A breeding method for inducing micro-adventitious buds from blueberry Legacy leaves

A technology of micro-adventitious leaves, applied in the field of breeding of blueberry Legacy leaves to induce micro-adventitious buds

Active Publication Date: 2017-07-14
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few domestic and foreign literatures on tissue culture and rapid propagation of blueberry legacy, and the breeding method of using blueberry legacy leaves to induce micro-adventitious buds and micro-adventitious buds to form seedlings has not been reported yet.

Method used

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  • A breeding method for inducing micro-adventitious buds from blueberry Legacy leaves
  • A breeding method for inducing micro-adventitious buds from blueberry Legacy leaves
  • A breeding method for inducing micro-adventitious buds from blueberry Legacy leaves

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Embodiment 1

[0039] (1) Cultivation of sterile test-tube seedlings: select the 10cm long top tip of the young shoots of the year, remove the leaves, cut into 2 bud stem sections, inoculate into the stem section side bud starting medium after disinfection, place in the tissue culture room, The light intensity during the day is 2500Lux, and the light time is 13h·d -1 , the day temperature is 25°C, the night temperature is 18°C, after 20 days the lateral buds germinate, and sterile seedlings are obtained;

[0040] (2) Leaf callus culture: cut off the leaf edge of the aseptic seedling leaves cultivated in step (1), scratch 2 blades horizontally along the veins, inoculate in the medium for culturing leaf callus, and place in an incubator in the dark. Cultured at 25°C, after 15 days, light yellow callus of leaves was obtained;

[0041] (3) Leaf microadventitious bud differentiation: the light yellow callus of the leaves cultivated in step (2) was cut into 0.4cm 3 block, inoculated into the lea...

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Abstract

The invention discloses a breeding method for inducing micro-adventitious buds from leaves of blueberry Legacy. The steps include: (1) cultivation of sterile test-tube plantlets; (2) cultivation of leaf callus; (3) differentiation of micro-adventitious buds from leaves (4) micro-adventitious buds become seedlings (5) test-tube seedlings take root; (6) test-tube seedlings are transplanted; The bud callus is transferred to the seedling medium to obtain a large number of seedling plants, which solves the technical problem of producing micro-adventitious buds from blueberry Legacy leaves and difficult seedling formation from the micro-adventitious buds. Using the technical method of the invention can provide a large amount of high-quality tissue culture seedlings in a relatively short period of time, reaching the standard of rapid industrial seedling cultivation, and meeting the domestic demand for high-quality blueberry Legacy seedlings.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a breeding method for inducing micro-adventitious buds from leaves of blueberry Legacy. Background technique [0002] Blueberry (Blueberry) belongs to Ericaceae (Ericaceae) Vaccinium spp., a perennial green leaf or evergreen shrub. Human body immunity and other functions. It is precisely because of the nutritional and medicinal health functions of blueberries that the International Food and Agriculture Organization has listed them as one of the five health foods for human beings. [0003] In the 1980s, Jilin Agricultural University took the lead in carrying out blueberry research in my country. On the basis of a full investigation of the wild blueberry resources in our country, it began to introduce blueberries from abroad in 1983. Up to now, more than 150 excellent varieties have been introduced, including blueberry Lager. West (Legacy) belongs to northern highbush blueberry (Vac c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王开芳刘翠兰燕丽萍孙蕾夏阳李丽
Owner SHANDONG FOREST SCI RES INST
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