A kind of Ganoderma lucidum triterpene acid active part and its preparation method and application
A technology of ganoderma triterpene acids and active parts, which is applied in the field of ganoderma extracts, can solve the problems that the active functional components are not very clear, there is no refining of ganoderma extracts, etc., and achieve obvious sedative and tranquilizing effects, which is conducive to market promotion and utilization. The effect of broad application prospects
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Embodiment 1
[0043] Embodiment 1 (preparation of ganoderma triterpene acid active site)
[0044] (1) Take Ganoderma lucidum fruiting body medicinal material (Zhejiang Wuyangtang Pharmaceutical Co., Ltd.), dry at 65°C, pulverize, and pass through a 100-mesh sieve to obtain dry powder; add distilled water to the dry powder at a material-to-liquid ratio of 1g: 20mL, and heat at a temperature of 50°C and pH5. 5, add 3.0% of the compound enzyme relative to the mass of the dry powder, the compound enzyme is composed of 50% neutral protease, 25% cellulase and 25% pectinase in percentage by weight, and the enzymolysis time is 6h. Enzyme, cooling, suction filtration, obtain enzymatic hydrolysis solution, set aside;
[0045] (2) Take the enzymolysis solution prepared in the above step (1), add petroleum ether to extract and remove fat, then continue to add ethyl acetate to the enzymolysis solution after degreasing to extract, to obtain the extraction part of ethyl acetate, and evaporate the ethyl ac...
Embodiment 2
[0049] Embodiment 2 (preparation of ganoderma triterpene acid active site)
[0050] (1) Take Ganoderma lucidum fruiting body medicinal material (Zhejiang Wuyangtang Pharmaceutical Co., Ltd.), dry at 65°C, pulverize, and sieve through a 100-mesh sieve to obtain dry powder; add distilled water to the dry powder at a material-to-liquid ratio of 1g︰15mL, and heat at a temperature of 40°C and pH4. Under the condition of 0, add 2.0% compound enzyme relative to the mass of the dry powder, the compound enzyme is composed of 30% neutral protease, 35% cellulase and 35% pectinase in weight percentage, the enzymolysis time is 5h, and the Enzyme, cooling, suction filtration, obtain enzymatic hydrolysis solution, set aside;
[0051] (2) Take the enzymolysis solution prepared in the above step (1), add petroleum ether to extract and remove fat, then continue to add ethyl acetate to the enzymolysis solution after degreasing to extract, to obtain the extraction part of ethyl acetate, and evapo...
Embodiment 3
[0055] Embodiment 3 (preparation of ganoderma triterpene acid active site)
[0056] (1) Take Ganoderma lucidum fruiting body medicinal material (Zhejiang Wuyangtang Pharmaceutical Co., Ltd.), dry at 65°C, pulverize, and pass through a 100-mesh sieve to obtain dry powder; add distilled water to the dry powder at a material-to-liquid ratio of 1g: 25mL, and heat at a temperature of 60°C and pH6. Under the condition of 0, add 4.0% compound enzyme relative to the mass of the dry powder, the compound enzyme is composed of 80% neutral protease, 10% cellulase and 10% pectinase in weight percentage, the enzymolysis time is 7h, and the Enzyme, cooling, suction filtration, obtain enzymatic hydrolysis solution, set aside;
[0057] (2) Take the enzymolysis solution prepared in the above step (1), add petroleum ether to extract and remove fat, then continue to add ethyl acetate to the enzymolysis solution after degreasing to extract, to obtain the extraction part of ethyl acetate, and evapo...
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