Primer and kit for distinguishing and detecting low-pathogenicity and high-pathogenicity babesia motasi

Abstract

The invention discloses a primer and a detecting kit for distinguishing and detecting low-pathogenicity and high-pathogenicity babesia motasi. The primers in the primer sequence and the kit disclosed by the invention are respectively primers in SEQ ID No.1, SEQ ID No.2, SEQ ID No.4 and SEQ ID No.5, and probe primers SEQ ID No.3 and SEQ ID No.6. Through using the primer and the kit disclosed by the invention for detection, the advantages of high sensitivity, high speed, high specificity and the like are achieved, and the primer and the kit disclosed by the invention can be widely applied to the qualitative and quantitative detection of pathogens.

Description

technical field [0001] The invention relates to a primer pair and a kit for detecting animal parasites. Specifically, the invention relates to a primer and a detection kit that can distinguish between low pathogenicity and high pathogenicity Babesia mosoni. Background technique [0002] There are more than 100 species of Babesia reported, among which Babesia ovis is named and recognized to infect sheep. B.ovis ), Babesia mosoni ( B. motasi ), Babesia rougha ( B. crassi ) and some undetermined species of Babesia. Babesia ovis from the genus Rhizocephalus ( Rhipicephalus ) is a highly pathogenic small Babesia whose biological characteristics are basically the same as Babesia bovis; Babesia crassa is a large Babesia with low pathogenicity, with four divisions or It reproduces by binary fission, and its transmission medium ticks and mode of transmission are not clear, and it is only found in Iran and Turkey; while Babesia mosoni is the most widely distributed and most co...

AI Technical Summary

Problems solved by technology

[0006] The gold standard for the detection of Babesia is the blood smear staining microscopic examination, but the results of the blood smear staining microscopic examination are affected by many factors, the detection rate is low, and the accuracy is poor

Although nucleic acid methods such as conventional PCR, nested PCR, and real-time fluorescent PCR based on SYBR Green dye are applied, conventional PCR has disadvantages such as low sensitivity, poor specificity, and long detection cycle; nested PCR has high sensitivity, but the two-step PCR reaction cumbersome and easily polluted, not suitable for large-scale epidemiological investigations

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