Ex-vivo expansion culture medium of umbilical cord blood hematopoietic stem cells and application thereof

A technology for hematopoietic stem cells and in vitro expansion, applied in the field of in vitro expansion medium for umbilical cord blood hematopoietic stem cells, can solve the problems of low implantability, hematopoietic reconstruction ability, and low cell proliferation rate, and achieve implantability and hematopoietic system Improvement of the reconstruction ability, high proliferation rate, and the effect of improving the proliferation ability

Active Publication Date: 2015-12-02
广东美赛尔细胞生物科技有限公司
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the defects of low cell proliferation rate in the in vitro expansion medium of umbilical cord blood hematopoietic stem cells in the prior art, and low engraftment ability and hematopoietic reconstruction ability of the umbilical cord blood hematopoietic stem cells cultured in vitro, this The object of the invention is to provide a umbilical cord blood hematopoietic system with a high proliferation rate of hematopoietic stem cells, which can significantly improve the implantation ability of the hematopoietic stem cells transplanted into the recipient and the reconstruction ability of the hematopoietic system, and can better maintain the stemness of the stem hematopoietic cells. Stem cell in vitro expansion medium to solve the above problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ex-vivo expansion culture medium of umbilical cord blood hematopoietic stem cells and application thereof
  • Ex-vivo expansion culture medium of umbilical cord blood hematopoietic stem cells and application thereof
  • Ex-vivo expansion culture medium of umbilical cord blood hematopoietic stem cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Effects of Medium Containing Different Concentrations of Berberine on the Proliferation of CD34+CD38-Lin- Cells in Vitro

[0027] 1. Test materials: Umbilical cord blood was collected from the Department of Obstetrics and Gynecology of Huaqiao Hospital, and the mononuclear cell isolation kit was purchased from Beijing Suolaibao Technology Co., Ltd. (article number: P9260).

[0028] 2. Test method: the collected umbilical cord blood was separated from mononuclear cells with a mononuclear cell isolation kit to obtain mononuclear cells from umbilical cord blood, and then CD34+CD38-Lin- cells were separated by immunomagnetic bead cell sorting, and used Cell purity was identified by flow cytometry.

[0029] Release berberine into 2, 5, 7, 10ug / ml drug solutions with IMEM basal medium, and the cells separated from P1 generation were treated with 10 4Cells / mL were inoculated in 96-well plates, and the in vitro expansion medium of umbilical cord blood hematopoieti...

Embodiment 2

[0031] Embodiment two, In vitro expansion medium of umbilical cord blood hematopoietic stem cells

[0032] The medium for in vitro expansion of umbilical cord blood hematopoietic stem cells, including IMEM basal medium, also includes the following components and their concentrations: fetal bovine serum 0.05mg / mL, thrombopoietin 15ng / mL, stem cell factor 100ng / mL, human FMS-like Tyrosine kinase 3 ligand 33ng / ml, interleukin-10.2ng / ml, interleukin-60.2ng / ml, stem cell growth factor 0.2ng / ml and berberine 2ug / ml.

[0033] Preparation method: Add fetal bovine serum, thrombopoietin, stem cell factor, human FMS-like tyrosine kinase 3 ligand, interleukin-1, interleukin-6, stem cell growth factor and small Berine, mixed evenly, passed through membrane to sterilize.

Embodiment 3

[0034] Embodiment three, In vitro expansion medium of umbilical cord blood hematopoietic stem cells

[0035] The medium for in vitro expansion of umbilical cord blood hematopoietic stem cells, including IMEM basal medium, also includes the following components and their concentrations: fetal bovine serum 0.1mg / mL, thrombopoietin 25ng / mL, stem cell factor 120ng / mL, human FMS-like Tyrosine kinase 3 ligand 45ng / ml, interleukin-10.3ng / ml, interleukin-60.6ng / ml, stem cell growth factor 0.5ng / ml and berberine 5ug / ml.

[0036] The preparation method is similar to Example 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of stem cells, and relates to an ex-vivo expansion culture medium of umbilical cord blood hematopoietic stem cells and an application thereof. The ex-vivo expansion culture medium comprises an IMEM basal culture medium, serum, thrombopoietin, stem cell factors, FMS-liketyrosinc kinase 3 ligand, interleukin-1, interleukin-6, stem cell growth factors and berberine. The ex-vivo expansion culture medium of the umbilical cord blood hematopoietic stem cells has the advantages of being high in hematopoietic stem cell proliferation rate, capable of significantly improving the implantation capacity of the hematopoietic stem cells transplanted into a receptor and the reconstruction capacity of a hematopoietic system and capable of well maintaining the properties of the hematopoietic stem cells and is an ideal ex-vivo expansion culture medium of the umbilical cord blood hematopoietic stem cells.

Description

Technical field [0001] The present invention is a stem cell technology field, involving an in vitro amplification culture base and its application that involves an umbilical cord blood stem cell. Background technique [0002] HematopoieticsCells (HSCS) is a adult stem cell in the blood system. It is a heterogeneous group. It has long -term self -renewal capabilities and the potential of various mature blood cells.Because hematopoietic stem cells have the potential to differentiate, transplant hematopoietic stem cells into the body is a effective way to treat malignant tumors such as leukemia, lymphoma, metabolic diseases, autoimmune diseases, and congenital immune defects.Reduce the pain of patients.However, due to the small number of hematopoietic stem cells in the body, the application of hematopoietic stem cells in clinical is greatly limited. [0003] Bone marrow, peripheral blood, and umbilical blood are important sources of hematopoietic stem cells. Umbilical blood between ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0789
Inventor 王一飞程江王巧利廖晓凤刘秋英
Owner 广东美赛尔细胞生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap