A medium combination for tissue culture and rapid propagation of Lily Medog, tissue culture and rapid propagation method and application
A technology of lily tissue culture and culture medium, applied in the direction of culture medium, application, gardening methods, etc., to achieve rapid reproduction, simplify steps, and solve the effects of sporadic distribution in the wild
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Embodiment 1
[0060] Example 1 Inducing and differentiation medium screening
[0061] Taking the scales on the ink extolizes as an explant, first wash the mud on the scales with tap water, and then soak 8 minutes with 10% of the gold spray disinfectant. After the filtration is flushed 3 times, get the super net After 75% alcohol disinfection 10s, the bacteria was rinsed 3 times, then disinfected with 0.1% liter withdrozencalline 7min, sterile water was cleaned 3 to 5 times, resulting in disinfection of the implant, spare.
[0062] The sterilized explants were inoculated in the following five induced and differentiated media, and each bottle was placed in one scales, concave up. The above five induced and differentiated mediums are: 1) modified MS medium (micro element plus 1 times) + 0.1 mg / l 6-ba + 0.1 mg / l naa; 2) improved MS medium (trace element) Plus 1 times) + 0.2 mg / l 6-ba + 0.2 mg / lnaa; 3) Improved MS medium (1 times of micro element plus 1 times) + 0.5mg / l 6-ba + 0.5mg / l na...
Embodiment 2
[0067] Example 2 Proliferation and completion medium screening
[0068] The medium fractionation of 5 gradients were provided with a substantially medium of proliferation and complex medium hormone screening with modified MS (same as in Example 1). 0.5 mg / L, 1.0 mg / L, 1.5 mg / l and 2.0mg / L, growth perisulin NAA set 2 gradients, 0.5mg / L and 1.0mg / L, respectively, using uniform design method, screening proper Proliferation and completion medium. The above value added and the subsequent medium also contain sucrose 45 g / L, agar 5g / L, pH 6.0. The conditions of proliferation and subsequent culture are: the light intensity is 1800 lux, the temperature is 25 ° C, the light period is 10 L: 24d, and the culture period is 50d. The statistical results are shown in Table 2.
[0069] Table 2 Proliferation and completion medium screening
[0070]
[0071] Remarks: "-" indicates an invincible organization; "+" indicates that the callus is 0.1cm or less; "++" indicates that the ca...
Embodiment 3
[0073] Example 3 Screening of Grounding and Rooting Medium
[0074] The medium fractionation of the splituminotin 6-ba was provided with a basic medium of the split seed culture medium hormone and the cell fractionation 6-Ba, which is 0.5mg / L in the basic medium and the cell fractionation 6-ba. And 1.0 mg / L, the growth of the growth perisulin NaA is set to 0.5 mg / L and 1.0 mg / L, respectively, screening suitable mass seedlings and rooted medium. The above varnisher and the rooting medium also contain sucrose 45 g / l, agar 5g / L, pH 6.0. The conditions for the cultivation of the mass seedlings and roots are: the light intensity is 1500-2000 Lux, the temperature is 25 ° C, the light period is 5 L: 19d, the culture period is 50d. Statistical 50D induced non-bound condition. The statistical results are shown in Table 3.
[0075] Table 3 Screening of Grounding and Rooting Culture
[0076]
[0077] Remarks: "-" indicates an invincible organization; "+" indicates that the call...
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