Section-position using method adopting high-concentration 2,4-D and glycerinum gel

Abstract

The invention discloses a section-position using method adopting high-concentration 2,4-D and glycerinum gel and belongs to the technical field of plant biology. At first, 2,4-D mother liquor of 1 mg / mL is prepared; the mother liquor is diluted into a high-concentration 2,4-D working solution of 500 mg / L; the working solution is prepared into high-concentration 2,4-D and glycerinum gel confining liquid containing sodium carboxymethyl cellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8%; a branch is inserted into a culture medium; the gel confining liquid is dripped into an axillary bud of the healthy and strong branch.

Description

technical field [0001] The invention discloses a method for using high-concentration 2,4-D and glycerol gel at nodes, belonging to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as 2,4-D, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro pr...

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients

However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.