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Application of cox‑2 in preparation of diagnostic reagents for diagnosis and detection of canine tumors

A diagnostic reagent, 1.COX-2 technology, applied in the field of diagnostic reagents for detection of canine tumors, preparation of diagnostics, can solve the problem of no research on COX-2 and canine tumors

Inactive Publication Date: 2017-06-16
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no domestic research on the relationship between COX-2 and canine tumors

Method used

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Examples

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Embodiment 1

[0023] Example 1 Detection example of PAD1 as a tumor marker

[0024] Collect 46 cases of postoperative tumor tissue and 3 normal canine mammary gland, skin, testis, perianal, and uterine tissues dissected in the Animal Hospital Affiliated to Zhejiang University, remove the fatty part of the parenchymal tissue, and cut with surgical scissors and tweezers. Divide into size approximately 1cm 3 Small tissue pieces around are easily fixed with 10% neutral formalin. All tumors were observed by pathological tissue sections, and there were 25 cases of breast tumors, 11 cases of skin and soft tissue tumors, 4 cases of perianal tumors, 2 cases of testicular tumors, and 4 cases of vaginal and uterine tumors. Among breast tumors, there were 5 cases of compound carcinoma, 3 cases of ductal papillary carcinoma, 5 cases of solid carcinoma, 1 case of undifferentiated carcinoma, 1 case of mucinous carcinoma, 3 cases of fibrosarcoma, 1 case of osteosarcoma, 3 cases of compound adenoma, fibros...

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Abstract

The invention discloses an application of COX-2 (cyclo-oxygen-ase-2) in preparation of a diagnostic reagent for dog tumor diagnosis and detection. The experiment research shows that the positive expression rate of COX-2 in dog tumors such as a breast tumor, skin and soft-tissue tumors, a perianal tumor, a testicular tumor and vagina and uterine tumors is 76.1%, the positive expression rate of COX-2 in normal tissue is only 20%, so that COX-2 can be applied as a dog tumor marker, the expression condition of COX-2 is detected with an immunohistochemical method, and a result can be taken as one of evidence items for clinically diagnosing whether a person suffers from the dog tumor. The invention further discloses a kit for detecting COX-2 and a detection method. The kit contains a 3% H2O2 solution, a citrate buffer solution, a PBS (poly butylenes succinate) buffer solution, a mouse COX-2 monoclonal antibody, a goat anti-mouse IgG antibody, a DAB (diaminobenzidine) developing solution and distilled water. The detection method comprises the step of streptaridin-peroxidase dyeing.

Description

technical field [0001] The invention relates to the application of COX-2 as a tumor marker in the preparation of diagnostic reagents for diagnosing and detecting canine tumors. Background technique [0002] Cyclo-oxygen-ase (COX) is an essential enzyme for the synthesis of prostaglandins (PGs), and it is also a key rate-limiting enzyme in the initial step of PGs synthesis. Also known as epoxide hydrase. COX has two isoenzymes COX-1 and COX-2. The former is a structural type called element enzyme or housekeeping enzyme, which mainly exists in blood vessels, stomach, kidney and other tissues. Scholars at home and abroad generally believe that the PG produced by it participates in the normal physiological process and protective functions of the body, such as maintaining the integrity of the gastrointestinal mucosa The latter is inducible, which is an inducible enzyme that is rapidly produced by stimulation, that is, its production is induced by various damaging chemical, phys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/573
CPCG01N33/573G01N33/57484
Inventor 朱国
Owner NANJING AGRICULTURAL UNIVERSITY
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