Method for breeding mulberry polyploids through chemical mutagenesis

A chemical mutagenesis and polyploidy technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problem of poor economic traits, few natural tetraploid mulberry resources, and can not meet the needs of artificial triploid new mulberry. Variety breeding parent requirements and other issues, to achieve the effect of improving economic efficiency and wide variation spectrum

Inactive Publication Date: 2015-12-09
SERICULTURAL RES INST ANHUI ACADEMY OF AGRI SCI
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Problems solved by technology

Mulberry diploid mulberry resources are abundant, and it is easy to select germplasm resources with good economic traits as breeding parents. However, natural tetraploid mulberry resources are very few, and they are wild types. Requirements for Parents of Tixin Mulberry Variety Breeding

Method used

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Embodiment Construction

[0011] A method for chemically induced polyploid breeding of mulberry trees, comprising the following:

[0012] 1. Material collection: Select mulberry diploid hybridized adult mulberry shoots that have been growing vigorously, full of growth points, and free from diseases and insect pests for more than 2 years as the material, and cut the material mulberry plants in spring. In the middle and late April, select the vigorous growth for preparation The processed mulberry buds are discarded;

[0013] 2. Chemical mutagen: The mutagen is a mixture of 0.4% colchicine (colchicine) and 3% dimethyl sulfoxide (DMSO); colchicine is the main mutagenic agent and dimethyl sulfoxide is the auxiliary agent. Increase the permeability of colchicine and improve the induction effect;

[0014] 3. Preliminary chromosome induction: use a syringe to inject about 0.1ml of chemical mutagen into the bud or growth point to induce polyploid chromosomes;

[0015] 4. Chromosomal induction again: Diligentl...

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Abstract

The invention discloses a method for breeding mulberry polyploids through chemical mutagenesis. A mixed solution of 0.4% of colchicine and 3% of DMSO is employed as a mutagenic agent, new shoots of mature mulberries more than two years old, in vigorous growth, with plump growing points and without insect diseases are subjected to chemical mutagenesis processing, and new mulberry strains with genetic stability and with excellent or special biological characters and economic characters are obtained through generation selection. Through the method, high-frequency polyploid mutant plants can be obtained, the variation spectrum is wide, excellent new mulberry strains with genetic stability can be obtained, which is helpful for improvement of mulberry species. In addition, orientation selection of parents can be carried out according to actual demands of silkworm production, furthermore excellent new species with excellent biological characters and economic characters are obtained, demands of diversification and all-year silkworm breeding production for multiple times to mulberry species are met, and the economic benefit of the silkworm industry is raised.

Description

technical field [0001] The invention belongs to the field of mulberry breeding, and in particular relates to a method for chemical mutagenesis and breeding of mulberry polyploids. Background technique [0002] Mulberry is a kind of important economic forest tree species, perennial woody plant, and mulberry leaf is the only feed that can make the normal metabolism of silkworm. Studies have shown that polyploid mulberry trees, especially triploid mulberry trees, have the characteristics of high leaf yield, high leaf quality, and strong stress resistance. Once the excellent polyploid mulberry is obtained, it can be propagated asexually by grafting, cuttings, etc. to maintain its excellent species. Therefore, mulberry polyploidy breeding has great significance and is easy to reproduce, and has been paid attention to by the majority of mulberry breeding scientific and technological workers. [0003] Most of the natural triploid mulberries are produced by natural hybridization, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
Inventor 李瑞雪王钰婷汪泰初王伟高新文胡钢
Owner SERICULTURAL RES INST ANHUI ACADEMY OF AGRI SCI
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