Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Surface plasma resonance method for detecting peanut allergen

A surface plasmon and peanut allergen technology, applied in the field of immunoassay, can solve the problems of time-consuming, labor-intensive, low-dose allergen detection without cost, low sensitivity, etc., and achieve the effect of convenient detection effect.

Inactive Publication Date: 2015-11-11
SHENZHEN UNIV
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a surface plasmon resonance method for detecting peanut allergens, which aims to solve the time-consuming and labor-intensive problems of the existing detection methods, low sensitivity and low dose of allergens. Undetectable or costly problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] The present invention provides a surface plasmon resonance method for detecting peanut allergens. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0034] The invention provides a surface plasmon resonance method for detecting peanut allergens, which comprises the steps of:

[0035] A, preparing peanut Arah1 protein;

[0036] B. Using peanut Arah1 protein as antigen, immunize animals to prepare anti-peanut Arah1 polyclonal antibody;

[0037] C. Using peanut Arah1 protein as an antigen, immunize animals, obtain hybridoma cells, and prepare anti-peanut Arah1 monoclonal antibodies through the hybridoma cells;

[0038] D. Immobilize the anti-peanut Arah1 monoclonal antibody on the detection chip by coup...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a surface plasma resonance method for detecting a peanut allergen. The method specifically comprises the steps: respectively preparing an anti-peanut Ara h1 monoclonal antibody and an anti-peanut Ara h1 polyclonal antibody, fixing the anti-peanut Ara h1 monoclonal antibody on a detection chip by means of a coupling method, facilitating peanut Ara h1 proteins of different concentrations to flow by the detection chip, adding the anti-peanut Ara h1 polyclonal antibody which is used for magnifying a signal, so that a purpose of rapidly and efficiently detecting a main allergen protein of the peanut can be achieved. The method has characteristics of high sensitivity, high accuracy, rapidness in detection and the like and can become an important technical means for detecting the peanut allergen.

Description

technical field [0001] The invention relates to the technical field of immune analysis, in particular to a surface plasmon resonance method for detecting peanut allergens. Background technique [0002] Peanuts and their products are one of the eight categories of foods identified by FAO / WHO as causing human food allergies. In the European and American food labeling laws, peanuts are also stipulated as food allergen ingredients that must be labeled. Arah1 protein is the most important allergen in peanut. [0003] The detection method of peanut allergen is mainly aimed at the specific allergen protein or the DNA fragment encoding the allergen in peanut as the detection target. Therefore, detection methods are mainly divided into two categories: methods based on detection of genes and methods based on detection of proteins. Gene-based methods rely on polymerase chain reaction (PCR) to amplify specific DNA fragments, such methods include qualitative PCR and real-time fluoresc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 吴序栎刘志刚何伟逸李瑶
Owner SHENZHEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com