Breeding method of anthocyanin-rich black rice thermo-sensitive two-line sterile line

Abstract

The invention discloses a breeding method of an anthocyanin-rich black rice thermo-sensitive two-line sterile line and belongs to the technical field of special rice breeding. The method comprises the following steps: taking black rice with the brown rice anthocyanin color value which is more than 20 as a male parent, and taking a thermo-sensitive two-line sterile line as a female parent, and carrying out artificial hybridization; selecting single plants which has the brown rice anthocyanin color value more than 20, stable fertility, low critical temperature, long stable sterile period, thick and strong plant stalks, strong tillering, up-right leaves, small leaf angles and many spike grains in each generation from the generation F2; and finally, breeding to form the anthocyanin-rich black rice thermo-sensitive two-line sterile line. By virtue of the method, the foundation is laid for the assembling of hybrid black rice high-quality seeds which are rich in anthocyanin and have stable yield and high yield. The bred sterile line is used for breeding hybrid black rice varieties so that the bred hybrid black rice has good plant shape, high yield and stable yield; and the brown rice has bright color and luster, is dull black and is rich in anthocyanin, and has advantages of hybrids.

Description

technical field

[0001] The invention belongs to the technical field of special rice breeding, and in particular relates to a method for selecting a temperature-sensitive two-line male sterile line of black rice rich in anthocyanins. Background technique

[0002] With the continuous improvement of people's living standards in our country, people's requirements for rice nutrition and health care functions are constantly improving. Rice breeding has also shifted from focusing solely on yield to both yield and quality, and is increasingly emphasizing the directional breeding of functional components of special rice.

[0003] Black rice, also known as umami, has high nutritional and medicinal values. It has been recognized as a nourishing product since ancient times, and it is also a tonic enjoyed by the imperial palaces of all dynasties. Rice", "medicinal rice" and "immortal rice" have been cultivated in my country for more than 1,500 years. It is recorded in the pharmacy works...

Examples

Embodiment 1

[0020] 1) Using the black rice "Nantan84" with an anthocyanin color value of 20.60 as the male parent and the temperature-sensitive two-line male sterile line 272S that is helpful for the accumulation of hybrid anthocyanins as the female parent, carry out artificial hybridization, and plant the hybrid F 1 generation;

[0021] 2) Plant F 2 Generation, F 2 The generation population is more than 2,000 plants, and the planting site is selected in a place where the temperature of rice can be kept below 23.5°C from the stage of pistil formation to the meiosis stage of pollen mother cells (that is, the 4-6 stage of young panicle differentiation), and the fertility screening is carried out. For single CMS plants with strong stems, strong tillers, upright leaves, small leaf angles, and many spikes, the anthocyanin content of the selected individual brown rice plants is determined, and the ones with an anthocyanin color value of 20 or more are selected. Single plant;

[0022] 3) F 3...

Embodiment 2

[0027] 1) Using the black rice "Nantan84" with an anthocyanin color value of 20.60 as the male parent and the light-temperature-sensitive two-line male sterile line Guangzhan 63S as the female parent, artificially cross and plant the hybrid F 1 generation;

[0028] 2) Plant F 2 Generation, F 2 The generation population is more than 2,000 plants, and the planting site is selected in a place where the temperature of rice can be kept below 23.5°C from the stage of pistil formation to the meiosis stage of pollen mother cells (that is, the 4-6 stage of young panicle differentiation), and the fertility screening is carried out. For single CMS plants with strong stems, strong tillers, upright leaves, small leaf angles, and large number of spikes, the anthocyanin content of the selected individual plants of brown rice is determined, and the individual plants with high anthocyanin content are selected. However, the color price of anthocyanins selected from a single plant is lower tha...

Embodiment 3

[0034] 1) Using the black rice "Duck Blood Nuo" with an anthocyanin color value of 16.37 as the male parent and the temperature-sensitive two-line male sterile line 272S as the female parent, artificially cross and plant the hybrid F 1 generation;

[0035] 2) Plant F 2 Generation, F 2 The generation population is more than 2,000 plants, and the planting site is selected in a place where the temperature of rice can be kept below 23.5°C from the stage of pistil formation to the meiosis stage of pollen mother cells (that is, the 4-6 stage of young panicle differentiation), and the fertility screening is carried out. For single CMS plants with strong stems, strong tillers, upright leaves, small leaf angles, and large number of spikes, the anthocyanin content of the selected individual plants of brown rice is determined, and the individual plants with high anthocyanin content are selected. However, the color price of anthocyanins selected from a single plant is lower than 20;

[0...

technical field

[0001] The invention belongs to the technical field of special rice breeding, and in particular relates to a method for selecting a temperature-sensitive two-line male sterile line of black rice rich in anthocyanins. Background technique

[0002] With the continuous improvement of people's living standards in our country, people's requirements for rice nutrition and health care functions are constantly improving. Rice breeding has also shifted from focusing solely on yield to both yield and quality, and is increasingly emphasizing the directional breeding of functional components of special rice.

[0003] Black rice, also known as umami, has high nutritional and medicinal values. It has been recognized as a nourishing product since ancient times, and it is also a tonic enjoyed by the imperial palaces of all dynasties. Rice", "medicinal rice" and "immortal rice" have been cultivated in my country for more than 1,500 years. It is recorded in the pharmacy works...

Examples

Embodiment 1

[0020] 1) Using the black rice "Nantan84" with an anthocyanin color value of 20.60 as the male parent and the temperature-sensitive two-line male sterile line 272S that is helpful for the accumulation of hybrid anthocyanins as the female parent, carry out artificial hybridization, and plant the hybrid F 1 generation;

[0021] 2) Plant F 2 Generation, F 2 The generation population is more than 2,000 plants, and the planting site is selected in a place where the temperature of rice can be kept below 23.5°C from the stage of pistil formation to the meiosis stage of pollen mother cells (that is, the 4-6 stage of young panicle differentiation), and the fertility screening is carried out. For single CMS plants with strong stems, strong tillers, upright leaves, small leaf angles, and many spikes, the anthocyanin content of the selected individual brown rice plants is determined, and the ones with an anthocyanin color value of 20 or more are selected. Single plant;

[0022] 3) F 3...

Embodiment 2

[0027] 1) Using the black rice "Nantan84" with an anthocyanin color value of 20.60 as the male parent and the light-temperature-sensitive two-line male sterile line Guangzhan 63S as the female parent, artificially cross and plant the hybrid F 1 generation;

[0028] 2) Plant F 2 Generation, F 2 The generation population is more than 2,000 plants, and the planting site is selected in a place where the temperature of rice can be kept below 23.5°C from the stage of pistil formation to the meiosis stage of pollen mother cells (that is, the 4-6 stage of young panicle differentiation), and the fertility screening is carried out. For single CMS plants with strong stems, strong tillers, upright leaves, small leaf angles, and large number of spikes, the anthocyanin content of the selected individual plants of brown rice is determined, and the individual plants with high anthocyanin content are selected. However, the color price of anthocyanins selected from a single plant is lower tha...

Embodiment 3

[0034] 1) Using the black rice "Duck Blood Nuo" with an anthocyanin color value of 16.37 as the male parent and the temperature-sensitive two-line male sterile line 272S as the female parent, artificially cross and plant the hybrid F 1 generation;

[0035] 2) Plant F 2 Generation, F 2 The generation population is more than 2,000 plants, and the planting site is selected in a place where the temperature of rice can be kept below 23.5°C from the stage of pistil formation to the meiosis stage of pollen mother cells (that is, the 4-6 stage of young panicle differentiation), and the fertility screening is carried out. For single CMS plants with strong stems, strong tillers, upright leaves, small leaf angles, and large number of spikes, the anthocyanin content of the selected individual plants of brown rice is determined, and the individual plants with high anthocyanin content are selected. However, the color price of anthocyanins selected from a single plant is lower than 20;

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