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Immobilized cultivation device, harvesting device and immobilized cultivation method

A technology of immobilized breeding and culturing device, applied in the field of immobilized breeding and immobilized breeding device, can solve the problems of cell shedding, affecting the output of immobilized breeding and uneven distribution, etc.

Inactive Publication Date: 2015-06-03
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the fixation methods of immobilized culture mainly include adsorption method, embedding method, cross-linking method, etc. In the prior art, cells or microorganisms are attached to the carrier through the fixation method, and are continuously transported to the cells attached to the carrier. However, if things go on like this, the cells or microorganisms attached to the carrier are continuously washed by the flowing culture solution, causing the cells to fall off, and the supply process of the culture solution causes the culture solution to be trapped between the attached cells or microorganisms. Uneven distribution affects the output of immobilized farming

Method used

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  • Immobilized cultivation device, harvesting device and immobilized cultivation method
  • Immobilized cultivation device, harvesting device and immobilized cultivation method
  • Immobilized cultivation device, harvesting device and immobilized cultivation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] The hollow tubular body used in Example 1 is made of polypropylene material, the outer diameter of the polypropylene hollow tubular body is 2 mm, the inner diameter is 1 mm, and the pore diameter of the polypropylene hollow tubular body is 0.005-0.01 mm. micron, the microalgae used for inoculation is Nannochloropsis with a concentration of 10% algae liquid, and the cell diameter is 2-6 mm.

[0101] 1. Vaccination:

[0102] The polypropylene hollow tubular body 1 is put into the algae liquid pool whose concentration of the algae liquid is 10%, see Figure 5 , open the decompression pump 8 and the third valve 43, so that the pressure in the polypropylene hollow tubular body 1 is 0.1-0.6 MPa, and the culture solution in the algae liquid pool enters through the pores on the polypropylene hollow tubular body 1 The polypropylene hollow tubular body 1 is emptied by the decompression pump 8, and the algae cells in the algae liquid pool are blocked outside the polypropylene hol...

Embodiment 2

[0110] The hollow tubular body used in Example 2 is made of ceramic material through a sintering process, and the pore diameter is in the range of 0.01-0.5 microns. The microalgae used for inoculation is Nannochloropsis pseudochloropsis with an algae liquid concentration of 10%. Cells are 2-6 mm in diameter.

[0111] 1. Vaccination:

[0112] The ceramic hollow tubular body 1 is put into the algae liquid pool whose concentration of the algae liquid is 10%, see Figure 5 , open the decompression pump 8 and the third valve 43, so that the pressure in the ceramic hollow tubular body is 0.1-0.6MPa, and the culture solution in the algae liquid pool enters the ceramic hollow through the pores on the ceramic hollow tubular body 1. In the tubular body 1, it is evacuated by the decompression pump 8, and the algae cells in the algae liquid pool are blocked outside the ceramic hollow tubular body 1 and attached to the immobilized culture surface. The thickness of the algae cells after in...

experiment example

[0120] In order to objectively evaluate the cell detachment and microalgae cultivation of the examples 1-2, the specific experiments will be described in detail next.

[0121] 1. Test sample

[0122] The culture fluid falling into the collection tank 5 and the harvested microalgae during the immobilized cultivation process in the comparative example and the embodiment 1-2.

[0123] 2. Test and analysis method:

[0124] Measure the OD value at a wavelength of 750nm by a spectrophotometer, that is, the concentration of the algae liquid.

[0125] 3. Experimental results:

[0126] Referring to Table 1, it is the OD value of the algae liquid concentration of the culture liquid flowing through the immobilized culture surface during the immobilized cultivation process of the comparative example and Examples 1-2.

[0127] Table 1

[0128]

OD value

Comparative example

＞0.8

Example 1

＜0.1

Example 2

＜0.1

[0129] Referring to Ta...

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Abstract

The invention relates to the field of immobilized cultivation, and particularly relates to an immobilized cultivation device, a harvesting device and an immobilized cultivation method. The nutrient solution is supplied evenly, cell detachment is reduced and the cultivation yield is increased. The defects of the prior art that the nutrient solution is supplied directly to flush the cultivation surface and easily cause the cell detachment and the nutrient solution is unevenly supplied through the nutrient solution supply mode can be overcome. The immobilized cultivation device comprises a storage device and a cultivation device, wherein the storage device is used for providing microalgae nutrient solution; the cultivation device comprises at least one hollow tubular body, the outer surface of the hollow tubular body is a microalgae immobilized cultivation surface, the hollow tubular body is communicated with the storage device, pores are formed in the hollow tubular body, and nutrient solution flowing through the interior of the hollow tubular body is penetrated through the immobilized cultivation surface through the pores.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to an immobilized cultivation device, a harvesting device and an immobilized cultivation method. Background technique [0002] Immobilized culture technology is an emerging culture technology that can be applied to large-scale culture of various microorganisms. Immobilized culture refers to the process of immobilizing free cells or microorganisms on a certain carrier, making them highly dense and maintaining biological activity, and rapidly proliferating under suitable conditions. The concentration of microorganisms and the ability of microorganisms to resist unfavorable environments can be improved through immobilized cultivation, and solid-liquid separation is facilitated after cultivation is completed, which can shorten the harvesting time. [0003] At present, the fixation methods of immobilized culture mainly include adsorption method, embedding method, cross-linking meth...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N1/12C12R1/89
CPCC12M21/02C12M25/10C12M29/18C12M47/02C12N1/12
Inventor 张凯
Owner ENN SCI & TECH DEV
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