5mg / L, 3mg / L, 6mg / L, and 12mg / L.

[0038] Fresh Schizochytrium sp. was inoculated at an inoculum amount of 5% by volume in the culture solutions of each of the above groups, and cultured in a constant temperature light incubator at 25°C and 3000 lux until the adjustment period to obtain algae liquid. During the cultivation process, the algae liquid was taken every 24 hours, and the absorbance of the algae liquid was measured at 680 nm to obtain the growth curve of Schizochytrium sp.

[0039] The above-mentioned algae liquid is centrifugally concentrated, washed with deionized water, and centrifuged to remove the culture salt of Schizochytrium algae cells, and prepare wet algae without culture salt for oil extraction.

[0040] The effects of different concentrations of 2,4-D on the fatt...

Embodiment 2

[0045] In order to investigate the effect of 2,4-D on the oil accumulation of Schizochytrium sp., we conducted experiments in a similar manner to Example 1. The specific medium we configured was to add different concentrations of 2,4-D to the basic Tap medium. , respectively control the concentration of 2,4-D in the medium to 0mg / L, 0.5mg / L, 1.5mg / L, 3mg / L, 6mg / L, 12mg / L.

[0046] Table 2 shows the effects of different concentrations of 2,4-D on the oil accumulation of Schizochytrium during the growth adjustment period. The results showed that compared with the control group (the concentration of 2,4-D was 0 μg / L), the addition of 2,4-D during the adjustment period of Schizochytrium growth significantly increased the oil accumulation of Schizochytrium sp. The degree has a great relationship with the concentration of 2,4-D. When the concentration of 2,4-D is 1.5mg / L, the accumulation of oil in Schizochytrium alba oil reaches the highest level, which is 1.13 times that of the co...

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Abstract

The invention discloses a method for adding 2,4-dichlorphenoxyacetic acid to improve the yield of DHA (Docosahexaenoic Acid) in schizochytrium limacinum and promote the oil accumulation of schizochytrium limacinum in a schizochytrium limacinum growth process, and belongs to the technical field of microalgae biology. The method specifically comprises the following operating steps: (1) adding 2,4-dichlorphenoxyacetic acid at different concentrations in a regulation period of the growth of the schizochytrium limacinum; (2) analyzing the influence of the addition of the 2,4-dichlorphenoxyacetic acid at different concentrations on oil accumulation and DHA content. According to the method, the addition of the 2,4-dichlorphenoxyacetic acid for improving the oil accumulation of the schizochytrium limacinum and increasing the DHA content in the schizochytrium limacinum growth process is put forward for the first time, and a new idea is provided for promoting the oil accumulation of the schizochytrium limacinum and increasing the DHA content by virtue of a chemical method.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and specifically relates to the application of 2,4-dichlorophenoxyacetic acid in increasing the DHA output of Schizochytrium algae and promoting the oil accumulation of Schizochytrium algae. Background technique [0002] DHA is an omega-3 polyunsaturated fatty acid, which plays an important role in the development of the brain and retina, and has attracted widespread attention. The lack of omega-3 polyunsaturated fatty acids in the diet increases the risk of cancer, depression, Alzheimer's disease, diabetes, hypertension and cardiovascular disease, cerebrovascular accident, arthritis and atherosclerosis. Currently, fish is proven to be the main source of DHA, but statistics show that the amount of fish on the market has increased from 1 million tons in 1950 to about 20 million tons, and there has been no increase after 1990, which was subsequently confirmed to be close to The fact that th...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6472
Inventor 陈以峰何冰阿里·帕萨爱默尔
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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