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Method for extracting and separating bromelain from pineapple peels with reverse micelle having Gemini surfactant

A technology of surfactants and reverse micelles, applied in biochemical equipment and methods, enzymes, peptidases, etc., can solve the problems of environmental pollution, waste of resources, etc., achieve the effects of low dosage, reduced pollution, and increased utilization value

Inactive Publication Date: 2015-04-22
YANGZHOU UNIV
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AI Technical Summary

Benefits of technology

This technology allows for efficient extracting from nuts without losing their nutritional content or causing harmful chemicals that may be released into our environment during processing. It achieves this by reducing the use of excessive amounts of detergent solvent while maintaining good quality products with minimal damage caused through evaporation over time.

Problems solved by technology

The technical problem addressed by this patents relates to improving the efficiency at producing or purifying biological molecules like bromelin while reducing environmental impact during manufacturing procedures that involve extracting them out of their original source material (PSP).

Method used

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  • Method for extracting and separating bromelain from pineapple peels with reverse micelle having Gemini surfactant
  • Method for extracting and separating bromelain from pineapple peels with reverse micelle having Gemini surfactant
  • Method for extracting and separating bromelain from pineapple peels with reverse micelle having Gemini surfactant

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Embodiment Construction

[0026] 1. Specific preparation process:

[0027] 1. Reverse micelles preparation:

[0028] Dissolve 3-90g of quaternary ammonium salt Gemini surfactant in 1 L of non-polar solvent, then add 1-100mL of water, shake fully until the solution is clear and transparent, that is, reverse micelles.

[0029] In this way, the proportion of quaternary ammonium salt Gemini surfactant in each reverse micelle is 0.003g / ml~0.05g / ml, and the above non-polar solvent is composed of n-hexane and n-hexanol, and n-hexane and n-hexanol The volume ratio of the water and the quaternary ammonium salt Gemini surfactant is 5-20:1.

[0030] 2. Preparation of Bromelain Crude Extract:

[0031] Preparation of buffer solution: a phosphate buffer solution with a pH range of 5.5-6.5 was prepared using disodium hydrogen phosphate and sodium dihydrogen phosphate in a conventional method.

[0032] Wash the pineapple peel, drain it, cut it into small pieces and store it in a freezer at -20°C. Take a certain am...

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Abstract

The invention relates to a method for extracting and separating bromelain from pineapple peels with a reverse micelle having a Gemini surfactant and belongs to the technical field of reverse micelle extraction in biological engineering. According to the method, bromelain is extracted from the pineapple peels with reverse micelle having the quaternary ammonium salt Gemini surfactant; during extraction, a small amount of the surfactant is only needed, bromelain in the pineapple peel crude extraction liquid can be transferred to the reverse micelle by controlling the pH of forward-extraction liquid and the pollution of the surfactant to bromelain can be decreased; and by adjusting the ratio of n-hexane to hexanol, the activity of bromelain can also be increased. The method is simple in operation process, the activity loss of bromelain is low, the activity recovery rate of bromelain is higher, the activity recovery rate of bromelain obtained by extraction can reach 163%, and the purification factor can reach 4.

Description

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Claims

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Application Information

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Owner YANGZHOU UNIV
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