Culture medium combination for tissue culture and rapid propagation of Lilium medogense, tissue culture and rapid propagation method and application

A technology of tissue culture and medium of lily, applied in the directions of medium, application, planting substrate, etc., to achieve the effects of rapid reproduction, solving sporadic distribution in the field, and simplifying steps

Active Publication Date: 2021-08-31
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no tissue culture and rapid propagation method for Lilium Medog at present, therefore, there is an urgent need for a method for rapid propagation of tissue culture for Lily Medog

Method used

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  • Culture medium combination for tissue culture and rapid propagation of Lilium medogense, tissue culture and rapid propagation method and application
  • Culture medium combination for tissue culture and rapid propagation of Lilium medogense, tissue culture and rapid propagation method and application
  • Culture medium combination for tissue culture and rapid propagation of Lilium medogense, tissue culture and rapid propagation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 induced differentiation medium and screened embodiment

[0061] After taking on the scales of lily bulbs Motuo explants, washed first with tap water on the soil flakes, with 10% of the gold Wan spray disinfectant 8min, through the filtered water washed three times to get clean bench after disinfection with 75% alcohol 10s, sterile water 3 times, and then sterilized with 0.1% mercuric chloride for 7min, washed in sterile water 3-5 times to obtain explants sterilized aside.

[0062] The explants were inoculated in the sterilized following five kinds of induction and differentiation medium, the medium bottle a flat scales, concave upwardly. The above-mentioned five kinds of induction and differentiation medium were as follows: 1) modified MS medium (1x trace element) + 0.1mg / l 6-BA + 0.1mg / L NAA; 2) modified MS medium (Trace Elements plus 1x) + 0.2mg / l 6-BA + 0.2mg / LNAA; 3) modified MS medium (1x trace element) + 0.5mg / l 6-BA + 0.5mg / L NAA; 4) improvement...

Embodiment 2

[0067] Example 2 Screening proliferation embodiment subculture medium

[0068] (Same as Example 1 MS medium modified embodiment of the same) to modified MS medium for proliferation and minimal medium culture medium following screening hormone, cytokinin 6-BA 5 disposed gradients were 0.1mg / L , 0.5mg / L, 1.0mg / L, 1.5mg / L and 2.0mg / L, and NAA provided two gradients were 0.5mg / L and 1.0mg / L, for uniform design, selection of optimal proliferation and subculture medium. Each subculture medium added further with sucrose 45g / L, agar 5g / L, pH6.0. Following culture proliferation and conditions as follows: light intensity 1800LUX, a temperature of 25 ℃, photoperiod 10L: 24D, the culture period 50d. Statistical results are shown in Table 2.

[0069] Table 2 Screening of proliferation and subculture medium

[0070]

[0071] NOTE: "-" indicates no callus; "+" indicates callus 0.1cm or less; "++" indicates callus 0.1cm above.

[0072] From the results of Table 2, when the pro...

Embodiment 3

[0073] Screening seedling and rooting medium Example 3

[0074] (Modified MS medium identical with Example 1 of the embodiment) to be substantially modified MS medium medium medium seedling and rooting hormone screening, cytokinin 6-BA 2 gradient provided, respectively 0.5mg / L and 1.0mg / L, and NAA provided two gradients were 0.5mg / L and 1.0mg / L, and seedling screening a suitable rooting medium. Each seedling and rooting medium further contains sucrose 45g / L, agar 5g / L, pH6.0. Rooting seedlings and conditions as follows: light intensity 1500-2000LUX, a temperature of 25 ℃, photoperiod 5L: 19D, the culture period 50d. Statistical 50d shoots induced situation. Statistical results are shown in Table 3.

[0075] Table 3 seedlings and rooting medium screening

[0076]

[0077] NOTE: "-" indicates no callus; "+" indicates callus 0.1cm or less; "++" indicates callus 0.1cm above.

[0078] From the results in Table 3, when the seedling and rooting medium was modified MS medium...

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Abstract

The invention provides a culture medium combination for tissue culture and rapid propagation of Lilium medogense, a tissue culture and rapid propagation method and application, and belongs to the technical field of plant tissue rapid propagation. By optimizing the components of the culture medium, an induction culture medium and a differential culture medium are combined into one, a proliferation culture medium and a subculture medium are combined into one, and a strong seedling culture medium and a rooting culture medium are combined into one, so that the tissue culture steps of the Lilium medogenseare simplified, the rapid propagation of the Lilium medogense is realized, the problems of sporadic field distribution, rare population quantity and endangered extermination of the Lilium medogense are solved, a foundation is laid for comprehensive protection, development and continuous utilization of Lilium medogenseand preservation of germplasm resources, and the research and development blank of Lilium medogense in biotechnology is filled up.

Description

Technical field [0001] The present invention belongs to the field of plant tissue propagation techniques, particularly directed to medium composition for Motuo Lily Tissue Culture, Tissue Culture Methods and Applications. Background technique [0002] Medog lily (Lilium medogense) was published in 1985 by Mr. Liang Songjun new species of lily, Mr. Chen Weilie type specimen is a specimen collected from 1980 Medog County, Tibet. Currently Medog Lily has been in a situation on the verge of extinction, is a typical very small populations of wild plants, the expansion of the breeding and protection of great significance. There is no method for Tissue Culture of Lily Medog, therefore, an urgent need for a method Medog Lily Tissue Culture for. Inventive content [0003] To solve the above problems, the present invention provides a medium composition for Motuo Lily Tissue Culture, Tissue Culture Methods and Applications. The present invention provides a medium composition for Tissue Cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/15A01G24/28A01G24/23
CPCA01H4/008A01G24/15A01G24/23A01G24/28A01H4/002
Inventor 罗桂芬孙卫邦申建勇
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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