A screening method for pathogenic insecticidal bacteria against scutellaria in seawater fish

Abstract

The invention provides a screening method for pathogenic insecticidal bacteria of seawater fish shield ciliates, which is characterized in that it comprises the following steps: (1), separating and purifying marine bacteria; (2), separating and screening seawater fish shield ciliates Pathogen; (3), the isolated marine bacteria and shield ciliates are co-cultured in a 96-well plate; (4), the survival rate of the shield ciliates in the co-cultivation system is monitored to determine the insecticidal bacteria. Positive effect of the present invention: on the basis of previous research, by the present invention, can carry out the screening of marine bacterium in large quantities, rapidly, and obtain the bacterial strain that action effect is remarkable to target shield ciliate that clear killing effect is arranged, thereby improved The possibility of using microbial methods to control shield ciliate disease has the advantages of short time consumption, high efficiency, no pollution in the whole experimental process, and can meet the requirements of green environmental protection.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a screening method for pathogenic insecticidal bacteria of seawater fish scutellaria. Background technique [0002] Seawater fish factory farming is a very important industry in my country's aquaculture. However, with the continuous improvement of aquaculture production and the continuous expansion of aquaculture scale, various diseases have become more frequent, among which shield ciliate disease is the most harmful kind of. Scutellum ciliates are facultative parasites, generally free-living, and feed on suspended tiny particulate matter (bacteria, microalgae, protozoa, etc.); however, under certain circumstances, these ciliates can be parasitic, Phagocytosis of fish cells and tissue residues and growth and reproduction in their tissues; reports show that shield ciliates can occur in factory farming workshops across my country from 12 to 26 °C, almost all year round; shi...

AI Technical Summary

Problems solved by technology

[0003] In order to prevent and control the harm of shield ciliate disease to my country's seawater fish farming industry, many domestic units have screened the control drugs for shield ciliate, but the treatment of the disease in aquatic products is still concentrated on formalin and copper sulfate. 1. The use of zinc sulfate. These drugs basically act on protein denaturation. Although they can kill shield ciliates in water, it is difficult to completely kill shield ciliates in fish, and these drugs have no ability to distinguish between fish and human cells. , can also play a role and cause serious consequences. In addition, Chinese patent documents CN1762460A and CN103349747A have announced two Chinese herbal preparations for the prevention and treatment of flounder scutellum ciliates, but the application and stability of these preparations in actual production still need more tests

[0004] In fact, in nature, there are many microorganisms that have pathogenic or killing effects on shield ciliates. Using the pathogenicity of microorganisms to prevent and control shield ciliates will be an effective biological control method. In the field of pest control, its research began in the 19th century, and gradually entered the development and practical stage in the first half of the 20th century. Microbial pesticides are the product of the combination of biological science and engineering technology. With the rapid development of modern bioengineering, microbial pesticides will There is a great development prospect, but according to the current data, there is no report on the method of screening insecticidal bacteria for the pathogen of shield ciliates in seawater fish