A method for establishing a mango stem segment sterile system

A stem section and mango technology, which is applied in the field of establishment of mango stem section aseptic system, can solve the problems of mango difficulty and short survival period of seeds, etc.

Active Publication Date: 2017-09-26
BAISE UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mango is a woody plant with highly heterozygous genes, and the survival period of seeds is not long. It is difficult to improve mango with traditional breeding methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The following examples further illustrate the present invention.

[0015] A method for the establishment of a mango stem segment sterile line system, the method may further comprise the steps:

[0016] 1) Mango twig stems are used as pretreatment of explants: Mango twig stems are freshly drawn twigs from mango trees, leaves are cut off, 0.5cm of petiole is left, and the branches are cut into 1.5cm long strips. The stem section of the axillary bud, as explant, in the present embodiment, washes the stem section with running water for 10 minutes, then soaks with 0.01% potassium permanganate solution for 15 minutes, then rinses with running water for 30 minutes, and rinses for subsequent use;

[0017] 2) Sterilization: Place the stem section treated in step 1) on a sterilized ultra-clean workbench, sterilize the stem section with 75% alcohol for 20 seconds, then rinse it with sterile water for 3 times, and then wash it with 0.1 %HgCl 2 and 3 drops of Tween-80 for 13 minut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for establishing a sterile system of mango stem segments, and relates to the field of plant tissue culture. The method comprises the following steps: 1) cutting mango twigs and stems as explant pretreatment; 2) sterilization; 3) inoculation; 4) incandescent light culture; 5) secondary sterilization; 6) obtaining regenerated plants . The invention breaks the traditional way of obtaining mango regenerated plants by somatic embryogenesis, can inherit the excellent characters of the mother plant, obtain a large number of mango tissue culture seedlings, realize the cultivation of mango stem segments and induce sterile buds, and achieve the effect of rapid reproduction.

Description

【Technical field】 [0001] The invention belongs to the field of plant tissue culture, and relates to a method for establishing a mango stem segment sterile line system. 【Background technique】 [0002] Mango (mangifera indica) belongs to the Anacardiaceae (anacardiaceae) mango genus (meagifera), is one of the tropical high-quality fruits, and is the second largest tropical fruit in the world. Mango has tender meat, strong flavor and rich nutrition. It has twelve kinds of essential amino acids, vitamins A and C, and carotene. Eating mango has the effects of anti-cancer, beautifying skin, preventing high blood pressure, arteriosclerosis, preventing constipation, relieving cough, and clearing the stomach. It is a famous tropical and subtropical fruit that consumers love very much, and is known as the "king of tropical fruits". China is the second largest mango producer after India. The mango cultivation areas are mainly distributed in Hainan, Guangxi, Guangdong, Yunnan and the d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 农艳丰岑湘涛苏仕林
Owner BAISE UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap