Immobilized cultivation device, harvesting device and immobilized cultivation method

A technology of immobilized breeding and culturing device, applied in the field of immobilized breeding and immobilized breeding device, can solve the problems of cell shedding, affecting the output of immobilized breeding and uneven distribution, etc.

Inactive Publication Date: 2015-06-03
ENN SCI & TECH DEV
View PDF9 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the fixation methods of immobilized culture mainly include adsorption method, embedding method, cross-linking method, etc. In the prior art, cells or microorganisms are attached to the carrier through the fixation method, and are continuously transported to the cells attached to the carrier. However, if things go on like

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immobilized cultivation device, harvesting device and immobilized cultivation method
  • Immobilized cultivation device, harvesting device and immobilized cultivation method
  • Immobilized cultivation device, harvesting device and immobilized cultivation method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0099] Example 1

[0100] The hollow tubular body used in Example 1 is made of polypropylene material, the outer diameter of the polypropylene hollow tubular body is 2 mm, the inner diameter is 1 mm, and the pore diameter of the polypropylene hollow tubular body is 0.005-0.01 Micron, the microalgae used for inoculation is Pseudochlorococcus with a concentration of 10% algal liquid, and the cell diameter is 2-6 mm.

[0101] 1. Inoculation:

[0102] Put the polypropylene hollow tubular body 1 into the algae liquid pool with the algae liquid concentration of 10%, see Figure 5 , open the decompression pump 8 and the third valve 43, so that the pressure in the polypropylene hollow tubular body 1 is 0.1-0.6 MPa, and the culture solution in the algae pond enters through the pores on the polypropylene hollow tubular body 1. The polypropylene hollow tubular body 1 is evacuated by the decompression pump 8, and the algal cells in the algae liquid pool are blocked outside the polypropy...

Example Embodiment

[0109] Example 2

[0110] The hollow tubular body used in Example 2 is made of ceramic material through a sintering process, the pore diameter is in the range of 0.01-0.5 microns, and the microalgae used for inoculation are Pseudo-Nicochlorous algae with an algal liquid concentration of 10%, Cells are 2-6 mm in diameter.

[0111] 1. Inoculation:

[0112] Put the ceramic hollow tubular body 1 into the algae liquid pool with the algae liquid concentration of 10%, see Figure 5 , open the decompression pump 8 and the third valve 43, so that the pressure in the ceramic hollow tubular body is 0.1-0.6MPa, and the culture solution in the algae pond enters the ceramic hollow through the pores on the ceramic hollow tubular body 1. Inside the tubular body 1, and is evacuated by the decompression pump 8, and the algal cells in the algae liquid pool are blocked outside the ceramic hollow tubular body 1, attached to the immobilized culture surface, and the thickness of the algal cells af...

Example Embodiment

[0119] Experimental example:

[0120] In order to objectively evaluate the cell shedding and microalgae cultivation of the Examples 1-2, the specific experiments will be described in detail below.

[0121] 1. Test sample

[0122] The culture solution that fell into the collection tank 5 and the microalgae after the harvesting was completed during the immobilized cultivation process in the control example and Example 1-2.

[0123] 2. Test analysis method:

[0124] The OD value at the wavelength of 750nm was measured by a spectrophotometer, that is, the concentration of algal fluid.

[0125] 3. Experimental results:

[0126] Referring to Table 1, it is the OD value of the algal liquid concentration of the culture liquid flowing through the immobilized cultivation surface during the immobilized cultivation process of the control example and Examples 1-2.

[0127] Table 1

[0128]

[0129] Referring to Table 1, we found that: when the immobilized cultivation devic...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of immobilized cultivation, and particularly relates to an immobilized cultivation device, a harvesting device and an immobilized cultivation method. The nutrient solution is supplied evenly, cell detachment is reduced and the cultivation yield is increased. The defects of the prior art that the nutrient solution is supplied directly to flush the cultivation surface and easily cause the cell detachment and the nutrient solution is unevenly supplied through the nutrient solution supply mode can be overcome. The immobilized cultivation device comprises a storage device and a cultivation device, wherein the storage device is used for providing microalgae nutrient solution; the cultivation device comprises at least one hollow tubular body, the outer surface of the hollow tubular body is a microalgae immobilized cultivation surface, the hollow tubular body is communicated with the storage device, pores are formed in the hollow tubular body, and nutrient solution flowing through the interior of the hollow tubular body is penetrated through the immobilized cultivation surface through the pores.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to an immobilized cultivation device, a harvesting device and an immobilized cultivation method. Background technique [0002] Immobilized culture technology is an emerging culture technology that can be applied to large-scale culture of various microorganisms. Immobilized culture refers to the process of immobilizing free cells or microorganisms on a certain carrier, making them highly dense and maintaining biological activity, and rapidly proliferating under suitable conditions. The concentration of microorganisms and the ability of microorganisms to resist unfavorable environments can be improved through immobilized cultivation, and solid-liquid separation is facilitated after cultivation is completed, which can shorten the harvesting time. [0003] At present, the fixation methods of immobilized culture mainly include adsorption method, embedding method, cross-linking meth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M1/00C12N1/12C12R1/89
CPCC12M21/02C12M25/10C12M29/18C12M47/02C12N1/12
Inventor 张凯
Owner ENN SCI & TECH DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap