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A method for detecting the quality of frangipani medicinal material or frangipani extract

A kind of frangipani and extract technology, applied in the directions of measuring device, material separation, material analysis, etc., can solve the problem of no report of 15-desmethyl frangipani and the like

Active Publication Date: 2015-12-09
GUANGZHOU WANGLAOJI MAJOR HEALTH IND
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Problems solved by technology

[0003] 15-desmethyl frangipani has been reported in the root bark of Plumeria frangipani (Hong Ting, et al., Research Progress on Chemical Constituents and Biological Activities in Frangipani Frangipani, Natural Product Research and Development, 2011, 23: 565-570) , but there is no report on whether it contains 15-desmethyl plumerin in the flowers

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  • A method for detecting the quality of frangipani medicinal material or frangipani extract
  • A method for detecting the quality of frangipani medicinal material or frangipani extract
  • A method for detecting the quality of frangipani medicinal material or frangipani extract

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Embodiment 1

[0016] Embodiment 1 The quality detection method of Frangipani medicinal material of the present invention

[0017] 1 Instruments and materials

[0018] Agilent 1200 high performance liquid chromatography (quaternary pump, column thermostat, VWD detector, Agilent Technologies, USA); TU-1810 ultraviolet-visible spectrophotometer (Beijing General Instrument); Sartorius BP124S electronic balance (sensitivity Quantity: 0.1mg, Germany); SartoriusBP211D electronic balance (sensitivity: 0.01mg, Germany); KQ250B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); LSF-80 high-speed pulverizer (Changde Traditional Chinese Medicine Machinery Factory) .

[0019] Commercially available frangipani medicinal materials (produced in 11 batches in Guangdong, Guangxi, Yunnan, and Hubei) were purchased, and were identified as dried flowers of Plumeria rubra L., a plant of the genus Plumeria in the Apocynaceae family, by postdoctoral fellow Zhao Bin from the School of Chinese Materia Me...

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Abstract

The invention provides a method for detecting the quality of plumeria rubra L. medicinal material or plumeria rubra L. extract. The method comprises the following steps: detecting the quality of dried flowers and extract of plumeria rubra L. of apocynaceae plumeria L. plants by virtue of an HPLC method; and determining by adopting the high performance liquid chromatography method, wherein chromatographic conditions are as follows: octadecyl bonded phase silica gel is used as a filling agent, acetonitrile-0.5% phosphoric acid water (at a ratio of 8:92) is used as a mobile phase and the detection wavelength is 210nm. The inventor researches and finds that 15-demethylated plumieride is one of iridoid glycoside components in plumeria rubra L. and the content is relatively high; and the method can be used for quality detection and control of the flowers of plumeria rubra L. According to the method provided by the invention, the content of 15-demethylated plumieride in plumeria rubra L. is determined by adopting the HPLC method for the first time, and scientific basis is provided for quality standards of plumeria rubra L.

Description

technical field [0001] The invention relates to a method for detecting the quality of frangipani medicinal material or frangipani extract. Background technique [0002] Plumeria, also known as Gardenia Burmese, Plumeria japonica, Plumeria japonica, is the dry flower of Plumeria rubra L., a plant of the genus Plumeria in the family Apocynaceae. It is native to tropical America and is now widely planted in tropical regions of the world. In my country, it is mainly produced in Guangdong, Fujian, Guangxi, Hainan and other places. It is sweet, light, and cool in nature. It has the effects of clearing heat, promoting dampness, stopping dysentery, moistening the lungs, relieving cough and detoxifying. It is a commonly used herbal medicine in the Lingnan area, and it is also one of the main raw materials of Guangdong herbal teas. For example, the famous herbal teas sold in the market "Wanglaoji herbal tea" and "Wuhua tea" all use frangipani as the main raw material. With the devel...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 赵斌邓仙梅李蓉刘敬王琼柳锐娜
Owner GUANGZHOU WANGLAOJI MAJOR HEALTH IND
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