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Breeding method of three-line hybrid rice of aromatic purple rice

A technology of three-line hybrid rice and purple rice, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of low yield and inability to meet market demand, and achieve high yield, strong synergy and adaptability strong effect

Inactive Publication Date: 2015-09-23
文山壮族苗族自治州农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of rich genetic basis and expanded genetic differences of hybrid rice, the yield of these conventional rice is always lower than that of hybrid rice by more than 30%. As a result, although the quality of conventional rice of fragrant purple rice is very good, the yield is low and cannot meet the market demand.

Method used

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  • Breeding method of three-line hybrid rice of aromatic purple rice
  • Breeding method of three-line hybrid rice of aromatic purple rice
  • Breeding method of three-line hybrid rice of aromatic purple rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] (1) Screening maintainer line I and restorer line VI with purple rice gene (P) and aroma gene (a):

[0062] Excellent cytoplasmic male sterile lines with the same relationship of restoration and maintenance: Yebai type CMS line Longtepu A, Gang type CMS line Gang 46A, D type CMS line Yixiang 1A or Yinshui type CMS line Ⅱ-32A As the female parent, Wenzi 128, Shiping Zimi, Banna Zimi, and Wenzi 386 were used as the male parent. In July 2004, they were crossed in the network room of Wenshan Academy of Agricultural Sciences, and the first-generation hybrid seeds were obtained. ;

[0063] In August 2005, at the experimental base of Wenshan Academy of Agricultural Sciences, the planted F 1 The seed setting rate was determined, the hybrid F of Longtepu A, Gang 46A, Yixiang 1A and Ⅱ-32A, Wenzi 128 and Banna Zimi 1 The seed-setting rate of the generation is 0%, and Wenzi 128 has a strong fragrance and a good plant type. When crossed with the excellent maintainer line II, it is...

Embodiment 2

[0085] Example 2 Breeding of fragrant purple rice hybrid rice Wenzixiang 206

[0086] Wenzixiang 426A was used as the female parent, which is the three-line CMS line V of fragrant purple rice, and Wenhui 206 was used as the male parent, that is, the fragrant non-purple rice rice restorer line with the same allelic fragrance gene as Wenzixiang 426A. Combination breeding of aromatic purple rice hybrid rice variety Ⅳ. Specifically:

[0087] In March 2013, Wenzixiang 426A, a three-line CMS line of fragrant purple rice, was used as female parent and Wenhui 206, a restorer line of fragrant non-purple rice, was used as male parent in the Sanya base of Wenshan Academy of Agricultural Sciences to form a hybrid combination. get F 1 generation seeds.

[0088] In April 2013, the hybrid combination F was planted in Yunnan Wenshan, Honghe, Xishuangbanna, Sanya, Guiyang, Mianyang and other places 1 Substitute seeds, conduct multi-point identification test, and identify F 1 The aroma of ...

Embodiment 3

[0090] Example 3 Breeding of fragrant purple rice hybrid rice Wenxiang 648

[0091] Wenzihui 648 was used as the male parent, that is, the fragrant purple rice restorer line VIII, and Wenxiang 28A was used as the female parent, that is, the fragrant non-purple rice rice male sterile line with the same allelic fragrance gene as Wenzihui 648. Yuxiang type purple rice hybrid rice variety Ⅳ. Specifically:

[0092] In March 2013, Wenzihui 648, a restorer line of fragrant purple rice, was used as the male parent, and Wenxiang 28A, a three-line CMS line of fragrant non-purple rice, was used as the female parent in the Sanya Base of Wenshan Academy of Agricultural Sciences in March 2013. f 1 generation seeds.

[0093] In April 2013, the hybrid combination F was planted in Wenshan, Honghe, Xishuangbanna and other cities in Yunnan, Sanya in Hainan, Guiyang in Guizhou, and Mianyang in Sichuan. 1 Substitute seeds, conduct multi-point identification test, and identify F 1 The aroma of...

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Abstract

The invention discloses a breeding method of three-line hybrid rice of aromatic purple rice. The method comprises the following steps: (1) screening a maintaining line I and a restoring line VI of the aromatic purple rice, with homoallelic purple rice genes and aromatic genes, to be used as parental donor materials for breeding purple rice genes (P) and aromatic genes (a) of the maintaining line and the restoring line of the aromatic purple rice; (2) respectively hybridizing the maintaining line I and the restoring line VI with a maintaining line with high combining ability and a restoring line with high combining ability, and bringing in the purple rice genes (P) and the aromatic genes (a) so as to obtain a maintaining line III and a restoring line VIII of aromatic (aa) purple rice (PP); hybridizing and backcrossing the maintaining line III of aromatic (aa) purple rice (PP) with a three-line sterile line so as to obtain a three-line sterile line V of aromatic (aa) purple rice (PP); (3) mating the sterile line V with the restoring line VIII so as to obtain the hybrid rice IX of aromatic (aa) purple rice PP or Pp). The obtained hybrid rice of the aromatic purple rice is strong in aroma, has a purple seed coat, is high in yield, and is wide in application range.

Description

technical field [0001] The invention belongs to the field of hybrid seed production of rice, and relates to a method for breeding fragrant purple rice three-line hybrid rice. Background technique [0002] Purple rice, also known as black rice, is a kind of rice that is named for its black and purple appearance due to the accumulation of anthocyanins or anthocyanins in the seed coat and pericarp of rice, which makes brown rice appear purple red, purple black, jet black and other colors. Purple rice is used both as medicine and food, and has many health benefits, so it is also called medicinal rice, blood-enriching rice, moon rice, longevity rice, tribute rice and pearl rice. There are two types of rice quality: waxy and sticky, but most of them are waxy. [0003] Traditional Chinese medicine believes that purple glutinous rice can nourish the kidneys, improve the functions of the heart, liver, spleen, and stomach, strengthen the foundation and strengthen the body, nourish qi...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 李存龙罗龙韦永贵陶永宏董卓娅张晓磊
Owner 文山壮族苗族自治州农业科学院
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