A kind of Ganoderma lucidum triterpene acid active part and its preparation method and application

A technology of ganoderma triterpene acids and active parts, which is applied in the field of ganoderma extracts, can solve the problems that the active functional components are not very clear, there is no refining of ganoderma extracts, etc., and achieve obvious sedative and tranquilizing effects, which is conducive to market promotion and utilization. The effect of broad application prospects

Active Publication Date: 2018-07-31
杭州医学院资产经营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, although Ganoderma lucidum is a precious fungus and algae medicinal material in my country, and its "replenishing qi and tranquilizing" effect has been used medicinally for thousands of years, modern pharmacology has not yet clarified the corresponding active and functional components, and most pharmacological experiments only stay on the original medicinal material. On the basis of Ganoderma lucidum extract and crude extract, there is no report that Ganoderma lucidum extract is further refined to the active part and used to prepare sedative and tranquilizing health care products and medicines

Method used

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  • A kind of Ganoderma lucidum triterpene acid active part and its preparation method and application
  • A kind of Ganoderma lucidum triterpene acid active part and its preparation method and application
  • A kind of Ganoderma lucidum triterpene acid active part and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1 (preparation of ganoderma triterpene acid active site)

[0044] (1) Take Ganoderma lucidum fruiting body medicinal material (Zhejiang Wuyangtang Pharmaceutical Co., Ltd.), dry at 65°C, pulverize, and pass through a 100-mesh sieve to obtain dry powder; add distilled water to the dry powder at a material-to-liquid ratio of 1g: 20mL, and heat at a temperature of 50°C and pH5. 5, add 3.0% of the compound enzyme relative to the mass of the dry powder, the compound enzyme is composed of 50% neutral protease, 25% cellulase and 25% pectinase in percentage by weight, and the enzymolysis time is 6h. Enzyme, cooling, suction filtration, obtain enzymatic hydrolysis solution, set aside;

[0045] (2) Take the enzymolysis solution prepared in the above step (1), add petroleum ether to extract and remove fat, then continue to add ethyl acetate to the enzymolysis solution after degreasing to extract, to obtain the extraction part of ethyl acetate, and evaporate the ethyl ac...

Embodiment 2

[0049] Embodiment 2 (preparation of ganoderma triterpene acid active site)

[0050] (1) Take Ganoderma lucidum fruiting body medicinal material (Zhejiang Wuyangtang Pharmaceutical Co., Ltd.), dry at 65°C, pulverize, and sieve through a 100-mesh sieve to obtain dry powder; add distilled water to the dry powder at a material-to-liquid ratio of 1g︰15mL, and heat at a temperature of 40°C and pH4. Under the condition of 0, add 2.0% compound enzyme relative to the mass of the dry powder, the compound enzyme is composed of 30% neutral protease, 35% cellulase and 35% pectinase in weight percentage, the enzymolysis time is 5h, and the Enzyme, cooling, suction filtration, obtain enzymatic hydrolysis solution, set aside;

[0051] (2) Take the enzymolysis solution prepared in the above step (1), add petroleum ether to extract and remove fat, then continue to add ethyl acetate to the enzymolysis solution after degreasing to extract, to obtain the extraction part of ethyl acetate, and evapo...

Embodiment 3

[0055] Embodiment 3 (preparation of ganoderma triterpene acid active site)

[0056] (1) Take Ganoderma lucidum fruiting body medicinal material (Zhejiang Wuyangtang Pharmaceutical Co., Ltd.), dry at 65°C, pulverize, and pass through a 100-mesh sieve to obtain dry powder; add distilled water to the dry powder at a material-to-liquid ratio of 1g: 25mL, and heat at a temperature of 60°C and pH6. Under the condition of 0, add 4.0% compound enzyme relative to the mass of the dry powder, the compound enzyme is composed of 80% neutral protease, 10% cellulase and 10% pectinase in weight percentage, the enzymolysis time is 7h, and the Enzyme, cooling, suction filtration, obtain enzymatic hydrolysis solution, set aside;

[0057] (2) Take the enzymolysis solution prepared in the above step (1), add petroleum ether to extract and remove fat, then continue to add ethyl acetate to the enzymolysis solution after degreasing to extract, to obtain the extraction part of ethyl acetate, and evapo...

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Abstract

The invention discloses a ganoderma lucidum triterpene acid active part and its preparation method and its application in the preparation of sleep-improving health care products and sedative and tranquilizing medicines. The method comprises: crushing the ganoderma lucidum fruiting body medicinal material to obtain dry powder, and adding compound enzymes to the dry powder The enzymolysis liquid was obtained by inactivating the enzyme, cooling, and suction filtration; the enzymolysis liquid was added to petroleum ether for extraction to remove fat, and then ethyl acetate was added for extraction to obtain the ethyl acetate extract of Ganoderma lucidum; the ethyl acetate extract of Ganoderma lucidum was first Alkaline, then wash with water until neutral to obtain an alkalized water layer, then acidify the alkalized water layer, and then extract crude ganoderma acid through ethyl acetate; separate the crude ganoderma acid through a silica gel column, and use chloroform and methanol system gradient Eluted, identified by thin-layer chromatography, filtered, and evaporated to dryness, the active part of triterpene acid of Ganoderma lucidum was obtained. The active part of the ganoderma triterpene acid of the present invention can be obtained by enzymatic hydrolysis with complex enzymes, and then through extraction, separation and other steps, and can be used to prepare sleep-improving health care products and sedative and tranquilizing drugs.

Description

technical field [0001] The invention relates to the technical field of Ganoderma lucidum extract, in particular to an active part of Ganoderma lucidum triterpene acid and its preparation method and its application in the preparation of sleep-improving health care products and tranquilizing drugs. Background technique [0002] Sleep is an essential physiological process for humans and higher animals, and is the basis for maintaining physical strength and health. Insomnia is a common sleep disorder that refers to the inability to fall asleep or stay asleep. Chronic insomnia probably affects about 15% of the population, and transient insomnia affects about 80% of the population each year. At present, the treatment of insomnia is mainly to take artificial synthetic drugs, but synthetic drugs have serious drug dependence, cognitive and motor impairment, drug withdrawal rebound and other adverse reactions. Therefore, there is an urgent need to find and develop safe and effective...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/575A61K36/074A61P25/20A23L33/10
Inventor 么春艳吴学谦俞盈许海顺徐娟熊科辉肖丛梅
Owner 杭州医学院资产经营有限公司
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