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Immobilized breeding method of microalgae

The technology of immobilized culture and microalgae culture solution is applied in the field of immobilized culture of microalgae, which can solve the problems of poor supply of culture solution, influence on the output of immobilized culture of microalgae, large dissolved gas concentration, etc., so as to improve the immobilization The effect of aquaculture production

Active Publication Date: 2015-06-24
ENN SCI & TECH DEV
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  • Abstract
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Problems solved by technology

[0004] However, the microalgae culture solution produces oxygen due to the photosynthesis of the microalgae in the immobilized cultivation device, and the microalgae culture solution is in contact with the air so that the gas is easily dissolved in the microalgae culture solution, so that the microalgae culture solution The dissolved gas concentration in the medium is relatively large. When the microalgae culture solution is supplied to the immobilized culture layer, there will be a gap between the infusion layer and the substrate, between the infusion layer and the carrier, and between the carrier and the microalgal cell layer. Bubbling occurs due to the presence of a large amount of gas, which makes the algae cells separate from the carrier, and the supply of the culture solution is not smooth, which affects the immobilized culture of microalgae.

Method used

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  • Immobilized breeding method of microalgae

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specific Embodiment approach

[0061] Hereinafter, the embodiments of the present invention illustrate the present invention through comparative examples, examples and experimental examples. These examples are merely examples for specifically illustrating the present invention, and those skilled in the art will understand that the scope of the present invention is not limited by these examples, comparative examples, and test examples.

[0062] In order to objectively evaluate the technical effect of the described embodiment, the type of microalgae used in the comparative example and the embodiment is Chlorella reinhardtii, and the microalgae culture solution is BG11 microalgae cultured with various nutrients such as nitrogen and phosphorus. The structure, orientation and placement angle of the immobilized culture device are the same, so that the light intensity and time of the immobilized culture surface are the same.

Embodiment 1

[0066] The culture method adopted in the embodiment 1 is as follows: place the immobilized culture device inoculated with Chlorella reinhardt outdoors at an angle of 50 degrees with the ground, and receive light continuously from 7:00 to 17 days. The area of ​​the immobilized culture surface is 0.1 square meters, and yeast microorganisms are added on the immobilized culture surface of the microalgae, so that the concentration ratio of the yeast microorganisms and microalgae in the initial stage of cultivation is 1:10, and culture Liquid replaces BG11 microalgae culture liquid, wherein, the concentration of glucose is less than or equal to 30g / L, conveys described Cha's culture liquid to described immobilized culture surface, carries out mixed culture to described microalgae and yeast microorganism.

[0067] During the breeding process, by measuring the oxygen concentration in the culture solution with an oxygen electrode, the glucose addition rate is adjusted according to the l...

Embodiment 2

[0070]The culture method adopted in the embodiment 2 is as follows: place the immobilized culture device inoculated with Chlorella reinhardt outdoors, fix it at an angle of 50 degrees with the ground, and receive light continuously from 7:00 to 17:00. The area of ​​the immobilized culture surface is 0.1 square meters, and the BG11 microalgae culture solution identical to the control example is added in a closed container, such as in a closed iron tank, and the oxygen dissolved concentration in the BG11 microalgae culture solution passes through the The electrode measurement is 40mg / L microalgae culture solution, and the pressure in the airtight iron tank is reduced to -0.05--0.02MPa by decompressing and pumping the airtight iron tank. The dissolved oxygen concentration in the microalgae culture solution is 2 mg / L microalgae culture solution, and the dissolved oxygen concentration is 2 mg / L. The microalgae culture solution is transported to the immobilized culture surface throug...

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Abstract

The invention relates to the field of microalgae breeding, and particularly relates to an immobilized breeding method of microalgae. A bubbling phenomenon in the process of immobilized breeding can be reduced, so that the immobilized breeding yield of microalgae is improved. The defect that in the prior art, the gas dissolved concentration or gas release of microalgae culture liquid in the process of immobilized breeding is large, so that a bubbling phenomenon easily occurs between a baseplate and a liquid conveying layer, between the liquid conveying layer and a carrier and between the carrier and a microalgae cell layer, thereby causing that the supply of the microalgae culture liquid is obstructed and the immobilized breeding yield of microalgae is low is overcome. An embodiment of the invention provides an immobilized breeding method of microalgae, which is used for carrying out immobilized breeding on microalgae by using pretreated microalgae culture liquid, wherein the gas dissolved quantity or gas release of the pretreated microalgae culture liquid in comparison with microalgae culture liquid before being pretreated is reduced.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to a method for immobilized cultivation of microalgae. Background technique [0002] Microalgae cells contain a variety of high-value nutrients and chemical raw materials. Cell metabolism produces polysaccharides, oils, proteins, pigments, etc., which are widely used in the pharmaceutical industry, food industry, environmental testing and purification, biotechnology and renewable energy manufacturing Therefore, how to improve the large-scale cultivation of microalgae has become the focus of research in various fields. [0003] At present, the culture of microalgae is mainly realized by open pond or reactor system, mainly for free culture. This method is not conducive to the large-scale culture of microalgae. It consumes a lot of water and energy, which has become a constraint to obtain high-yield microalgae. Factors, immobilized culture technology is an emerging culture techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 张凯冯倩
Owner ENN SCI & TECH DEV
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