Spawning device for small brown planthoppers and application method thereof

Abstract

The invention relates to a spawning device for small brown planthoppers, which is used for detecting the reproductive capacity of the small brown planthoppers in an RNA (Ribose Nucleic Acid) interference test. The device is concretely composed of: a glass tube with openings at two ends, 80-mesh nylon yarn, a rubber band, sterile toothpicks, wetting sterile degreasing cotton and a sealing film (Parafilm). During use, use the 80-mesh nylon yarn to seal one end of the glass tube; then take a toothpick wound by the wetting sterile degreasing cotton, use the sealing film to seal the degreasing cotton, and imitate rice seedlings; put in adult small brown planthoppers to lay eggs, use a layer of sealing film to seal the other end of the glass tube; add artificial feed in the center of the film, then reuse a layer of sealing film to seal the feed; after completion, use a humidifier to conduct mist spray for preserving moisture. The device can solve the problem that the small brown planthoppers do not have a spawning site in the RNAi study of a feeding method, and provides guarantee for successfully carrying out the gene function study.

Description

1. Technical field [0001] The invention relates to an oviposition device of the small planthopper, which is used for detecting the fecundity of the small planthopper when the gene function of the small planthopper is studied by the RNA interference method. 2. Background technology [0002] The small brown planthopper, Laodelphax striatellus Fallèn, is one of the important rice pests in my country and occurs in all rice areas. In addition to being affected by stress factors such as temperature and pesticides, fecundity is the decisive factor affecting its population size. Exploring the functions of reproduction-related genes can further elucidate the molecular basis of SBPH's fecundity changes, and provide target resources for the use of biological techniques to control SBPH. [0003] RNA interference (RNA interference, RNAi) refers to the phenomenon of efficient and specific degradation of homologous mRNA induced by double-stranded RNA, which is highly conserved during evol...

AI Technical Summary

Problems solved by technology

But for fecundity, it is necessary to check the number of eggs laid by the test insects throughout the adult period from emergence to death, which leads to a contradiction

Because SBPH normally lays eggs in rice stalks, if rice stalks are placed in RNAi, SBPH will choose to eat rice stalks instead of feed, so that dsRNA cannot be taken in, resulting in RNAi failure; Putting rice stalks, the small planthopper will face the situation of having nowhere to lay eggs

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