A method for establishing a mango stem segment sterile system
A stem section and mango technology, which is applied in the field of establishment of mango stem section aseptic system, can solve the problems of mango difficulty and short survival period of seeds, etc.
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[0014] The following examples further illustrate the present invention.
[0015] A method for the establishment of a mango stem segment sterile line system, the method may further comprise the steps:
[0016] 1) Mango twig stems are used as pretreatment of explants: Mango twig stems are freshly drawn twigs from mango trees, leaves are cut off, 0.5cm of petiole is left, and the branches are cut into 1.5cm long strips. The stem section of the axillary bud, as explant, in the present embodiment, washes the stem section with running water for 10 minutes, then soaks with 0.01% potassium permanganate solution for 15 minutes, then rinses with running water for 30 minutes, and rinses for subsequent use;
[0017] 2) Sterilization: Place the stem section treated in step 1) on a sterilized ultra-clean workbench, sterilize the stem section with 75% alcohol for 20 seconds, then rinse it with sterile water for 3 times, and then wash it with 0.1 %HgCl 2 and 3 drops of Tween-80 for 13 minut...
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