Molecular marker tightly linked to fiber fineness of ramie, obtaining method and application thereof

Abstract

The invention discloses a molecular marker tightly linked to fiber fineness of ramie and also discloses a method for obtaining the molecular marker tightly linked to fiber fineness of ramie. The method comprises the following steps: 1, a ramie germplasm SSR marker genetype is obtained; 2, the SSR marker genetype is analyzed and a K matrix graph is calculated; 3, a Q-value matrix is generated by the utilization of the SSR marker genetype; 4, by using the K matrix graph and the Q-value matrix as covariance, molecular marker data and fiber fineness quantitative character data are tested, and P and R2 (phenotypic variation interpretation ratio) data are output; 5, by using a PCA matrix and the K matrix graph as covariance, the molecular marker data and fiber fineness quantitative character data are tested, and P and R2 data are output; and 6, a SSR locus with P being less than 0.05 and R2 being less than 0.82 and greater than 0.45 is selected so as to obtain the molecular marker which controls fiber fineness of ramie and is tightly linked to fiber fineness of ramie. The invention also discloses an application of the molecular marker in ramie variety breeding and fiber fineness enhancement.

Description

technical field [0001] The invention relates to a molecular marker closely linked with the fineness of ramie fiber, its obtaining method and its use. Background technique [0002] Association analysis is a research method based on natural variation populations and using the law of linkage disequilibrium to study the relationship between genetic variation and target traits (Mackay et al., 2007). Compared with traditional QTL, association analysis does not need to construct a mapping population, has a large breadth, high precision, and can detect multiple alleles at the same locus (Meuwissen et al., 2000; Khush et al., 2001). In 2001, Thornsberry et al. (2001) successfully applied association analysis to plants for the first time, and found that the dwarf8 gene was not only related to gibberellin metabolism, but also could affect the plant height of maize. This result shows that LD-based association analysis can be used to verify gene functions, as well as gene mining, and it...

AI Technical Summary

Problems solved by technology

However, at present, there are still few studies on genes related to ramie fiber fineness development. She Wei et al. (2007) constructed two high-quality ramie stem bark cDNA libraries, obtained 8 fiber development-related gene sequences, and obtained 275 Valid ESTs

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