Msl male sterile recurrent population of EPSPS gene transgenetic soybean and construction method thereof

Abstract

The invention discloses an EPSPS gene transgenetic msl soybean population and its construction method. According to the invention, Shuang-Jin Zhao improved soybean msl male sterile recurrent base population is introduced; conventional PCR molecular biology analysis and field phenotypic identification are carried out while transgenic materials containing a herbicide-resistant gene EPSPS are determined; seeds from different varieties of EPSPS transgenetic gene materials are mixed according to the ratio of 1:1; after the mixed transgenetic seeds and seeds of the soybean msl male sterile recurrent base population are mixed according to the ratio of 1:1, the mixed seeds are planted in the field; when the soybean is ripened, seeds from sterile plants in the population are harvested; In the next year, the seeds from sterile plants are planted in the field and are sprayed with glyphosate to screen resistant strains; and during maturation, the resistant strains are mixed and harvested so as to preliminarily construct the population. According to the invention, wide hereditary basis of the msl male sterile recurrent base population and herbicide resistant characteristics of the transgenic materials are combined, and through natural crossing, multiple excellent genes can be polymerized and new multi-resistant high-quality soybean species can be cultivated. Work such as multiple combination of artificial crossing and genetic transformation of soybean EPSPS transgenetic genes are saved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to an EPSPS gene-transferred ms1 soybean population and a construction method thereof. Background technique [0002] 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) catalyzes the synthesis of aromatic amino acids (tryptophan, phenylalanine, and tyrosine) from PEP. Glyphosate is a broad-spectrum herbicide that can be combined with EPSPS as a competitive inhibitor to form a complex of EPSPS-S3P-glyphosate, thereby inhibiting the activity of EPSP synthase and blocking the synthesis of chorismate, thereby blocking the aromatic The synthesis of amino acids disturbs the normal nitrogen metabolism of organisms and eventually leads to the death of organisms. By transfecting the EPSPS gene, the expression level of the EPSPS gene can be increased, thereby obtaining herbicide-resistant plants. Since herbicide-resistant soybeans were put into commercial planting, its planting area ha...

AI Technical Summary

Problems solved by technology

[0005] The reincarnation population not only overcomes the time-consuming and labor-intensive problems caused by conventional hybridization, but also has the disadvantages of low survival rate, and enriches the genetic basis of the reincarnation population to the greatest extent.

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