A medium combination for tissue culture and rapid propagation of Lily Medog, tissue culture and rapid propagation method and application

A technology of lily tissue culture and culture medium, applied in the direction of culture medium, application, gardening methods, etc., to achieve rapid reproduction, simplify steps, and solve the effects of sporadic distribution in the wild

Active Publication Date: 2021-11-05
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no tissue culture and rapid propagation method for Lilium Medog at present, therefore, there is an urgent need for a method for rapid propagation of tissue culture for Lily Medog

Method used

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  • A medium combination for tissue culture and rapid propagation of Lily Medog, tissue culture and rapid propagation method and application
  • A medium combination for tissue culture and rapid propagation of Lily Medog, tissue culture and rapid propagation method and application
  • A medium combination for tissue culture and rapid propagation of Lily Medog, tissue culture and rapid propagation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Inducing and differentiation medium screening

[0061] Taking the scales on the ink extolizes as an explant, first wash the mud on the scales with tap water, and then soak 8 minutes with 10% of the gold spray disinfectant. After the filtration is flushed 3 times, get the super net After 75% alcohol disinfection 10s, the bacteria was rinsed 3 times, then disinfected with 0.1% liter withdrozencalline 7min, sterile water was cleaned 3 to 5 times, resulting in disinfection of the implant, spare.

[0062] The sterilized explants were inoculated in the following five induced and differentiated media, and each bottle was placed in one scales, concave up. The above five induced and differentiated mediums are: 1) modified MS medium (micro element plus 1 times) + 0.1 mg / l 6-ba + 0.1 mg / l naa; 2) improved MS medium (trace element) Plus 1 times) + 0.2 mg / l 6-ba + 0.2 mg / lnaa; 3) Improved MS medium (1 times of micro element plus 1 times) + 0.5mg / l 6-ba + 0.5mg / l na...

Embodiment 2

[0067] Example 2 Proliferation and completion medium screening

[0068] The medium fractionation of 5 gradients were provided with a substantially medium of proliferation and complex medium hormone screening with modified MS (same as in Example 1). 0.5 mg / L, 1.0 mg / L, 1.5 mg / l and 2.0mg / L, growth perisulin NAA set 2 gradients, 0.5mg / L and 1.0mg / L, respectively, using uniform design method, screening proper Proliferation and completion medium. The above value added and the subsequent medium also contain sucrose 45 g / L, agar 5g / L, pH 6.0. The conditions of proliferation and subsequent culture are: the light intensity is 1800 lux, the temperature is 25 ° C, the light period is 10 L: 24d, and the culture period is 50d. The statistical results are shown in Table 2.

[0069] Table 2 Proliferation and completion medium screening

[0070]

[0071] Remarks: "-" indicates an invincible organization; "+" indicates that the callus is 0.1cm or less; "++" indicates that the ca...

Embodiment 3

[0073] Example 3 Screening of Grounding and Rooting Medium

[0074] The medium fractionation of the splituminotin 6-ba was provided with a basic medium of the split seed culture medium hormone and the cell fractionation 6-Ba, which is 0.5mg / L in the basic medium and the cell fractionation 6-ba. And 1.0 mg / L, the growth of the growth perisulin NaA is set to 0.5 mg / L and 1.0 mg / L, respectively, screening suitable mass seedlings and rooted medium. The above varnisher and the rooting medium also contain sucrose 45 g / l, agar 5g / L, pH 6.0. The conditions for the cultivation of the mass seedlings and roots are: the light intensity is 1500-2000 Lux, the temperature is 25 ° C, the light period is 5 L: 19d, the culture period is 50d. Statistical 50D induced non-bound condition. The statistical results are shown in Table 3.

[0075] Table 3 Screening of Grounding and Rooting Culture

[0076]

[0077] Remarks: "-" indicates an invincible organization; "+" indicates that the call...

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Abstract

The invention provides a medium combination for tissue culture and rapid propagation of Lily Medog, a tissue culture and rapid propagation method and application thereof, and belongs to the technical field of plant tissue rapid propagation. By optimizing the components of the medium, the present invention combines the induction and differentiation medium into one, the proliferation and subculture medium into one, the strong seedling and rooting medium into one, and simplifies the Lilium medoguo The steps of tissue culture have realized the rapid reproduction of Lilium Medog, solved the problems of sporadic distribution of Lily Medog in the wild, few populations, and endangered populations, and provided comprehensive protection, development and sustainable utilization of Lily Medog, as well as preservation of germplasm resources. It laid the foundation and filled the gap in the research and development of Medog Lily in biotechnology.

Description

Technical field [0001] The present invention belongs to the technical field of plant tissue, and is specifically involved in medium combination of cultural solutions for the cultivation of the ink lilies. Background technique [0002] Lilium MedoGense is a new species published by Mr. Liang Songzhen in 1985. The model specimen is a specimen from Mr. Chen Weilie from Tibet, Tibet County in 1980. It is currently in the end of the hills, which is a typical homonymous wild plant, expanding reproduction and protection significance. There is currently no faster than the historical method for the group culture of the ink lily, so there is an urgent need for a group culture for the turtain lily. Inventive content [0003] In order to solve the above problems, the present invention provides a medium combination for cultivating medium for the cultivation of the ink lilies, and the faster method and application of the group culture. The combination of medium which is used in the inventory ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G24/15A01G24/28A01G24/23
CPCA01H4/008A01G24/15A01G24/23A01G24/28A01H4/002
Inventor 罗桂芬孙卫邦申建勇
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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