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Reagent, kit and test paper for determining concentration of oxalic acid, and preparation method for test paper

A concentration, oxalic acid technology, applied in material analysis by observing the effect of chemical indicators, analysis by chemical reaction of materials, electrochemical variables of materials, etc. , difficult operation and other problems, to achieve the effect of short test time, high sensitivity and complex operation

Inactive Publication Date: 2015-06-03
WUHAN KANGFUDE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows us to detect substances such as oxyacids quickly and easily from samples that may contain other compounds like nitrogen monosulfurous amines. It uses different methods than existing techniques but they work better together due to their unique properties compared to previous technologies.

Problems solved by technology

Technological Problem addressed in this patents relates to improving the accuracy and effectiveness of measuring oxalatosis levels in humans due to its unique characteristics. Current methods involve invasively obtaining blood values containing these substances, but they also suffer limitations like costliness, complexity, difficulty in preparation, lack of standardization, and potential harmful side effects associated with current methods.

Method used

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  • Reagent, kit and test paper for determining concentration of oxalic acid, and preparation method for test paper
  • Reagent, kit and test paper for determining concentration of oxalic acid, and preparation method for test paper
  • Reagent, kit and test paper for determining concentration of oxalic acid, and preparation method for test paper

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preparation example Construction

[0041] The embodiment of the present invention also provides the above-mentioned preparation method of the electrochemical test paper for measuring the concentration of oxalic acid, comprising the following steps:

[0042] Print the working electrode lead, the reference electrode lead and the test instrument connecting lead on the substrate; coat the reference electrode and the working electrode with gelatin or polyvinyl alcohol with a concentration of 30-100g / L to form an adhesive layer; The layer is coated with benzoquinone or potassium ferricyanide with a concentration of 10-200mmol / L to make an oxidant layer; 10-2000U / L is coated on the oxidant layer, and the optimum pH is 4.0-7.5 oxalate oxidase and concentration It is 100-1000U / L horseradish peroxidase to make a reaction layer; coat a layer of hydrophilic film on the upper surface of the reaction layer, place it to dry at 37°C, and then place the substrate according to actual needs. Cut into several parts to obtain elect...

Embodiment 1

[0054] In this embodiment, the reagents used to determine the oxalic acid concentration include oxalate oxidase with a concentration of 10 U / L and an optimum pH value of 5.7, horseradish peroxidase with a concentration of 500 U / L and benzene with a concentration of 200 mmol / L Quinone.

[0055] Print the working electrode lead, the reference electrode lead, and the connecting lead of the test instrument on the substrate made of PVC (Polyvinyl chloride, polyvinyl chloride); coat the reference electrode and the working electrode with gelatin or polyvinyl alcohol with a concentration of 30g / L , make an adhesive layer; coat benzoquinone with a concentration of 200 mmol / L on the adhesive layer to make an oxidizing agent layer; coat 10 U / L on the oxidizing agent layer, the optimum pH value is 5.7 oxalate oxidase and the concentration is 500U / L of horseradish peroxidase to make a reaction layer; coat a layer of hydrophilic film on the upper surface of the reaction layer, place it to d...

Embodiment 2

[0057] In this embodiment, the reagents used to measure the oxalic acid concentration include oxalate oxidase with a concentration of 800 U / L and an optimum pH value of 6.0, horseradish peroxidase with a concentration of 2000 U / L and benzene with a concentration of 10 mmol / L Quinone.

[0058] Print the working electrode lead, the reference electrode lead, and the test instrument connecting lead on the substrate; coat the reference electrode and the working electrode with gelatin or polyvinyl alcohol with a concentration of 70g / L to form an adhesive layer; on the adhesive layer Coating the benzoquinone with a concentration of 10 mmol / L to make an oxidizing agent layer; coating 800 U / L on the oxidizing agent layer, the optimum pH value is 6.0 oxalate oxidase and the horseradish peroxidase with a concentration of 2000 U / L, making the preparation. Form a reaction layer; coat a layer of hydrophilic film on the upper surface of the reaction layer, place it to dry at a temperature of...

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Abstract

The invention discloses a reagent, a kit and a piece of test paper for determining the concentration of oxalic acid, and a preparation method for the test paper, and relates to the field of early diagnosis and prevention of kidney stone. The reagent comprises oxalate oxidase with the concentration of 10 to 2,000 U/L and the optimal pH value of 4.0 to 7.5, horseradish peroxidase with the concentration of 100 to 2,000 U/L, and a reactant with the concentration of 10 to 200 mmol/L, wherein the reactant is an oxidant or a developing agent; the oxidant is benzoquinone or potassium ferricyanide; the developing agent is 3-methyl-benzothiazolinone hydrazone hydrochloride or sodium 3,5-dichloro-2-hydroxybenzenesulfonate. Electrochemical test paper and light reflecting test paper disclosed by the invention are convenient to use, easy to operate, short in determination time, high in accuracy and anti-jamming capability, and long in storage time, and can be used in a hospital, a clinic or a family.

Description

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Claims

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Application Information

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Owner WUHAN KANGFUDE BIOTECH CO LTD
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