Pharmaceutical composition for reducing blood glucose as well preparation method and application of pharmaceutical composition

Abstract

The invention discloses a pharmaceutical composition for reducing blood glucose as well a preparation method and application of the pharmaceutical composition. The pharmaceutical composition is prepared from a hedge bamboo extract and pharmaceutically acceptable adjuvants, wherein the hedge bamboo extract is a Japanese platanthera herb root extract which is extracted from Japanese platanthera herb root by using acetone. The pharmaceutical composition has a significant activity of reducing the blood glucose of mice with diabetes and can not affect the fasting blood glucose of normal mice markedly, and thus the pharmaceutical composition can not cause side or toxic effect such as hypoglycemia after being taken by patients and has good clinical significance.

Description

technical field

[0001] The invention belongs to the technical field of medicine, and in particular relates to a pharmaceutical composition for lowering blood sugar, a preparation method and application thereof. Background technique

[0002] With the development of society and the improvement of living standards, the prevalence of diabetes is showing a worldwide upward trend. Among them, the growth rate of patients in developing countries far exceeds that in developed countries, and the new cases will mainly be concentrated in China, the Indochina subcontinent and Africa and other developing countries. In 2013, the Diabetes Society of the Chinese Medical Association announced the results of a diabetes epidemiological survey. The prevalence of diabetes in people over 30 years old in my country reached 11.6%. The prevalence of diabetes has become a major social and public health problem faced by ethnic minorities and underdeveloped areas in both developing and developed coun...

Examples

Embodiment 1

[0016] Embodiment 1: the preparation of Guanyin bamboo extract

[0017] Weigh 500g of dry root of Orchid rhizome, crush it into granules, use 5L of acetone solution with a volume concentration of 80%, extract at 76°C for 2 hours, suction filter, and extract the filter residue once more in the same way, combine the two filtrates, and depressurize Reclaim acetone until all the acetone is recovered, freeze-dry to obtain 39.62g solid, pulverize into fine powder, and pass through a 100-mesh sieve to obtain the Avalokitesvara bamboo extract.

Embodiment 2

[0018] Example 2: Effect test of Guanyin bamboo extract on blood sugar in experimental alloxan-induced diabetic mice

[0019] SPF grade Kunming mice, body weight 20-24g, both male and female, were placed in the same laboratory for observation and feeding 3 days before the test, free to eat and drink, and the feed and drinking water of each group were exactly the same. After the test started, 10 mice (half male and half female) were randomly selected as the normal control group, and the other mice were established as the alloxan diabetic mouse model as follows: After fasting for 12 hours, weigh them, and inject them into the tail vein for immediate use. The previously prepared alloxan physiological saline solution was injected at a dose of 65 mg / kg. After 72 hours, fasted for 8 hours, and then collected 0.2 ml of blood from a capillary glass tube in the orbital venous plexus of mice. After centrifuging for 15 minutes, the serum was taken to measure the fasting blood glucose val...

Embodiment 3

[0024] Example 3: Effect test of Guanyin bamboo extract on blood sugar in normal mice

[0025] SPF grade Kunming mice, body weight 20-24g, both male and female, were placed in the same laboratory for observation and feeding 3 days before the test, free to eat and drink, and the feed and drinking water of each group were exactly the same. After the test started, all mice were randomly divided into four groups (half male and half female), 10 in each group: normal control group (distilled water 15ml / kg); positive control group (metformin 0.75g / kg); Guanyin bamboo extract high, Low dose group (0.62, 0.31 g / kg of Guanyin bamboo extract prepared in Example 1). All groups were administered intragastrically, once a day, for 10 consecutive days. Fasting for 8 hours before the last administration, and 2 hours after the administration, take 0.2ml of blood from the capillary tube of the orbital venous plexus, and centrifuge at 3500r / min for 15min after the blood coagulates to separate ou...

technical field

[0001] The invention belongs to the technical field of medicine, and in particular relates to a pharmaceutical composition for lowering blood sugar, a preparation method and application thereof. Background technique

[0002] With the development of society and the improvement of living standards, the prevalence of diabetes is showing a worldwide upward trend. Among them, the growth rate of patients in developing countries far exceeds that in developed countries, and the new cases will mainly be concentrated in China, the Indochina subcontinent and Africa and other developing countries. In 2013, the Diabetes Society of the Chinese Medical Association announced the results of a diabetes epidemiological survey. The prevalence of diabetes in people over 30 years old in my country reached 11.6%. The prevalence of diabetes has become a major social and public health problem faced by ethnic minorities and underdeveloped areas in both developing and developed coun...

Examples

Embodiment 1

[0016] Embodiment 1: the preparation of Guanyin bamboo extract

[0017] Weigh 500g of dry root of Orchid rhizome, crush it into granules, use 5L of acetone solution with a volume concentration of 80%, extract at 76°C for 2 hours, suction filter, and extract the filter residue once more in the same way, combine the two filtrates, and depressurize Reclaim acetone until all the acetone is recovered, freeze-dry to obtain 39.62g solid, pulverize into fine powder, and pass through a 100-mesh sieve to obtain the Avalokitesvara bamboo extract.

Embodiment 2

[0018] Example 2: Effect test of Guanyin bamboo extract on blood sugar in experimental alloxan-induced diabetic mice

[0019] SPF grade Kunming mice, body weight 20-24g, both male and female, were placed in the same laboratory for observation and feeding 3 days before the test, free to eat and drink, and the feed and drinking water of each group were exactly the same. After the test started, 10 mice (half male and half female) were randomly selected as the normal control group, and the other mice were established as the alloxan diabetic mouse model as follows: After fasting for 12 hours, weigh them, and inject them into the tail vein for immediate use. The previously prepared alloxan physiological saline solution was injected at a dose of 65 mg / kg. After 72 hours, fasted for 8 hours, and then collected 0.2 ml of blood from a capillary glass tube in the orbital venous plexus of mice. After centrifuging for 15 minutes, the serum was taken to measure the fasting blood glucose val...

Embodiment 3

[0024] Example 3: Effect test of Guanyin bamboo extract on blood sugar in normal mice

[0025] SPF grade Kunming mice, body weight 20-24g, both male and female, were placed in the same laboratory for observation and feeding 3 days before the test, free to eat and drink, and the feed and drinking water of each group were exactly the same. After the test started, all mice were randomly divided into four groups (half male and half female), 10 in each group: normal control group (distilled water 15ml / kg); positive control group (metformin 0.75g / kg); Guanyin bamboo extract high, Low dose group (0.62, 0.31 g / kg of Guanyin bamboo extract prepared in Example 1). All groups were administered intragastrically, once a day, for 10 consecutive days. Fasting for 8 hours before the last administration, and 2 hours after the administration, take 0.2ml of blood from the capillary tube of the orbital venous plexus, and centrifuge at 3500r / min for 15min after the blood coagulates to separate ou...

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