A kind of method of preventing and treating verticillium wilt

A kind of technology of cotton verticillium wilt and biocontrol bacteria

Active Publication Date: 2017-08-01
新疆康润洁环保科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are two main ways to solve the "white pollution" caused by plastics. One is to recycle the used plastics, but this is restricted by the use of plastics, categories, and economics. Generally speaking, recyclable plastics Limited, most of these plastics are made of petroleum, which cannot be degraded in the natural environment after use, and can also resist bacterial erosion, so the recovery rate is low, so it cannot completely and effectively solve the "white pollution" "Secondly, vigorously promote the use of degradable plastics, which is one of the effective ways to solve the problem of "white pollution"
However, most of the degradable plastic base materials are polyester polymer materials. Since the molecular arrangement of polyester is a linear arrangement during the synthesis process, when it is made into finished agricultural mulch film, the longitudinal tear strength is too low to meet the normal requirements. Therefore, the molecular chain arrangement of the molecule is changed during the synthesis, its branching degree is improved, and the "knot" effect between the molecules is effectively improved, thereby improving the physical and mechanical properties of the polyester polymer material. Meet the requirements of film laying required for normal crop growth

Method used

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  • A kind of method of preventing and treating verticillium wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Preparation of biocontrol bacteria powder

[0045] Biocontrol bacteria-Bacillus subtilis were isolated from cotton rhizosphere soil. The Bacillus subtilis stored on the slant was activated on a PDA plate, and the bacteria were collected after picking the colony and shaking it in the PD medium. The collected Bacillus subtilis was placed in 1000 mL of liquid NA medium, and the Bacillus subtilis fermentation broth was obtained after shaking and culturing for 40 hours at a temperature of 25-28° C. and a rotation speed of 300 r / min. The Bacillus subtilis fermentation broth was centrifuged at 4800 r / min for 15 min at room temperature, and the supernatant was taken, mixed with 30 g of macroporous resin Amberlite XAD16, shaken at 25 °C and 200 r / min for 3 hours, and then filled into the column. Wash the column with distilled water first, then eluate with acetone, and collect the acetone eluent. The acetone eluate was concentrated by rotary evaporation at 40°C, and finally...

Embodiment 2

[0054] The preparation of step (1) biocontrol bacteria powder and the preparation of step (2) polyester biodegradable raw material are the same as in Example 1.

[0055] Step (3) Preparation of Biodegradable Mulch

[0056] The biocontrol bacteria powder containing 3% PVP is added in the auxiliary pipeline 7, wherein the biocontrol bacteria powder is 3.0Kg, and the others are the same as the step (3) in Example 1;

[0057] A kind of polyester biodegradable mulch film prepared above for preventing and treating cotton verticillium wilt was placed in the middle of the two-layer filter screen and buried 25 cm under the soil, and the degradation residue in the middle of the two layers was taken out after complete biodegradation. . The residue was evenly sprinkled on the cotton field. The results showed that compared with the cotton field control without biodegradable plastic film, the incidence rate of cotton verticillium wilt was 7.8%, and the disease prevention efficiency was 80%...

Embodiment 3

[0059] The preparation of step (1) biocontrol bacteria powder and the preparation of step (2) polyester biodegradable raw material are the same as in Example 1.

[0060] Step (3) Preparation of Biodegradable Mulch

[0061] The biocontrol bacteria powder and seaweed fertilizer containing 3% PVP are added in the auxiliary pipeline 7, wherein the biocontrol bacteria powder and the seaweed fertilizer are each 3.0Kg, and the others are as in step (3) of Example 1;

[0062] A kind of polyester biodegradable mulch film prepared above for preventing and treating cotton verticillium wilt was placed in the middle of the two-layer filter screen and buried 25 cm under the soil, and the degradation residue in the middle of the two layers was taken out after complete biodegradation. . The residue was evenly sprinkled on the cotton field. The results showed that compared with the cotton field control without biodegradable plastic film, the incidence rate of cotton Verticillium wilt was 1.8%...

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Abstract

The invention relates to a method for preventing and treating greensickness through cooperation of an agricultural biodegradable mulching plastic film and biocontrol bacteria. According to the method in which cooperation between the biodegradable mulching plastic film and the biocontrol bacteria is used, the greensickness can be prevented and treated more effectively, thereby realizing higher efficiency and longer duration.

Description

technical field [0001] The invention relates to a method for preventing and treating verticillium wilt of cotton, in particular to a method for preventing and treating verticillium wilt by combining agricultural biodegradable plastic film and biocontrol bacteria. Background technique [0002] Cotton verticillium wilt is a disease caused by pathogens in the soil invading crops from the roots or stems of crops when the conditions are suitable. The pathogens are mainly transmitted through soil organic fertilizers, irrigation water or natural running water. Blocks can reduce crop yields by 30%-50%. [0003] Biological control is the natural control of harmful organisms by natural beneficial organisms in the agricultural ecosystem. The biological control of plant diseases has attracted more and more attention due to its safety, effectiveness, low toxicity and less residue. The use of biological control has undoubtedly become a radical cure. In addition, biological control can ov...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G13/02A01N63/00A01P3/00
CPCA01G9/1438A01G13/0275A01N63/00Y02A40/28
Inventor 孔琳仪孔立明任建林陈志伟
Owner 新疆康润洁环保科技股份有限公司
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