Preparing method for high-concentration NAA gel

Abstract

The invention discloses a preparing method for high-concentration NAA gel, and belongs to the technical field of plant biology. The preparing method includes the steps that NAA mother liquid with the concentration of 1 mg / mL is prepared; the mother liquid is diluted into high-concentration NAA working liquid with the concentration of 500 mg / L; the working liquid is prepared into high-concentration NAA gel confining liquid containing sodium carboxymethylcellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8%.

Description

technical field [0001] The invention discloses a preparation method of high-concentration NAA gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as NAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propagation or ro...

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients

However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.