Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rapid detection kit for viruses of prawn leukoderma syndrome and application of rapid detection kit

A technology for detecting kits and syndrome viruses, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of high cost, time-consuming, complicated operation, etc., and achieve high sensitivity, simple and convenient operation, and specificity. strong effect

Inactive Publication Date: 2015-11-11
ZHEJIANG MARINE DEV RES INST
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a rapid detection kit for shrimp white spot syndrome virus in order to solve the defects of time-consuming, high cost and complicated operation of existing methods for detecting white spot syndrome virus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of application of the rapid detection kit of prawn white spot syndrome virus, comprises the following steps:

[0036] a) Sample preparation: take 10 mg of prawn tissue, put it in a centrifuge tube, add 100 μL of DNA extraction solution A, mash it for 1 min, cook at 98 ° C for 8 min; then cool to room temperature, centrifuge at 10,000 r / min for 3 min, and take the supernatant Place in another centrifuge tube, add 45 μL of DNA extraction solution B, mix well, and obtain the template DNA to be tested;

[0037] b) Nucleic acid amplification: according to the number of samples to be tested, set the number of LAMP reaction tubes required to be n, where n is the number of samples + one tube of positive control DNA + one tube of negative control DNA, and add 8 μL of LAMP pre-reaction to each tube solution, 1 μL of Bst polymerase and 12 μL of nuclease-free sterilized water, mix well, centrifuge at 2000r / min for 10s, then add 25μL of LAMP reaction stabilization solution, m...

Embodiment 2

[0046] A kind of application of the rapid detection kit of prawn white spot syndrome virus, comprises the following steps:

[0047] a) Sample preparation: take 30 mg of prawn tissue, put it in a centrifuge tube, add 110 μL of DNA extraction solution A, mash it for 2 minutes, cook at 99°C for 10 minutes; then cool to room temperature, centrifuge at 11000r / min for 4 minutes, and take the supernatant Place in another centrifuge tube, add 50 μL of DNA extraction solution B, mix well, and obtain the template DNA to be tested;

[0048] b) Nucleic acid amplification: according to the number of samples to be detected, set the number of LAMP reaction tubes required to be n, where n is the number of samples + one tube of positive control DNA + one tube of negative control DNA, and add 9 μL of LAMP pre-reaction to each tube solution, 2μL of Bst polymerase and 14μL of nuclease-free sterilized water, mix well, centrifuge at 2000r / min for 13s, then add 28μL of LAMP reaction stabilization so...

Embodiment 3

[0057] A kind of application of the rapid detection kit of prawn white spot syndrome virus, comprises the following steps:

[0058] a) Sample preparation: Take 50 mg of prawn tissue, put it in a centrifuge tube, add 120 μL of DNA extraction solution A, mash it for 2 minutes, cook at 100 ° C for 12 minutes; then cool to room temperature, centrifuge at 12000 r / min for 4 minutes, and take the supernatant Place in another centrifuge tube, add 55 μL of DNA extraction solution B, mix well, and obtain the template DNA to be tested;

[0059] b) Nucleic acid amplification: according to the number of samples to be detected, set the number of LAMP reaction tubes required to be n, where n is the number of samples + one tube of positive control DNA + one tube of negative control DNA, and add 9 μL of LAMP pre-reaction to each tube solution, 2 μL of Bst polymerase and 15 μL of nuclease-free sterilized water, mix well, centrifuge at 2000r / min for 15s, then add 30μL of LAMP reaction stabilizat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rapid detection kit for viruses of prawn leukoderma syndrome and an application of the rapid detection kit. The rapid detection kit comprises the following components: (a) DNA extracting solution A and DNA extracting solution B; (b) LAMP pre-reaction solution; (c) Bst polymerase; (d) LAMP reaction stabilizing solution; (e) developing liquid; (f) positive control DNA; and (g) negative control DNA. The rapid detection kit is strong in specificity and easy and convenient to operate, has higher sensitivity than a PCR method, and does not need an expensive PCR instrument, so that the cost is reduced, and the rapid detection kit is particularly suitable for a primary-level inspection and quarantine institution. A detection result of the detection method is simple and intuitive, and can be directly observed by naked eyes.

Description

technical field [0001] The invention relates to marine biological pathogen detection technology, in particular to a rapid detection kit for shrimp white spot syndrome virus and its application. Background technique [0002] Shrimp white spot syndrome virus is a large baculovirus that can widely infect seedlings, adults and broodstock of decapod crustaceans such as shrimp and crab. A large number of deaths have caused huge economic losses to the aquaculture industry. Therefore, a new technology is developed to detect WSSV (WSSV, WhiteSpot SyndromeVirus) quickly, accurately and sensitively, to strengthen the quarantine of seedlings and adult shrimp and the detection of aquaculture water environment, to prevent virus infection, cut off virus transmission, and ensure the health and sustainability of my country's shrimp farming industry development is important. [0003] In the past, there were mainly three methods for detecting prawn white spot syndrome virus: 1. Electron micro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/70C12Q1/6844C12Q2531/119C12Q2563/107
Inventor 杨会成李瑞雪周宇芳廖妙飞相兴伟
Owner ZHEJIANG MARINE DEV RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com