Primers for differential diagnosis of porcine epidemic diarrhea virus virulent strain and attenuated strain and detection kit thereof

Abstract

The invention relates to primers for differential diagnosis of a porcine epidemic diarrhea virus virulent strain and attenuated strain and a detection kit thereof, and belongs to the field of biotechnology. Total RNA is used as a template, the detection primers for differential diagnosis of the porcine epidemic diarrhea virus virulent strain and attenuated strain are used for RT-PCR amplification, and after the reaction is finished, a result is judged according to the amplified fragment number and the amplified fragment molecular weight range. The primers have good specificity, the detection method is fast and simple, the accuracy is high, and a guarantee is provided for the differential diagnosis of the porcine epidemic diarrhea virus virulent strain and attenuated strain.

Description

technical field

[0001] The invention relates to primers for differential diagnosis of strong and weak strains of porcine epidemic diarrhea virus and a detection kit thereof, belonging to the field of biotechnology. Background technique

[0002] Porcine epidemic diarrhea virus (PEDV) is a member of the Coronaviridae family. Clinically, PEDV can cause porcine epidemic diarrhea (porcine epidemic diarrhea, PED), which is characterized by diarrhea, vomiting, dehydration and high lethality in suckling piglets. PED was first reported in Belgium and the UK in 1978 (Pensaert M B, de Bouck P. A new coronavirus—like particle associated with diarrhea in swine. Arch Virol, 1978, 58: 243-247). Its genome is a single-molecule linear positive-sense single-stranded RNA with a size of about 28kb. Its 5' end is capped and its 3' end is a polyA tail, which is infectious. The genome contains 5'-UTR, 3'-UTR and at least 7 ORFs. Four of these seven ORFs encode structural proteins: spike protein...

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