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Analysis and identification method for flavonoid constituent in callicarpa nudiflora

A technique for identification and identification of Auranthus nudiflora, which is applied in the field of rapid analysis and identification of various flavonoids in Aurora nudiflora, which can solve problems such as incompleteness and limited methods, and achieve time-consuming solutions, avoid duplication of work, and ensure product quality Effect

Active Publication Date: 2015-05-20
JIUZHITANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the content of total flavonoids is mainly determined by ultraviolet spectrophotometry, but this method is very limited and not comprehensive enough; in the existing research on the chemical components of Auranthus nudiflora, there is no separate analysis and identification of flavonoids

Method used

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  • Analysis and identification method for flavonoid constituent in callicarpa nudiflora
  • Analysis and identification method for flavonoid constituent in callicarpa nudiflora
  • Analysis and identification method for flavonoid constituent in callicarpa nudiflora

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Sample preparation

[0026] Take 1kg of Nasturtina chinensis medicinal material, add 15 times the amount of water to soak for 30 minutes, boil and extract for 2 hours, filter, add 15 times the amount of water to boil and extract the medicinal residue for 2 hours, combine the two filtrates, and concentrate under reduced pressure to a relative density of 1.2 (60 ° C) ; Accurately weigh 2g into a centrifuge tube, add 7ml of methanol, mix for 2min, ultrasonically extract for 30min, centrifuge for 5min, the speed is 10000rad / min, take the supernatant, add 2g of polyamide powder, and evaporate in a water bath; The powder was placed in a G3 sand core funnel, washed 4 times with pure water, 10 ml each time, and then washed twice with 20% methanol, 10 ml each time, discarded the washing solution, and eluted with 70% methanol in 3 times, adding the first two times. 10ml of methanol, 5ml of methanol was added for the third time, 70% methanol was added to the eluent and the vol...

Embodiment 2

[0036] (1) Sample preparation

[0037] Take 1 kg of the medicinal material of Nasturtium vulgaris, add 15 times the amount of water to soak for 1 hour, boil and extract for 1 hour, filter, add 15 times the amount of water to boil and extract the medicinal residue for 1 hour, combine the two filtrates, and concentrate under reduced pressure to a relative density of 1.1 (60 ° C) ; Accurately weigh 2.5g into a centrifuge tube, add 10ml of methanol, mix for 2min, ultrasonically extract for 2h, centrifuge for 5min at a speed of 8000rad / min, take the supernatant, add 2.5g of polyamide powder, and evaporate to dryness in a water bath; The polyamide powder was placed in a G3 sand core funnel, washed twice with pure water, 5ml each time, and then washed three times with 20% methanol, each 5ml, discarded the washing solution, and eluted with 70% methanol in 3 times. Add 8 ml of methanol for the first time, add 5 ml of methanol for the third time, add 70% methanol to the eluent to make u...

Embodiment 3

[0047] (1) Sample preparation

[0048] Take 1kg of the medicinal material of Nasturtium vulgaris, add 15 times the amount of water to soak for 2 hours, boil and extract for 2 hours, filter, add 15 times the amount of water to boil and extract the medicinal residue for 2 hours, combine the two filtrates, and concentrate under reduced pressure to a relative density of 1.3 (60 ° C) ; Accurately weigh 2g into a centrifuge tube, add 10ml of methanol, mix for 2min, ultrasonically extract for 2h, centrifuge for 5min at a speed of 10000rad / min, take the supernatant and add 2g of polyamide powder, and evaporate to dryness in a water bath; The powder is placed in a G3 sand core funnel, washed 4 times with pure water, 20 ml each time, and then washed 2 times with 30% methanol, 20 ml each time, discard the washing solution, and elute with 70% methanol in 3 times, adding the first two times. 10 ml of methanol, 3 ml of methanol was added for the third time, 70% methanol was added to the elu...

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Abstract

The invention relates to an analysis and identification method for flavonoid constituent in callicarpa nudiflora. The invention establishes a method for fast analyzing and identifying the flavonoid constituent in the callicarpa nudiflora by adopting an LC-MS / MS method, and thus a feasible base for the quality control of a callicarpa nudiflora preparation is provided.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, in particular to a method for rapid analysis and identification of various flavonoid components in Viola nudiflora, which provides a feasible basis for the quality control of Viola nudiflora preparations. Background technique [0002] Callicarpa nudiflora Hook.et Arn. is a plant of the Verbenaceae family, mainly produced in Guangdong, Hainan, Guangxi and other places. It has the functions of stopping bleeding, removing blood stasis and relieving pain. It is mainly used for pulmonary hemoptysis, gastrointestinal bleeding, epistaxis, bleeding gums, traumatic bleeding, traumatic injury, rheumatism, swelling and pain, etc. The main components of Nasturtium are flavonoids and tannins. At present, the content of total flavonoids is mainly determined by ultraviolet spectrophotometry, but this method is very limited and not comprehensive; in the existing research on the chemical constituents...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 颜冬兰谷陟欣朱丽任洁芳张妮瑜
Owner JIUZHITANG
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