DNA Barcoding-based identification kit for pelteobagrus fulvidraco, ictalurus nebulosus, weever and biological products of pelteobagrus fulvidraco, ictalurus nebulosus and weever

A technology for cloud catfish and yellow catfish, applied in the field of fish identification, can solve the problems of being unable to screen and identify fish, time-consuming, and high cost

Inactive Publication Date: 2015-03-25
GANSU AGRI UNIV
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Problems solved by technology

However, because the traditional morphological taxonomy is not paid enough attention at present, the professionals of morphological taxonomy are gradually decreasing, which affects the work of fish resource protection and habitat research.
[0003] CN200510022323.1, a rapid identification method of fish molecular biology developed by Sichuan Uni

Method used

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Examples

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Effect test

Embodiment Construction

[0017] Refer to the "Guidelines for Molecular Cloning Tests" with slight modifications: (1) Take 10 mg of muscle tissue from each sample, and use ophthalmic anatomical scissors to cut it into pieces as much as possible. (2) Carefully pack the extracted sample into a 1.5ml autoclaved centrifuge tube, and then add 600ul of SET (lysate). (3) Add 10ul of Prok (20) to each EP tube, then add 15ul of SDS, and mix well. (4) Digest overnight in a 55°C water bath to fully digest the muscle tissue. (5) Add an equal volume of about 600u of Tris-saturated phenol to each EP tube, shake and mix for 15 minutes, and then centrifuge at 1200 rpm for 10 minutes. (6) Take out the supernatant, put it into a new EP tube, add an equal volume of Tris-saturated phenol, shake and mix for 15 minutes, and centrifuge at 12,000 rpm for 10 minutes. (7) Take the supernatant, add an equal volume of phenol: chloroform: isoamyl alcohol (25:24:1) and mix for 10 minutes, then centrifuge at 12000 rpm for 10 minut...

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PUM

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Abstract

The invention discloses a DNA Barcoding-based identification method for pelteobagrus fulvidraco, ictalurus nebulosus, weever and biological products of yellow-head catfish meat, ictalurus nebulosus and weever. The three fishes and uncooked meat products of the three fishes are identified according to lengths of edited base sequences, the method is established based on DNA Barcoding and is not limited by morphology of identification materials, and accurate conclusion can be made even only individual tissue fragments are available, so that the DNA Barcoding-based identification method can be widely applied to accurate identification on fishes and uncooked meat products of fishes.

Description

technical field [0001] The invention discloses a method for identifying fish, in particular to a method for identifying yellow catfish, cloud catfish and perch based on DNA Barcoding. Background technique [0002] As an indicator organism of the aquatic environment, fish can not only be used as an indicator of water purification, but also as an indicator of alien species invasion. At the same time, fish diversity indicators can also reflect their habitat status. In recent years, with the rapid development of my country's economy, ecological problems have become increasingly prominent, which provides convenient conditions for the invasion of alien species and brings hidden worries to the development and protection of local fish. How to quickly and effectively identify fish has become an urgent problem to be solved. However, because the traditional morphological taxonomy is not paid enough attention at present, the professionals of morphological taxonomy are gradually decreas...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888
Inventor 赵生国边琳鹤汪文强李永青郭永博郑王山权金强车拢杰
Owner GANSU AGRI UNIV
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