Msl male sterile recurrent population of EPSPS gene transgenetic soybean and construction method thereof

A technology of male sterility and reincarnation group, applied in the field of bioengineering, it can solve the problems of time-consuming, labor-intensive and low survival rate.

Inactive Publication Date: 2015-04-01
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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Problems solved by technology

[0005] The reincarnation population not only overcomes the time-consuming and labor-intensive problems caused by conventional hybridization,

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  • Msl male sterile recurrent population of EPSPS gene transgenetic soybean and construction method thereof
  • Msl male sterile recurrent population of EPSPS gene transgenetic soybean and construction method thereof

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Embodiment Construction

[0016] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0017] 1. Research on the regularity of transgenic pollen received by ms1 population under the mixed planting method

[0018] Transgenic soybean lines Ji K32, Ji K331, Ji K69, Ji K964 and other soybeans at different maturity stages were mixed evenly at a ratio of 1:1, and then mixed with non-transgenic ms1 soybean populations at a ratio of 1:1, 2:1, 3:1, and 4, respectively. :1, 5:1 five ratios of planting. The planting area is 150 square meters. Finally, the seed resistance on the sterile plants was counted. Detection method Spray the whole soybean plant with 400ml / mu dose at the third compound leaf stage of the first soybean. The surviving seedlings were resprayed once. Finally, the survival rate was counted. The results showed that with the increase of the proportion of transgenic materials in the pop...

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Abstract

The invention discloses an EPSPS gene transgenetic msl soybean population and its construction method. According to the invention, Shuang-Jin Zhao improved soybean msl male sterile recurrent base population is introduced; conventional PCR molecular biology analysis and field phenotypic identification are carried out while transgenic materials containing a herbicide-resistant gene EPSPS are determined; seeds from different varieties of EPSPS transgenetic gene materials are mixed according to the ratio of 1:1; after the mixed transgenetic seeds and seeds of the soybean msl male sterile recurrent base population are mixed according to the ratio of 1:1, the mixed seeds are planted in the field; when the soybean is ripened, seeds from sterile plants in the population are harvested; In the next year, the seeds from sterile plants are planted in the field and are sprayed with glyphosate to screen resistant strains; and during maturation, the resistant strains are mixed and harvested so as to preliminarily construct the population. According to the invention, wide hereditary basis of the msl male sterile recurrent base population and herbicide resistant characteristics of the transgenic materials are combined, and through natural crossing, multiple excellent genes can be polymerized and new multi-resistant high-quality soybean species can be cultivated. Work such as multiple combination of artificial crossing and genetic transformation of soybean EPSPS transgenetic genes are saved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to an EPSPS gene-transferred ms1 soybean population and a construction method thereof. Background technique [0002] 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) catalyzes the synthesis of aromatic amino acids (tryptophan, phenylalanine, and tyrosine) from PEP. Glyphosate is a broad-spectrum herbicide that can be combined with EPSPS as a competitive inhibitor to form a complex of EPSPS-S3P-glyphosate, thereby inhibiting the activity of EPSP synthase and blocking the synthesis of chorismate, thereby blocking the aromatic The synthesis of amino acids disturbs the normal nitrogen metabolism of organisms and eventually leads to the death of organisms. By transfecting the EPSPS gene, the expression level of the EPSPS gene can be increased, thereby obtaining herbicide-resistant plants. Since herbicide-resistant soybeans were put into commercial planting, its planting area ha...

Claims

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Application Information

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IPC IPC(8): A01H5/00
Inventor 张孟臣赵青松杨春燕赵双进陈强闫龙
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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