A breeding method for inducing micro-adventitious buds from blueberry Legacy leaves
A technology of micro-adventitious leaves, applied in the field of breeding of blueberry Legacy leaves to induce micro-adventitious buds
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[0039] (1) Cultivation of sterile test-tube seedlings: select the 10cm long top tip of the young shoots of the year, remove the leaves, cut into 2 bud stem sections, inoculate into the stem section side bud starting medium after disinfection, place in the tissue culture room, The light intensity during the day is 2500Lux, and the light time is 13h·d -1 , the day temperature is 25°C, the night temperature is 18°C, after 20 days the lateral buds germinate, and sterile seedlings are obtained;
[0040] (2) Leaf callus culture: cut off the leaf edge of the aseptic seedling leaves cultivated in step (1), scratch 2 blades horizontally along the veins, inoculate in the medium for culturing leaf callus, and place in an incubator in the dark. Cultured at 25°C, after 15 days, light yellow callus of leaves was obtained;
[0041] (3) Leaf microadventitious bud differentiation: the light yellow callus of the leaves cultivated in step (2) was cut into 0.4cm 3 block, inoculated into the lea...
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