Method for rapidly detecting gram negative and positive bacteria

Abstract

The invention discloses a method for rapidly detecting gram negative and positive bacteria. The method comprises the following steps: coupling nano magnetic beads to a lipoteichoic acid antibody or an aptamer, and sealing uncoupled sites, so as to obtain positive bacteria-enriched magnetic beads; coupling the nano magnetic beads to a lipopolysaccharide antibody or the aptamer, and sealing the uncoupled sites, so as to obtain negative bacteria-enriched magnetic beads; and respectively mixing the positive bacteria-enriched magnetic beads or the negative bacteria-enriched magnetic beads with a detection sample evenly, incubating, washing, adding a fluorescent labeling second antibody or an enzyme labeling second antibody, washing and determining the result. Through the manner, according to the method for rapidly detecting gram negative and positive bacteria, the low-concentration bacteria polluted sample can be detected; the operation time is shortened; automation is easy to achieve; the operation is simplified; the detection accuracy is ensured; quantitative determination can be achieved; the method can be widely applied to screening of bacterial pollution in the fields of medical health, food, medicines and the like; the problems of presence and absence of bacteria, the bacteria content and the like are solved; and the rapid, accurate and reliable detection requirements can be met.

Description

technical field [0001] The invention relates to the field of microorganism detection, in particular to a detection method for rapid quantitative identification of Gram-negative positive bacteria in fluid samples. Background technique [0002] Bacteria exist in various environments, including water, food, and even the human body, and are closely related to human life. Bacteria are both useful and harmful to the environment, humans and animals. The rapid detection of biological harmful bacteria can help the prevention and treatment of diseases, and research in this area is also in the ascendant. A large number of bacterial detection work is required in fields such as medical treatment, quarantine, environment, agriculture, pharmaceuticals and food processing. At present, there are more than ten kinds of common bacteria detection methods, and the methods for directly detecting bacteria all need to carry out bacterial culture or DNA amplification on the sample. [0003] Takin...

AI Technical Summary

Problems solved by technology

However, the number of bacteria in platelet products may be relatively low before culture, so that it cannot be detected; at the same time, the amount of clinical blood used in my country is huge, and the culture time is too long to effectively ensure the safe use of blood in my country; and hospitals cannot quickly transfuse blood before clinical transfusion. Testing for bloodborne bacteria could increase patients' transfusion risk

At present, the main detection methods have three problems: time-consuming, cumbersome operation, and low sensitivity

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