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Primer set, kit and detection method for multiplex PCR detection of Staphylococcus aureus and Salmonella in food

A technology for Staphylococcus and Salmonella, which is applied in the field of primer sets for multiplex PCR detection, can solve problems such as not fully meeting the national food safety standards, and achieve the effects of wide application market prospects, short detection time, and simple operation

Active Publication Date: 2019-08-02
COFCO NUTRITION & HEALTH RES INST +1
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

It can be seen that the existing technology cannot fully meet the needs of national food safety standards

Method used

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  • Primer set, kit and detection method for multiplex PCR detection of Staphylococcus aureus and Salmonella in food
  • Primer set, kit and detection method for multiplex PCR detection of Staphylococcus aureus and Salmonella in food
  • Primer set, kit and detection method for multiplex PCR detection of Staphylococcus aureus and Salmonella in food

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Embodiment Construction

[0052] According to some embodiments of the present invention, in addition to the primer set for multiplex PCR detection of the present invention, the kit for multiplex PCR detection of the present invention may further include components such as reaction buffer, 4 kinds of deoxynucleoside triphosphates, DNA polymerase, etc. .

[0053] According to some preferred embodiments of the present invention, in addition to the primer set for multiplex PCR detection of the present invention, the kit for multiplex PCR detection of the present invention may further include a PCR reaction base solution with the following components: Tris-HCl; KCl; MgCl 2 ; dATP, dTTP, dGTP, and dCTP; and Taq DNA polymerase.

[0054] According to some more preferred embodiments of the present invention, in the multiplex PCR detection kit of the present invention, each component is contained in the final PCR reaction solution of 25 μL at the following concentration:

[0055]

[0056] Wherein, the femA g...

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Abstract

The invention belongs to the field of food microbe safety detection, and provides a primer group for multi-PCR detection of Staphylococcus aureus and salmonellae in a food, a kit and a detection method. The multi-PCR detection primer group, the kit and the detection method can realize the technical effects of wide detection coverage range, strong specificity and high sensitivity through combined utilization of species specificity discriminating genes and drug resistance related genes of Staphylococcus aureus and salmonellae, and have the advantages of short detection time, simple operation, low device requirements, high flux, saving of a large amount of manpower, material resources and financial resources, suitableness for rapid detection requirements, and easy popularization and application in practical production.

Description

technical field [0001] The invention belongs to the field of food microbe safety detection, and relates to a primer set, a kit and a method for rapidly detecting and identifying pathogenic bacteria in food by using multiple PCR technology. Specifically, the present invention relates to a multiplex PCR detection primer set, kit and detection method for Staphylococcus aureus and Salmonella sp. in foods. Background technique [0002] In recent years, food safety incidents have occurred frequently, and food safety issues have become more and more concerned and valued by the public. Many food-borne pathogens are transmitted through water and food and cause food-borne diseases, and their morbidity and mortality are gradually increasing. Worldwide, more than 30% of the population is infected with foodborne illness each year, costing billions of dollars. Preventing and controlling the occurrence and spread of foodborne diseases has become one of the important measures to solve soc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/686C12Q1/14C12Q1/04C12N15/11C12R1/445C12R1/42
Inventor 蔡军李慧刘洋欧竞堃石嵩杨凯
Owner COFCO NUTRITION & HEALTH RES INST
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